LOCUS       sequence-174476-        2044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-<FRT.STOP<Bxb1
FEATURES             Location/Qualifiers
     source          1..2044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    221..355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(258..277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     312..331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    356..389
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
       61 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat
      121 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      181 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgaagt
      361 tcctattctc tagaaagtat aggaacttcc aaaatgcgag ccctggtcgt catccgtttg
      421 tcccgtgtca ccgatgctac gacgtccccg gagcgtcagc tggagtcgtg ccagcagctc
      481 tgcgcccagc gcggctggga tgtcgtcgga gtggctgagg atctggatgt ctccggagct
      541 gtcgatccgt tcgatcgtaa gcgccgtcca aacctggccc gttggctggc tttcgaggag
      601 caaccgttcg atgtgatcgt ggcttaccgt gtcgaccgtt tgacccgatc gattcgtcat
      661 ctgcagcagc tggtccactg ggctgaggat cacaagaagt tggtcgtctc cgctaccgaa
      721 gctcacttcg atacgaccac gccattcgct gctgtcgtga tcgctctcat gggaacggtg
      781 gctcagatgg aattggaggc tatcaaagag cgtaaccgta gcgctgccca tttcaacatt
      841 cgtgccggaa agtaccgtgg aagcttgcca ccgtggggtt acttgccaac gcgtgtggat
      901 ggagagtggc gtctgttggt cgatccggtg cagcgtgagc gtattctgga ggtctaccac
      961 cgtgtcgtgg ataaccacga gccgttgcac ttggtggctc acgatttgaa ccagcgtgga
     1021 attctgagcc cgaaggatta cttcgctcag ttgcagggac gtgagccgca gggacgtgct
     1081 tggtcggcta ccgccttgaa gcgtagcttg atctccgagg ctatgttggg atacgctacc
     1141 ttgaacggaa agaccgtgcg tgatgatgat ggagctccgt tggtgcgtgc tgagccgatc
     1201 ttgacgcgtg agcagttgga ggctctgcgc gccgagttgg tgaagaccga tcgtaccaag
     1261 ccggctgtga gcaccccgag cctgttgctg cgtgtgttgt tctgcgctgt gtgcggagag
     1321 cccgcttaca agttcgctgg aggaggccgt aagcacccgc gttaccgttg ccgttccatg
     1381 ggattcccga agcactgcgg aaacggaacc gtcgctatgg ccgagtggga tgctttctgc
     1441 gaggagcagg tgctggatct gttgggagac gctgagcgtc tggagaaggt ctgggtcgct
     1501 ggctccgatg ctgccgtgga gttggctgag ttgaacgctg agttggtgga tttgacgagc
     1561 ttgattggaa gcccggctta ccgcgccggc agcccgcagc gtgaggctct ggatgctcgt
     1621 atcgaggctc tggccgctcg tcaggaggag ttggagggac tggaggctcg cccgtccgga
     1681 tgggagtggc gtgagaccgg acagcgtttc ggagattggt ggcgtgagca ggataccgct
     1741 gctaagaaca cctggttgcg tagcatgaac gtgcgtctga ccttcgatgt ccgtggagga
     1801 ttgacccgta ccatcgattt cggagatctg caggagtacg agcagcactt gcgtctgggc
     1861 agcgtggtcg agcgtttgca caccggaatg agctaagtac aaagtggtgg gtaccggatc
     1921 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1981 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     2041 attg
//
