LOCUS       sequence-174553-        7786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: LexAop2-myr::4xSNAPf
FEATURES             Location/Qualifiers
     source          1..7786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(60..78)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        145..249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(250..318,319..1110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(468..487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1281..1869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1770..1789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1956..2025
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2250..2268)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2720..2791,3193..3466,3541..4195,4257..4572,4776..4907,
                     4978..5592)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        7004..7242
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7189..7210
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7257..7324
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 cgatgccgac aatggagctg gcttggatag cgattcgagt taaccttgaa gacgaaaggg
       61 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagactca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      241 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      301 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      361 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      421 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      481 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      541 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      601 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      661 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      721 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      781 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      841 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      901 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      961 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1021 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1081 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1141 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1201 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1261 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1321 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1381 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1441 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1501 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1561 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1621 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1681 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1741 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1801 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1861 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1921 gctcacatgt taccgtcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag
     1981 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
     2041 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
     2101 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg
     2161 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
     2221 agccgaacat atgggcgcgc ctagtatgta tgtaagttaa taaaacccat ttttgcggaa
     2281 agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat
     2341 cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc
     2401 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
     2461 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
     2521 acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca
     2581 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
     2641 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
     2701 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
     2761 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
     2821 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
     2881 gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt
     2941 ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct
     3001 aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta
     3061 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     3121 ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc
     3181 ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg
     3241 caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg
     3301 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     3361 gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     3421 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     3481 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     3541 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     3601 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     3661 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     3721 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt
     3781 tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg
     3841 atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg
     3901 gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc
     3961 taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg
     4021 cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga
     4081 ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg
     4141 agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag
     4201 ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg
     4261 ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt
     4321 gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc
     4381 attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca
     4441 ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg
     4501 gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt
     4561 attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg
     4621 aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt
     4681 caatgagatg tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa
     4741 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
     4801 tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc
     4861 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt
     4921 agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg
     4981 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
     5041 acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc
     5101 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
     5161 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
     5221 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
     5281 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
     5341 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
     5401 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
     5461 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
     5521 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
     5581 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt
     5641 accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa
     5701 taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta
     5761 gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     5821 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     5881 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     5941 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     6001 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     6061 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     6121 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     6181 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     6241 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     6301 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     6361 ggcgcgcctc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga
     6421 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
     6481 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg
     6541 ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccagggccg
     6601 caagcttgca tgcctgcagg ttactgtaca tccatacagt aagtactgta catccataca
     6661 gtaagtactg tacatccata cagtaagtac tgtacatcca tacagtaagt actgtacatc
     6721 catacagtaa gcggagactc tagccctagg gcatgcctgc aggttactgt acatccatac
     6781 agtaagtact gtacatccat acagtaagta ctgtacatcc atacagtaag cggagactct
     6841 agcgctagcg catgcctgca ggttactgta catccataca gtaagtactg tacatccata
     6901 cagtaagtac tgtacatcca tacagtaagt actgtacatc catacagtaa gtactgtaca
     6961 tccatacagt aagcggagac tctagcacta gtgacgtcga gcgccggagt ataaatagag
     7021 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     7081 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gtaaagtgca
     7141 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa
     7201 atctgccaag aagtaattat tgaatacaag aagagaactc tgaatagatc taaaaggtag
     7261 gttcaaccac tgatgcctag gcacaccgaa acgactaacc ctaatttctt atcctttact
     7321 tcaggcaatt acaaattata taacaaaatg ggcaacaaat gctgcagcaa gcgacaggat
     7381 caggaactgg cactggccta tcccactggg ggctacaaga aatccgacta cacctttggc
     7441 cagacgcaca tcaacagcag cggcggcggc aacatgggcg gcgttcttgg ccagaagcat
     7501 aacaacggtg gctcgctgga ctcgcgctac acgcccgatc ccaatcatcg gggtccgttg
     7561 aaaatcggcg gaaagggcgg cgttgacatc atcagaccac gcgtgatgga caaagactgc
     7621 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
     7681 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
     7741 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgc
//
