LOCUS       sequence-174448-       13385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR393 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 13385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM393
FEATURES             Location/Qualifiers
     source          1..13385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        239..584
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    745..921
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1483..1501)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1484..1501)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2140..2159
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2262..2267,2268..2284,2285..2290))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2625..2671
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(2690..2704,2895..4712)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          2705..2894
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4719..4736
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4771..5023
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5045..5069
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6370..6999
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7428..8501
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8567..8761
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8997..9019)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9105..9245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9160..9179
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9260..9277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9431..10019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10106..10900)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11325..11349
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11427..11601
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11642..12667)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12740..13085)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
       61 gtggtcccaa agatggaccc ccacccacga ggagcatcgt gggaaaagaa gacgttccaa
      121 ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
      181 aatcccaccc ctactccaaa aatgtcaaag atactgtctc agaagaccaa agggctattg
      241 agacttttca acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct
      301 gtcacctcat cgaaaggaca gtagaaaagg aaggtggccc ctacaaatgc catcattgcg
      361 ataaaggaaa ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc
      421 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
      481 attgatgtga catctccact gacataaggg atgacgcaca accccactat ccttcgcaag
      541 acccttcctc tatataagga agttcatttc atttggagag gacagcccaa gcttgatcaa
      601 tgcgactatc cctttggagt tgttgttgtt atggtctaat ttaaatatgg tctaaagaag
      661 aagaatgatc aatgcgacta tccctttgga gaattcggta cgctgaaatc accagtctct
      721 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
      781 agggaaatta gggttcttat agggtttcgc tcatgtgtcg agcatataag aaacccttag
      841 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
      901 tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg aatataaacc
      961 agacacgaga cgactagacc tggagcccag acgccattcg aagctagaag taccgcttag
     1021 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
     1081 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
     1141 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
     1201 actatacgct aagggaatga ttgtatttat accctatacc ccctaataac cccttatcaa
     1261 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
     1321 ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtc cctaactcta
     1381 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
     1441 gcgacctcta gaccatcaca ttggagcttc agctgctcga gttctatagt gtcacctaaa
     1501 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttctt acgtcgacgt
     1561 accaattacg ccccgccctg tcactcatcg cagtactgtt gtaattcatt aagcattctg
     1621 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1681 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1741 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1801 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1861 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1921 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatattacc
     1981 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     2041 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2101 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2161 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttctttc
     2221 tccattttag cttccttagc tcctgaaaat ctcgataact cattaaagct tatcgatgat
     2281 aagctgtcaa acatgagaat ttagcccgcc taatgagcgg gctttttgtt gtgatccgcg
     2341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cccctggaag
     2461 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2521 taattaactg caggcatgca agcttccttt tgtctccctt ttgtctccct tttgtctccc
     2581 ttttgtctcc cttttgtctc ccttttgtct cccttttgtc tctcgcaaga cccttcctct
     2641 atataaggaa gttcatttca tttggagaga acacggggga ctcttgacca tggtagatct
     2701 gagggtaaat ttctagtttt tctccttcat tttcttggtt aggacccttt tctcttttta
     2761 tttttttgag ctttgatctt tctttaaact gatctatttt ttaattgatt ggttatggtg
     2821 taaatattac atagctttaa ctgataatct gattacttta tttcgtgtgt ctatgatgat
     2881 gatgatagtt acagaaccga cgactcgtcc gtcctgtaga aaccccaacc cgtgaaatca
     2941 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
     3001 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
     3061 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
     3121 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
     3181 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
     3241 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
     3301 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
     3361 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
     3421 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
     3481 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
     3541 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
     3601 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     3661 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
     3721 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
     3781 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
     3841 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     3901 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     3961 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     4021 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     4081 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     4141 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     4201 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     4261 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     4321 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     4381 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     4441 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     4501 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     4561 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     4621 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     4681 tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt
     4741 gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     4801 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4861 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     4921 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     4981 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag
     5041 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     5101 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     5161 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     5221 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     5281 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     5341 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     5401 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     5461 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     5521 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     5581 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     5641 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     5701 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     5761 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     5821 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     5881 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     5941 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     6001 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     6061 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     6121 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     6181 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     6241 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     6301 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     6361 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     6421 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     6481 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     6541 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     6601 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     6661 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     6721 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     6781 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     6841 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     6901 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     6961 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     7021 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     7081 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     7141 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     7201 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     7261 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     7321 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
     7381 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     7441 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     7501 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     7561 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     7621 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     7681 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     7741 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     7801 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     7861 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     7921 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     7981 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     8041 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     8101 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     8161 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     8221 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     8281 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     8341 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     8401 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     8461 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     8521 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     8581 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     8641 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     8701 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     8761 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     8821 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     8881 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     8941 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9001 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     9061 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     9121 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     9181 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9241 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9841 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     9901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10081 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
    10141 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    10201 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    10261 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    10321 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    10381 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    10441 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    10501 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    10561 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    10621 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    10681 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    10741 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    10801 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    10861 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    10921 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    10981 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    11041 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
    11101 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    11161 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    11221 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    11281 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    11341 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    11401 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg
    11461 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
    11521 atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac
    11581 tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt cggcatctac
    11641 tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
    11701 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
    11761 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
    11821 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
    11881 ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
    11941 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
    12001 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
    12061 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
    12121 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
    12181 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
    12241 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
    12301 cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca
    12361 ggtcttgcaa cgtgacaccc tgtgaacggc gggagatgca ataggtcagg ctctcgctaa
    12421 actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
    12481 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
    12541 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
    12601 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
    12661 ttttcatatc tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga
    12721 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
    12781 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    12841 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    12901 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    12961 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    13021 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    13081 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    13141 tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    13201 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    13261 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    13321 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    13381 cccga
//
