LOCUS       sequence-174518-       11178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            UAS->Bxb1.STOP>myr::4xSNAPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS->Bxb1.STOP>myr::4xSNAPf
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6508..6602
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6636..6730
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6761..6996
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6943..6964
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    7278..7325
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             7330..7419
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(7443..7459)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7444..7460)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          7551..7616
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7746..7766
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8038..8172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8075..8094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8129..8148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8174..8211
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             8215..8760
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             8779..9324
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             9343..9888
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             9910..10455
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg
     6781 cttcgtctac ggagcgacaa tattcaaaca agcaaagtga acacgtcgct aagcgaaagc
     6841 taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa
     6901 agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc
     6961 caagaagtaa ttattgaata caagaagaga actctgaata attacaaatt atataacaaa
     7021 atgggcaaca aatgctgcag caagcgacag gatcaggaac tggcactggc ctatcccact
     7081 gggggctaca agaaatccga ctacaccttt ggccagacgc acatcaacag cagcggcggc
     7141 ggcaacatgg gcggcgttct tggccagaag cataacaacg gtggctcgct ggactcgcgc
     7201 tacacgcccg atcccaatca tcggggtccg ttgaaaatcg gcggaaaggg cggcgttgac
     7261 atcatcagac cacgcgtggt ttgtctggtc aaccaccgcg gtctcagtgg tgtacggtac
     7321 aaacccagtt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     7381 tatgcaggat cctatccata tgacgttcca gattacgctt aagaattcga tatcaagctt
     7441 atcgataccg tcgacctcga gggtacctct agaggatctt tgtgaaggaa ccttacttct
     7501 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     7561 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     7621 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     7681 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     7741 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     7801 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     7861 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg
     7921 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7981 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8161 catgtctgga tccggcttgt cgacgacggc ggtctccgtc gtcaggatca tcttatggac
     8221 aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct ggaactgtct
     8281 gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc tgccgccgac
     8341 gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact gatgcaggcc
     8401 accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca
     8461 gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct gtggaaactg
     8521 ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctcgctgc cctcgctggc
     8581 aatccagctg ctacagccgc cgttaaaacc gccctgagcg gaaatcccgt gcccattctg
     8641 atcccctgcc accgggtggt gcagggcgac ctggacgtgg gaggctacga gggcgggctc
     8701 gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc tgggctgggt
     8761 agcgtaagcg gtagtacaat ggacaaagac tgcgaaatga agcgcaccac cctggatagc
     8821 cctctgggca agctggaact gtctgggtgc gaacagggcc tgcaccgtat catcttcctg
     8881 ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc
     8941 ggaccagagc cactgatgca ggccaccgcc tggctcaacg cctactttca ccagcctgag
     9001 gccatcgagg agttccctgt gccagccctg caccacccag tgttccagca ggagagcttt
     9061 acccgccagg tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt catcagctac
     9121 agccacctcg ctgccctcgc cggcaatcca gctgctacag ccgccgttaa aaccgccctg
     9181 agcggaaatc ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg cgacctggac
     9241 gtgggaggct acgagggcgg gctcgccgtg aaagagtggc tgctggccca cgagggccac
     9301 agactgggca agcctgggct gggtgtaagc ggtagcagta ccatggacaa agactgcgaa
     9361 atgaagcgca ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag
     9421 ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg
     9481 cctgccccag ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc
     9541 aacgcctact ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac
     9601 ccagtgttcc agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg
     9661 aagttcggag aggtcatcag ctacagccac ctcgctgccc tcgccggcaa tccagctgct
     9721 acagccgccg ttaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac
     9781 cgggtggtgc agggcgacct ggacgtggga ggctacgagg gcgggctcgc cgtgaaagag
     9841 tggctgctgg cccacgaggg ccacagactg ggcaagcctg ggctgggttc ggttggtagt
     9901 agtagcacga tggacaaaga ctgcgaaatg aagcgcacca ccctggatag ccctctgggc
     9961 aagctggaac tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct gggcaaagga
    10021 acatctgccg ccgacgccgt ggaagtgcct gccccagccg ccgtgctggg cggaccagag
    10081 ccactgatgc aggccaccgc ctggctcaac gcctactttc accagcctga ggccatcgag
    10141 gagttccctg tgccagccct gcaccaccca gtgttccagc aggagagctt tacccgccag
    10201 gtgctgtgga aactgctgaa agtggtgaag ttcggagagg tcatcagcta cagccacctc
    10261 gctgccctcg ccggcaatcc agctgctaca gccgccgtta aaaccgccct gagcggaaat
    10321 cccgtgccca ttctgatccc ctgccaccgg gtggtgcagg gcgacctgga cgtgggaggc
    10381 tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc acgagggcca cagactgggc
    10441 aagcctgggc tgggttagtg actcgagact agttaatcta gaatgaatcg tttttaaaat
    10501 aacaaatcaa ttgttttata atattcgtac gattctttga ttatgtaata aaatgtgatc
    10561 attaggaaga ttacgaaaaa tataaaaaat atgagttctg tgtgtataac aaatgctgta
    10621 aacgccacaa ttgtgtttgt tgcaaataaa cccatgatta tttgattaaa attgttgttt
    10681 tctttgttca tagacaatag tgtgttttgc ctaaacgtgt actgcataaa ctccatgcga
    10741 gtgtatagcg agctagtggc taacgcttgc cccaccaaag tagattcgtc aaaatcctca
    10801 atttcatcac cctcctccaa gtttaacatt tggccgtcgg aattaacttc taaagatgcc
    10861 acataatcta ataaatgaaa tagagattca aacgtggcgt catcgtccgt ttcgaccatt
    10921 tccgaaaaga actcgggcat aaactctatg atttctctgg acgtggtgtt gtcgaaactc
    10981 tcaaagtacg cagtcaggaa cgtgcgcgac atgtcgtcgg gaaactcgcg cggaaacatg
    11041 ttgttgtaac cgaacgggtc ccatagcgcc aaaaccaaat ctgccagcgt caatagaatg
    11101 agcacgatgc cgacaatgga gctggcttgg atagcgattc gagttaacgg ttaatgtcat
    11161 gataataatg gtttctta
//
