LOCUS       sequence-174446-       14970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR159 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14970)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM159
FEATURES             Location/Qualifiers
     source          1..14970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     409..428
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        409..427
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        835..1180
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1341..1517
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2079..2097)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2080..2097)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(2858..2863,2864..2880,2881..2886))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3169..3185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3193..3209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3217..3223,3224..3241,3242..3247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3262..3283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3474..4150
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4217..5242
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5283..5457
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5535..5559
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5984..6778
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6865..7453
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7607..7624
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7639..7779
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7705..7724)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7865..7887
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8123..8317
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8383..9456)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9885..10514)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11815..11839
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11861..12113)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12148..12165)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12172..13989,14180..14194))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          13990..14179
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(14211..14556)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14918..14940
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14932..14949
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14933..14949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgaca tgattacgaa ttcgagctcg gtacccgggg
      241 atcctctagc tagattaatt aagtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      301 tcaaggagat cctttggatc acaaaaaaaa agcccgctca ttaggcgggt taaattcccg
      361 ggtactgcag ccgattcatt aatgcaggtt aacctggctt atcgaaatta atacgactca
      421 ctatagggag accggcagat ctgatcccta ctccaaaaat gtcaaagata cagtctcaga
      481 agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt
      541 ccattgccca gctatctgtc acttcatcga aaggacagtg gaaaaggaag gtggctccta
      601 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg
      661 tcccaaagat ggacccccac ccacgaggag catcgtggga aaagaagacg ttccaaccac
      721 gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
      781 ccacccctac tccaaaaatg tcaaagatac tgtctcagaa gaccaaaggg ctattgagac
      841 ttttcaacaa agggtaattt cgggaaacct cctcggattc cattgcccag ctatctgtca
      901 cctcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa
      961 aggaaaggct atcattcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     1021 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     1081 atgtgacatc tccactgaca taagggatga cgcacaaccc cactatcctt cgcaagaccc
     1141 ttcctctata taaggaagtt catttcattt ggagaggaca gcccaagctt cagagctccc
     1201 tctatcaatc caaagttgtt gttgttatgg tctaatttaa atatggtcta aagaagaaga
     1261 attagagctc cctctatcaa tccaaggaat tcggtacgct gaaatcacca gtctctctct
     1321 acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc ccgataaggg
     1381 aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     1441 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     1501 gtactaaaat ccagatctcc taaagtccct atagatcttt gtcgtgaata taaaccagac
     1561 acgagacgac tagacctgga gcccagacgc cgttcgaagc tagaagtacc gcttaggcag
     1621 gaggccgtta gggaaaagat gctaaggcag ggttggttac gttgactccc ccgtaggttt
     1681 ggtttaaata tgatgaagtg gacggaagga aggaggaaga caaggaagga taaggttgca
     1741 ggccctgtgc aaggtaagaa gatggaaatt tgatagaggt acgctactat acttatacta
     1801 tacgctaagg gaatgattgt atttataccc tataccccct aataacccct tatcaattta
     1861 agaaataatc cgcataagcc cccgcttaaa aattggtatc agagccatga ataggtctat
     1921 gaccaaaact caagaggata aaacctcacc aaaatacgaa agagtcctta actctaaaga
     1981 taaaagatct ttcaagatca aaactagttc cctcacaccg gtgacgggga tcgcatgcga
     2041 cctctagacc atcacgttgg agtttcagct gctcgagttc tatagtgtca cctaaatcgt
     2101 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttcttacgt cgacgtacca
     2161 attacgcccc gccctgtcac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2221 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2281 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2341 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2401 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2461 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2521 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat attaccagct
     2581 caccgtcttt cattgccata cggaactccg gatgagcatt catcaggcgg gcaagaatgt
     2641 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2701 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gccccaaaat
     2761 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tctttctcca
     2821 ttttagcttc cttagctcct gaaaatctcg ataactcatt aaagcttatc gatgataagc
     2881 tgtcaaacat gagaatttag cccgcctaat gagcgggctt tttgttgtga tccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttcccccc tggaagctcc
     3061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttcttaat
     3121 taatctagct agaggatccc cgggtaccga gctcgaattc gtaatcatgt catagctgtt
     3181 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3241 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3301 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3361 ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact
     3421 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
     3481 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     3541 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     3601 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     3661 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     3721 tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
     3781 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     3841 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
     3901 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
     3961 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     4021 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
     4081 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     4141 gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc
     4201 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     4261 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     4321 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     4381 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     4441 ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct cccgccgttc
     4501 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tacaaccggt
     4561 cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     4621 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     4681 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     4741 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     4801 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     4861 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     4921 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     4981 gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg accaactcta
     5041 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     5101 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     5161 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     5221 tcgtccgagg gcaaagaaat agagtagatg ccgaccggga tctgtcgatc gacaagctcg
     5281 agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg
     5341 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     5401 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc
     5461 cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5521 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5581 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5641 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5701 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5761 gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg
