LOCUS       sequence-174554-       12452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
FEATURES             Location/Qualifiers
     source          1..12452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6508..6602
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6636..6730
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6761..6999
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6946..6967
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    7281..7328
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             7333..7422
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(7446..7462)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7447..7463)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          7554..7619
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7749..7769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8041..8175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8078..8097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8132..8151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8177..8214
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             8218..8763
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     protein_bind    9443..9480
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     intron          10038..10104
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    10800..10837
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg
     6781 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     6841 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
     6901 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
     6961 tgccaagaag taattattga atacaagaag agaactctga ataattacaa attatataac
     7021 aaaatgggca acaaatgctg cagcaagcga caggatcagg aactggcact ggcctatccc
     7081 actgggggct acaagaaatc cgactacacc tttggccaga cgcacatcaa cagcagcggc
     7141 ggcggcaaca tgggcggcgt tcttggccag aagcataaca acggtggctc gctggactcg
     7201 cgctacacgc ccgatcccaa tcatcggggt ccgttgaaaa tcggcggaaa gggcggcgtt
     7261 gacatcatca gaccacgcgt ggtttgtctg gtcaaccacc gcggtctcag tggtgtacgg
     7321 tacaaaccca gttacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg
     7381 gactatgcag gatcctatcc atatgacgtt ccagattacg cttaagaatt cgatatcaag
     7441 cttatcgata ccgtcgacct cgagggtacc tctagaggat ctttgtgaag gaaccttact
     7501 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     7561 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     7621 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     7681 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     7741 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     7801 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     7861 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat ttgatgtata
     7921 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     7981 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8041 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8101 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8161 tatcatgtct ggatccggct tgtcgacgac ggcggtctcc gtcgtcagga tcatcttatg
     8221 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
     8281 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
     8341 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
     8401 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
     8461 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
     8521 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctcgc tgccctcgct
     8581 ggcaatccag ctgctacagc cgccgttaaa accgccctga gcggaaatcc cgtgcccatt
     8641 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tgggaggcta cgagggcggg
     8701 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
     8761 ggttaaatga atctttaaaa taacaaatca attgttttat aatattcgta cgattctttg
     8821 attatgtaat aaaatgtgat cattaggaag attacgaaaa atataaaaaa tatgagttct
     8881 gtgtgtataa caaatgctgt aaacgccaca attgtgtttg ttgcaaataa acccatgatt
     8941 atttgattaa aattgttgtt ttctttgttc atagacaata gtgtgttttg cctaaacgtg
     9001 tactgcataa actccatgcg agtgtatagc gagctagtgg ctaacgcttg ccccaccaaa
     9061 gtagattcgt caaaatcctc aatttcatca ccctcctcca agtttaacat ttggccgtcg
