LOCUS       sequence-174454-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human DUSP19 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shDUSP19 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     529..548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     782..799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    916..937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(952..957,958..975,976..982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    990..1006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     995..1017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1014..1030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1014..1030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1049..1069
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1051..1069
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1097..1323
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1324..1504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1551..1676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2169..2402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2587..2631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2920..3014
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2931..2950
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2968..2986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2996..3014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3115..3232
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3281..3791
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3710..3728
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3800..4372
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4380..4399
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4427..4445
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4456..5076
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4529..4548)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5111..5684
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5278..5295)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5505..5524
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5704..6303
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5704..5723)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6200..6220
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6431..6664
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6736..6857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6773..6792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6827..6846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6897..7032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7053..7071)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7081..7098)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7081..7097)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7090..7112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7239..7694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7326..7345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7536..7557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7720..7824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7825..7893,7894..8685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8043..8062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       61 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      181 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      721 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      781 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
      841 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
      901 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
      961 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1021 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     1081 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     1141 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     1201 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     1261 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     1321 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1501 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1561 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1621 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1681 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1741 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1801 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1861 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1921 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1981 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     2041 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     2101 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     2161 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     2221 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2281 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2341 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2401 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2461 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2521 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2581 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2641 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2701 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2761 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2821 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2881 cgacggtatc gatcacgaga ctagcctcga gcggccgcaa tatttgcatg tcgctatgtg
     2941 ttctgggaaa tcaccataaa cgtgaaatcc ctatcagtga tagagactta taagttccct
     3001 atcagtgata gagacaccgg atggagcagc ttcgtacata tctgcagata tgtacgaagc
     3061 tgctccattt tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     3121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3181 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     3241 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
     3301 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     3361 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     3421 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     3481 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     3541 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     3601 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     3661 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     3721 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     3781 tctctcccca gggggatctg tgagtttggg gacccttgat tgttctttct ttttcgctat
     3841 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     3901 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     3961 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     4021 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     4081 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     4141 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     4201 tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc
     4261 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     4321 caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     4381 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact
     4441 atagggcgaa ttgatatgtc tagattagat aaaagtaaag tgattaacag cgcattagag
     4501 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     4561 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     4621 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     4681 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     4741 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     4801 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     4861 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     4921 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     4981 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     5041 ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagcggctc ccgggagttc
     5101 tagggatctg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     5161 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5221 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5281 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5341 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5401 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     5461 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5521 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     5581 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     5641 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc accggagctt
     5701 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5761 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5821 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5881 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5941 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6001 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6061 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6121 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6181 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6241 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6301 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     6361 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6421 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6481 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6541 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6601 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6661 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6721 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6841 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6901 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6961 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7021 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     7081 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7141 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7201 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     7261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7441 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7561 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7681 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttcca
//
