LOCUS       sequence-174443-       13172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR390 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM390
FEATURES             Location/Qualifiers
     source          1..13172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2387..2433
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(2452..2466,2657..4474)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          2467..2656
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4481..4498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4533..4785
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4807..4831
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6132..6761
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7190..8263
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8329..8523
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8759..8781)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8867..9007
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8922..8941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9022..9039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9193..9781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9868..10662)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11087..11111
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11189..11363
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11404..12429)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12496..13172)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttggcg
      361 ctatccccta tcctgagctt gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgg cgctatcccc tatcctgagc ttgaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcgccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct ccagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gcaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaac tgcaggcatg caagcttcct tttgtctccc ttttgtctcc cttttgtctc
     2341 ccttttgtct cccttttgtc tcccttttgt ctcccttttg tctctcgcaa gacccttcct
     2401 ctatataagg aagttcattt catttggaga gaacacgggg gactcttgac catggtagat
     2461 ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct tttctctttt
     2521 tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga ttggttatgg
     2581 tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt gtctatgatg
     2641 atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa cccgtgaaat
     2701 caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
     2761 gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
     2821 cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga
     2881 agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac
     2941 tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
     3001 gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
     3061 ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
     3121 aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg
     3181 cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
     3241 tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt
     3301 cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg
     3361 gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact
     3421 gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg
     3481 gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct actttactgg
     3541 ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg tgctgatggt
     3601 gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc
     3661 ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac
     3721 tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa
     3781 agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat
     3841 taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa
     3901 cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac
     3961 gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca
     4021 caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta
     4081 tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg
     4141 gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg
     4201 gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat
     4261 gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc
     4321 cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac
     4381 tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa
     4441 cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc accaccacgt
     4501 gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     4561 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     4621 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     4681 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     4741 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
     4801 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     4861 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     4921 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     4981 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     5041 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     5101 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     5161 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     5221 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     5281 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     5341 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     5401 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     5461 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     5521 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     5581 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     5641 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     5701 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     5761 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     5821 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     5881 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     5941 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     6001 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     6061 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     6121 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     6181 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     6241 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     6301 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     6361 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     6421 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     6481 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     6541 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     6601 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     6661 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     6721 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     6781 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     6841 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6901 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6961 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     7021 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     7081 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     7141 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     7201 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     7261 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     7321 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     7381 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     7441 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     7501 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     7561 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     7621 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     7681 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     7741 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     7801 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     7861 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     7921 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     7981 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     8041 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     8101 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     8161 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     8221 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     8281 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     8341 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     8401 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     8461 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     8521 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     8581 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     8641 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     8701 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8761 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8821 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     8881 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     8941 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9001 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9061 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9601 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9841 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     9901 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     9961 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    10021 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10081 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    10141 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    10201 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    10261 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    10321 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    10381 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    10441 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    10501 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    10561 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    10621 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    10681 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    10741 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    10801 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    10861 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    10921 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    10981 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11041 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11101 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11161 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    11221 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    11281 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    11341 actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg gtcggcatct
    11401 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
    11461 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
    11521 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
    11581 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
    11641 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
    11701 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
    11761 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
    11821 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
    11881 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
    11941 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
    12001 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
    12061 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
    12121 caggtcttgc aacgtgacac cctgtgaacg gcgggagatg caataggtca ggctctcgct
    12181 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
    12241 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
    12301 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
    12361 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
    12421 ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata gatttgtaga
    12481 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    12541 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    12601 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    12661 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    12721 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    12781 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    12841 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    12901 agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    12961 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    13021 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    13081 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    13141 ttcccgatat taccctttgt tgaaaagtct ca
//