     5821 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     5881 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     5941 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     6001 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     6061 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     6121 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     6181 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     6241 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     6301 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     6361 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     6421 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     6481 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     6541 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     6601 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     6661 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     6721 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     6781 cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6841 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6901 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6961 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7021 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7081 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7141 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7201 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7261 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7321 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7381 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7441 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7501 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7561 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7621 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7681 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7741 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     7801 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7921 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     7981 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     8041 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     8101 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     8161 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     8221 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     8281 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     8341 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     8401 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     8461 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     8521 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     8581 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     8641 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     8701 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     8761 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     8821 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     8881 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     8941 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     9001 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     9061 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     9121 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     9181 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     9241 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     9301 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     9361 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     9421 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     9481 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
     9541 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     9601 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     9661 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     9721 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     9781 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     9841 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     9901 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     9961 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    10021 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    10081 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    10141 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    10201 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    10261 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    10321 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    10381 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    10441 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    10501 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    10561 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    10621 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    10681 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    10741 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    10801 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    10861 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    10921 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    10981 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    11041 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    11101 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    11161 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    11221 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    11281 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    11341 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    11401 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    11461 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    11521 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    11581 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    11641 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    11701 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    11761 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
    11821 ccgccaatat atcctgtcaa acactgatag tttaattccc gatctagtaa catagatgac
    11881 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
    11941 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
    12001 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
    12061 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa
    12121 ttcgagctgg tcacctgtaa ttcacacgtg gtggtggtgg tggtggctag cttgtttgcc
    12181 tccctgctgc ggtttttcac cgaagttcat gccagtccag cgtttttgca gcagaaaagc
    12241 cgccgacttc ggtttgcggt cgcgagtgaa gatccctttc ttgttaccgc caacgcgcaa
    12301 tatgccttgc gaggtcgcaa aatcggcgaa attccatacc tgttcaccga cgacggcgct
    12361 gacgcgatca aagacgcggt gatacatatc cagccatgca cactgatact cttcactcca
    12421 catgtcggtg tacattgagt gcagcccggc taacgtatcc acgccgtatt cggtgatgat
    12481 aatcggctga tgcagtttct cctgccaggc cagaagttct ttttccagta ccttctctgc
    12541 cgtttccaaa tcgccgcttt ggacatacca tccgtaataa cggttcaggc acagcacatc
    12601 aaagagatcg ctgatggtat cggtgtgagc gtcgcagaac attacattga cgcaggtgat
    12661 cggacgcgtc gggtcgagtt tacgcgttgc ttccgccagt ggcgcgaaat attcccgtgc
    12721 accttgcgga cgggtatccg gttcgttggc aatactccac atcaccacgc ttgggtggtt
    12781 tttgtcacgc gctatcagct ctttaatcgc ctgtaagtgc gcttgctgag tttccccgtt
    12841 gactgcctct tcgctgtaca gttctttcgg cttgttgccc gcttcgaaac caatgcctaa
    12901 agagaggtta aagccgacag cagcagtttc atcaatcacc acgatgccat gttcatctgc
    12961 ccagtcgagc atctcttcag cgtaagggta atgcgaggta cggtaggagt tggccccaat
    13021 ccagtccatt aatgcgtggt cgtgcaccat cagcacgtta tcgaatcctt tgccacgcaa
    13081 gtccgcatct tcatgacgac caaagccagt aaagtagaac ggtttgtggt taatcaggaa
    13141 ctgttcgccc ttcactgcca ctgaccggat gccgacgcga agcgggtaga tatcacactc
    13201 tgtctggctt ttggctgtga cgcacagttc atagagataa ccttcacccg gttgccagag
    13261 gtgcggattc accacttgca aagtcccgct agtgccttgt ccagttgcaa ccacctgttg
    13321 atccgcatca cgcagttcaa cgctgacatc accattggcc accacctgcc agtcaacaga
    13381 cgcgtggtta cagtcttgcg cgacatgcgt caccacggtg atatcgtcca cccaggtgtt
    13441 cggcgtggtg tagagcatta cgctgcgatg gattccggca tagttaaaga aatcatggaa
    13501 gtaagactgc tttttcttgc cgttttcgtc ggtaatcacc attcccggcg ggatagtctg
    13561 ccagttcagt tcgttgttca cacaaacggt gatacgtaca cttttcccgg caataacata
    13621 cggcgtgaca tcggcttcaa atggcgtata gccgccctga tgctccatca cttcctgatt
    13681 attgacccac actttgccgt aatgagtgac cgcatcgaaa cgcagcacga tacgctggcc
    13741 tgcccaacct ttcggtataa agacttcgcg ctgataccag acgttgcccg cataattacg
    13801 aatatctgca tcggcgaact gatcgttaaa actgcctggc acagcaattg cccggctttc
    13861 ttgtaacgcg ctttcccacc aacgctgatc aattccacag ttttcgcgat ccagactgaa
    13921 tgcccacagg ccgtcgagtt ttttgatttc acgggttggg gtttctacag gacggacgag
    13981 tcgtcggttc tgtaactatc atcatcatca tagacacacg aaataaagta atcagattat
    14041 cagttaaagc tatgtaatat ttacaccata accaatcaat taaaaaatag atcagtttaa
    14101 agaaagatca aagctcaaaa aaataaaaag agaaaagggt cctaaccaag aaaatgaagg
    14161 agaaaaacta gaaatttacc ctcagatcta ccatggtcaa gagtcccccg tgttctctcc
    14221 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
    14281 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
    14341 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
    14401 gcagaggcat cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca
    14461 ccttcctttt ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg
    14521 aggtttccgg atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact
    14581 gtatctttga tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt gacgaagatt
    14641 ttcttcttgt cattgagtcg taagagactc tgtatgaact gttcgccagt ctttacggcg
    14701 agttctgtta ggtcctctat ttgaatcttt gactccatga agctaaactg aaggcgggaa
    14761 acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct gcgcaactgt
    14821 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    14881 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    14941 acggccagtg ccaagcttgc atgcctgcag
//
