LOCUS       sequence-174445-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP7 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP7.v1 puro
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     502..521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     755..772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    889..910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(925..930,931..948,949..955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    963..979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     968..990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     987..1003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     987..1003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1022..1042
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1024..1042
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1070..1296
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1297..1477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1524..1649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2142..2375
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2560..2604
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2893..2987
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2904..2923
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2941..2959
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2969..2987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3088..3205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3254..3764
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3683..3701
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3773..4345
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4353..4372
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4400..4418
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4429..5049
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4502..4521)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5084..5658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5252..5269)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5479..5498
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5678..6277
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5678..5697)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6174..6194
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6405..6638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6710..6831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6747..6766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6801..6820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6871..7006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7027..7045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7055..7072)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7055..7071)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7086)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7213..7668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7300..7319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7510..7531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7694..7798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7799..7867,7868..8659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8017..8036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
       61 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      781 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      841 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      901 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      961 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     1021 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     1081 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     1141 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     1201 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     1261 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     1321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1441 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1501 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1561 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1621 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1681 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1741 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1801 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1861 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1921 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1981 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     2041 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     2101 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     2161 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     2221 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     2281 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     2341 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     2401 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     2461 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     2521 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     2581 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2641 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2701 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2761 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2821 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct
     2881 cgagcggccg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
     2941 tccctatcag tgatagagac ttataagttc cctatcagtg atagagacac cggccggcca
     3001 tttgtggaat acaactgcag ttgtattcca caaatggccg gtttttgaat tctcgacctc
     3061 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     3121 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     3181 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     3241 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     3301 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     3361 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     3421 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     3481 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     3541 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     3601 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     3661 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     3721 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ctgtgagttt
     3781 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     3841 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     3901 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     3961 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     4021 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     4081 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     4141 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     4201 tagaaacaac tacatcctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     4261 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     4321 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     4381 tcattttggc aaagaattgt aatacgactc actatagggc gaattgatat gtctagatta
     4441 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     4501 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     4561 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     4621 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     4681 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     4741 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     4801 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     4861 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     4921 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     4981 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     5041 agtgggtccg cgtacagcgg ctcccgggag ttctagggat ctgcccctct ccctcccccc
     5101 ccccntaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5161 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5221 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5281 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5341 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5401 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     5461 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     5521 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     5581 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     5641 tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca agcccacggt
     5701 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5761 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5821 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5881 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5941 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6001 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6061 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6121 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6181 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6241 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     6301 cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa
     6361 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6421 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6661 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6721 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6781 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6841 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     7021 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7081 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7141 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7201 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7261 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7501 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7561 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7621 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7681 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7741 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7801 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7861 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7921 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7981 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8041 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8101 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8161 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8221 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8281 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8341 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8401 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8461 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8521 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8581 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8641 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagat
//