     9121 gaattaactt ctaaagatgc cacataatct aataaatgaa atagagattc aaacgtggcg
     9181 tcatcgtccg tttcgaccat ttccgaaaag aactcgggca taaactctat gatttctctg
     9241 gacgtggtgt tgtcgaaact ctcaaagtac gcagtcagga acgtgcgcga catgtcgtcg
     9301 ggaaactcgc gcggaaacat gttgttgtaa ccgaacgggt cccatagcgc caaaaccaaa
     9361 tctgccagcg tcaatagaat gagcacgatg ccgacaatgg agctggcttg gatagcgatt
     9421 cgagttaaca aaaggtaggt tcggcttgtc gacgacggcg gtctccgtcg tcaggatcat
     9481 cttatggata aggattgcga gatgaagcgt acaacattgg attccccgtt gggaaagttg
     9541 gagttgtccg gatgcgagca gggattgcac cgcattatct tcttgggaaa gggcacctcc
     9601 gctgctgatg ctgtcgaggt ccccgctccg gctgctgtct tgggaggccc cgagcccttg
     9661 atccaggcta cggcttggtt gaacgcttac ttccaccagc ccgaggctat tgaggagttc
     9721 cccgtccccg ctttgcacca ccccgtcttc cagcaggagt ccttcacgcg tcaggtcttg
     9781 tggaagttgc tgaaggtcgt gaagttcggc gaggtgatct ccgagtccca cttggctgcc
     9841 ttggtgggaa acccggctgc cacggccgct gtcaacacgg ctttggatgg caaccccgtc
     9901 ccgatcttga tcccgtgcca ccgtgtggtc cagggagatt ccgatgtcgg accctacctg
     9961 ggaggcctgg ctgtcaagga gtggttgttg gctcacgagg gacaccgctt gggaaagccc
    10021 ggcttgggat gaaaaaggta ggttcaacca ctgatgccta ggcacaccga aacgactaac
    10081 cctaattctt atcctttact tcaggctcta gaatgaatcg tttttaaaat aacaaatcaa
    10141 ttgttttata atattcgtac gattctttga ttatgtaata aaatgtgatc attaggaaga
    10201 ttacgaaaaa tataaaaaat atgagttctg tgtgtataac aaatgctgta aacgccacaa
    10261 ttgtgtttgt tgcaaataaa cccatgatta tttgattaaa attgttgttt tctttgttca
    10321 tagacaatag tgtgttttgc ctaaacgtgt actgcataaa ctccatgcga gtgtatagcg
    10381 agctagtggc taacgcttgc cccaccaaag tagattcgtc aaaatcctca atttcatcac
    10441 cctcctccaa gtttaacatt tggccgtcgg aattaacttc taaagatgcc acataatcta
    10501 ataaatgaaa tagagattca aacgtggcgt catcgtccgt ttcgaccatt tccgaaaaga
    10561 actcgggcat aaactctatg atttctctgg acgtggtgtt gtcgaaactc tcaaagtacg
    10621 cagtcaggaa cgtgcgcgac atgtcgtcgg gaaactcgcg cggaaacatg ttgttgtaac
    10681 cgaacgggtc ccatagcgcc aaaaccaaat ctgccagcgt caatagaatg agcacgatgc
    10741 cgacaatgga gctggcttgg atagcgattc gagttaactt gaagacgaaa gggcctcgcg
    10801 gcttgtcgac gacggcggtc tccgtcgtca ggatcatctt atgggttccg aaatcggtac
    10861 aggcttcccc ttcgaccccc attatgtgga agtcctgggc gagcgtatgc actacgtcga
    10921 tgttggaccg cgggatggca cgcctgtgct gttcctgcac ggtaacccga cctcgtccta
    10981 cctgtggcgc aacatcatcc cgcatgtagc accgagtcat cggtgcattg ctccagacct
    11041 gatcgggatg ggaaaatcgg acaaaccaga cctcgattat ttcttcgacg accacgtccg
    11101 ctacctcgat gccttcatcg aagccttggg tttggaagag gtcgtcctgg tcatccacga
    11161 ctggggctca gctctcggat tccactgggc caagcgcaat ccggaacggg tcaaaggtat
    11221 tgcatgtatg gaattcatcc ggcctatccc gacgtgggac gaatggccag aattcgcccg
    11281 tgagaccttc caggccttcc ggaccgccga cgtcggccga gagttgatca tcgatcagaa
    11341 cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt ccgcttacgg aggtcgagat
    11401 ggaccactat cgcgagccct tcctcaagcc tgttgaccga gagccactgt ggcgattccc
    11461 caacgagctg cccatcgccg gtgagcccgc gaacatcgtc gcgctcgtcg aggcatacat
    11521 gaactggctg caccagtcac ctgtcccgaa gttgttgttc tggggcacac ccggcgtact
    11581 gatccccccg gccgaagccg cgagacttgc cgaaagcctc cccaactgca agacagtgga
    11641 catcggcccg ggattgttct tgctccagga agacaacccg gaccttatcg gcagtgagat
    11701 cgcgcgctgg ctccccgggc tggccggcta ggtacctcga gactagttaa tctagaatga
    11761 atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt
    11821 aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta
    11881 taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat
    11941 taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca
    12001 taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc aaagtagatt
    12061 cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg tcggaattaa
    12121 cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt
    12181 ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct ctggacgtgg
    12241 tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact
    12301 cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc aaatctgcca
    12361 gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg attcgagtta
    12421 acggttaatg tcatgataat aatggtttct ta
//
