LOCUS       sequence-17453-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Devroe E, Silver PA
  TITLE     Retrovirus-delivered siRNA.
  JOURNAL   BMC Biotechnol. 2002 Aug 28;2:15. doi: 10.1186/1472-6750-2-15.
  PUBMED    12199908
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2002-08-28"; volume: "2:15. doi"; pages: " 
            10.1186/1472-6750-2-15"
COMMENT     Sequence Label: pPS2971
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..46
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        43..356
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     275..295
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(430..446)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(483..503)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(483..501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(522..538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(535..557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    546..562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(570..576,577..594,595..600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    615..636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actcactata gggcgaattg ggtacccgct ctagaactag tggatccgac gccgccatct
       61 ctaggcccgc gccggccccc tcgcacagac ttgtgggaga agctcggcta ctcccctgcc
      121 ccggttaatt tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga
      181 cagataatct gttcttttta atactagcta cattttacat gataggcttg gatttctata
      241 agagatacaa atactaaatt attattttaa aaaacagcac aaaaggaaac tcaccctaac
      301 tgtaaagtaa ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgtttggg
      361 acatgatgac cttgttgaaa gctttcaaca aggtcatcat gtcccttttt gaattcctgc
      421 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
      481 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
      541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tcaggggata acgcaggaaa gaacatgtga gcaaaaagcc agcaaaaggc caggaac
//
