LOCUS       sequence-174504-        7745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-<FRT.STOP<Bxb1
FEATURES             Location/Qualifiers
     source          1..7745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(52..70)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        137..241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(242..310,311..1102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(460..479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1273..1861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1762..1781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1948..2017
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2242..2260)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2712..2783,3185..3458,3533..4187,4249..4564,4768..4899,
                     4970..5584)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6614..6708
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6742..6836
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6867..7105
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7052..7073
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    7127..7151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7174..7207
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7501..7517)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7502..7518)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          7609..7674
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tgtctggatc gatctggccg gccgtttaaa cgaattcttg aagacgaaag ggcctcgtga
       61 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagact caggtggcac
      121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      481 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      541 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      781 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      841 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      901 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      961 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1021 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1081 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1381 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1921 gttaccgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
     1981 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
     2041 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
     2101 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga
     2161 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga caagccgaac
     2221 atatgggcgc gcctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata
     2281 aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta
     2341 aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa
     2401 aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc
     2461 cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg
     2521 tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt
     2581 acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt
     2641 gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa
     2701 aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc
     2761 catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg
     2821 atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca
     2881 aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc
     2941 tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat
     3001 tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc
     3061 ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact
     3121 ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt
     3181 gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa
     3241 aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca
     3301 actagccgag aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca
     3361 gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg
     3421 acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg
     3481 ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc
     3541 gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc
     3601 ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg
     3661 cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc
     3721 cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc
     3781 atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg
     3841 atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg
     3901 aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat
     3961 ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc
     4021 gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat
     4081 cagccgtctt ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg
     4141 gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt
     4201 gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca
     4261 gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg
     4321 acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa
     4381 agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca
     4441 gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg
     4501 gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac
     4561 aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat
     4621 tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga
     4681 tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg
     4741 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     4801 ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg
     4861 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca
     4921 tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     4981 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     5041 tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc
     5101 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     5161 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     5221 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     5281 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     5341 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     5401 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     5461 ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     5521 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     5581 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     5641 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     5701 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     5761 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     5821 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     5881 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     5941 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     6001 gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa
     6061 taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
     6121 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
     6181 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
     6241 catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat
     6301 aataataaga atacatttaa tttagaaaat gcttggattt cactggaact agggcgcgcc
     6361 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     6421 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     6481 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     6541 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccagggc cgcaagcttg
     6601 catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6661 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactctag
     6721 ccctagggca tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc
     6781 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     6841 gactctagcg ctagcgacgt cgagcgccgg agtataaata gaggcgcttc gtctacggag
     6901 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     6961 acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat
     7021 taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat
     7081 tattgaatac aagaagagaa ctctgaatgc ggccgcccag atatcgacaa gtttgtacaa
     7141 aaaagcaggc tggactctag ctagaggatc ccggaagttc ctattctcta gaaagtatag
     7201 gaacttcgaa tgactaaagc caaatagaaa attattcagt tcctggctta agtttttaaa
     7261 agtgatatta tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt
     7321 atgttagttt taagttagca acaaattgat tttagctata ttagctactt ggttaataaa
     7381 tagaatatat ttatttaaag ataattgcgt ttttattgtc agggagtgag tttgcttaaa
     7441 aactcgttta gatccactag ttctagttgc ggccggctaa gaattcgata tcaagcttat
     7501 cgataccgtc gacctcgagg gtacctctag aggatctttg tgaaggaacc ttacttctgt
     7561 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     7621 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     7681 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     7741 tgctc
//
