LOCUS       sequence-174455-       14828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR156 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 14828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Osa-pCAMBIA-1301-STTM156
FEATURES             Location/Qualifiers
     source          1..14828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    191..212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(227..232,233..250,251..257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     270..292
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     289..305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     289..305
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     504..523
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        504..522
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        930..1275
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1452..1628
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2190..2208)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2191..2208)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(2279..2938)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFGAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(2975..2980,2981..2997,2998..3003))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3262..3279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3262..3278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3271..3293)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3655..4000
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(4017..4031,4222..6039)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          4032..4221
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             6046..6063
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6098..6350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6372..6396
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7697..8326
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8755..9828
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9894..10088
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10324..10346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10432..10572
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10487..10506
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10587..10604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10758..11346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11433..12227)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12652..12676
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12754..12928
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12969..13994)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14061..14737)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
       61 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      301 atgacatgat tacgaattcg agctcggtac ccggggatcc tctagctaga ttaattaagt
      361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa ggagatcctt tggatcacaa
      421 aaaaaagccc actcattagg cgggttaaat tcccgggtac tgcagccgat tcattaatgc
      481 aggttaacct ggcttatcga aattaatacg actcactata gggagaccgg tagatctgat
      541 ccctactcca aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt
      601 caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      661 atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      721 aaggctatca ttcaagatgc ctctgccgac agtggtccca gagatggacc cccacccacg
      781 aggagcatcg tgggaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
      841 gacatctcca ctgacgtaag gggtgacgca caatcccgcc cctactccaa aaatgtcaaa
      901 gatactgtct cagaagacca aagggctatt gagacttttc aacaaagggt aatttcggga
      961 aacctcctcg gattccattg cccagctacc tgtcacctca tcgaaaggac agtagaaaag
     1021 gaaggtggct cctacaaatg ccatcatcgc gataaaggaa aggctatcat tcaagatgcc
     1081 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     1141 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacataagg
     1201 gatgacgcac aaccccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     1261 catttggaga ggacagccca agcttggtac cgtgctcact cgagtcttct gtcagttgtt
     1321 gttgttatgg tctaatttaa atatggtcta aagaagaaga atgtgctcac tcgagtcttc
     1381 tgtcaggatc cactagtgaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta
     1441 tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg
     1501 ttcttatagg gtttcgctca tgtgttgagc atataagaga cccttagtat gtatttgtat
     1561 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     1621 tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga
     1681 ctagacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt
     1741 agggaaaaga tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat
     1801 atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg
     1861 caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag
     1921 ggaatgattg tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat
     1981 ccgcataagc ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac
     2041 tcaagaggat aaaacctcac caaaatacga aagagtcctt aactctaaag ataaaagatc
     2101 tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg acctctagac
     2161 catcacattg gagcttcagc tgctcgagtt ctatagtgtc acctaaatca tatgtgtatg
     2221 atacataagg ttatgtatta attgtagccg cgttctaacg tcgacaggcc tgtaccaatt
     2281 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2341 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2401 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2461 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2521 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2581 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2641 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatt accagctcac
     2701 cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa
     2761 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2821 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc ccaaaatgtt
     2881 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgatttct ttctccattt
     2941 tagcttcctt agctcctgaa aatctcgata actcattaaa acttatcgat gataagctgt
     3001 caaacatgag aatttagccc gcctaatgag cgggcttttt gctgtgatcc gcgttgctgg
     3061 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccccctgg aagctccctc
     3181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tcttaattaa
     3241 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3301 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3361 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     3421 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttagct tcatggagtc
     3481 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     3541 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     3601 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     3661 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     3721 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     3781 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     3841 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     3901 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     3961 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc ttgaccatgg
     4021 tagatctgag ggtaaatttc tagtttttct ccttcatttt cttggttagg acccttttct
     4081 ctttttattt ttttgagctt tgatctttct ttaaactgat ctatttttta attgattggt
     4141 tatggtgtaa atattacata gctttaactg ataatctgat tactttattt cgtgtgtcta
     4201 tgatgatgat gatagttaca gaaccgacga ctcgtccgtc ctgtagaaac cccaacccgt
     4261 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     4321 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     4381 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
     4441 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     4501 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     4561 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     4621 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     4681 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     4741 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     4801 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     4861 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     4921 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     4981 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     5041 atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt
     5101 actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg
     5161 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     5221 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     5281 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     5341 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     5401 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     5461 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa
     5521 gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
     5581 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga
     5641 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
     5701 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
     5761 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
     5821 gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
     5881 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc
     5941 ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
     6001 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaag ctagccacca ccaccaccac
     6061 cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata
     6121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     6181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     6241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     6301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa
     6361 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     6421 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     6481 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     6541 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     6601 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     6661 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     6721 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     6781 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     6841 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     6901 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     6961 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     7021 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     7081 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     7141 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     7201 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     7261 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     7321 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     7381 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     7441 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     7501 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     7561 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     7621 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     7681 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     7741 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     7801 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     7861 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     7921 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     7981 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     8041 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     8101 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     8161 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     8221 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     8281 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     8341 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     8401 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     8461 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     8521 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     8581 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     8641 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     8701 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     8761 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     8821 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     8881 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     8941 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     9001 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     9061 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     9121 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     9181 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     9241 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     9301 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     9361 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     9421 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     9481 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     9541 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     9601 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     9661 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     9721 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     9781 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     9841 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     9901 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     9961 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
    10021 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
    10081 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
    10141 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
    10201 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    10261 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    10321 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10381 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10441 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10501 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10561 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10621 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10681 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11161 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    11221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11341 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11401 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
    11461 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11521 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11581 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11641 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11701 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    11761 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    11821 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    11881 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    11941 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12001 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12061 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12121 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12181 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12241 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12301 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12361 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    12421 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    12481 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    12541 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    12601 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    12661 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    12721 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
    12781 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
    12841 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
    12901 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
    12961 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
    13021 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
    13081 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
    13141 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
    13201 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
    13261 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
    13321 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
    13381 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
    13441 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
    13501 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
    13561 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
    13621 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
    13681 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
    13741 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
    13801 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
    13861 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
    13921 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
    13981 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
    14041 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    14101 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
    14161 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    14221 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    14281 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    14341 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    14401 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    14461 acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg
    14521 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    14581 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    14641 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    14701 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    14761 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
    14821 ctctagcc
//
