LOCUS       sequence-174447-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP7 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP7.v2 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    886..907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(922..927,928..945,946..952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    960..976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     965..987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     984..1000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     984..1000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1019..1039
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1021..1039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1067..1293
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1294..1474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1521..1646
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2139..2372
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2557..2601
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2890..2984
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2901..2920
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2966..2984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3085..3202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3251..3761
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3680..3698
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3770..4342
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4350..4369
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4397..4415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4426..5046
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4499..4518)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5081..5654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5248..5265)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5475..5494
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5674..6273
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5674..5693)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6170..6190
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6401..6634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6706..6827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6743..6762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6797..6816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6867..7002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7023..7041)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7051..7068)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7051..7067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7060..7082)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7209..7664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7296..7315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7506..7527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7690..7794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7795..7863,7864..8655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8013..8032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      901 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      961 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     1021 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     1081 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     1141 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     1201 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     1261 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     1321 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1381 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1441 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1501 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1561 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1621 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1681 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1741 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1801 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1861 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1921 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1981 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     2041 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     2101 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     2161 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     2221 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     2281 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     2341 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     2401 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2461 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2521 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2581 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2641 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2701 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2761 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2821 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2881 gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatcc
     2941 ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg gcaacaggac
     3001 aaagtgttaa tctgcagatt aacactttgt cctgttgctt tttgaattct cgacctcgag
     3061 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3121 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3181 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     3241 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     3301 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     3361 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     3421 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     3481 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     3541 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     3601 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     3661 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     3721 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatctg tgagtttggg
     3781 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
     3841 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
     3901 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
     3961 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
     4021 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
     4081 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
     4141 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
     4201 aaacaactac atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
     4261 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
     4321 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     4381 ttttggcaaa gaattgtaat acgactcact atagggcgaa ttgatatgtc tagattagat
     4441 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
     4501 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
     4561 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
     4621 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
     4681 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
     4741 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
     4801 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
     4861 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
     4921 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
     4981 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
     5041 gggtccgcgt acagcggctc ccgggagttc tagggatctg cccctctccc tccccccccc
     5101 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5161 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5221 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5281 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5341 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5401 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5461 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5521 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5581 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5641 aaacacgatg ataaggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc
     5701 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5761 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     5821 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5881 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5941 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6001 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6061 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6121 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6181 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6241 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     6301 accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca
     6361 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6421 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6601 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6661 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6721 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6781 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6841 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6901 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6961 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7021 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7081 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7141 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7201 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7621 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7681 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7801 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7861 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7921 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7981 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8041 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8101 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8161 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8221 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8281 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8341 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8401 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8461 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8521 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8581 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8641 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8701 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8761 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaa
//
