LOCUS       sequence-17456-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Devroe E, Erdjument-Bromage H, Tempst P, Silver PA
  TITLE     Human Mob proteins regulate the NDR1 and NDR2 serine-threonine 
            kinases.
  JOURNAL   J Biol Chem. 2004 Jun 4. 279(23):24444-51.
  PUBMED    15067004
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jun 4. 279(23):24444-51."
COMMENT     Sequence Label: pPS2991
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggccga ttacaaggat gacgacgata
       61 agaattcgat ggcaatgacg gcagggacta caacaacctt tcctatgagc aaccataccc
      121 gggaaagagt gactgtagcc aagctcacat tggagaattt ttatagcaac ctaattttac
      181 agcatgaaga gagagaaacc aggcagaaga aattagaagt ggccatggaa gaagaaggat
      241 tagcagatga agagaaaaag ttacgtcgat cacaacacgc tcgcaaagaa acagagttct
      301 tacggctcaa aaggaccaga cttggcttgg atgactttga gtctctgaaa gttataggaa
      361 gaggagcttt tggagaggtg cggttggtcc agaagaaaga tacaggccat atctatgcaa
      421 tgaagatatt gagaaagtct gatatgcttg aaaaagagca ggtggcccat atccgagcag
      481 aaagagatat tttggtagaa gcagatggtg cctgggtggt gaagatgttt tacagttttc
      541 aggataagag gaatctttat ctaatcatgg aatttctccc tggaggtgac atgatgacat
      601 tgctaatgaa gaaagacacc ttgacagaag aggaaacaca gttctacatt tcagagactg
      661 ttctggcaat agatgcgatc caccagttgg gtttcatcca tcgggatatt aagccagaca
      721 accttttatt ggatgccaag ggtcatgtaa aattatctga ttttggttta tgtacgggat
      781 taaagaaagc tcacaggact gaattttata gaaatctcac acacaaccca ccaagtgact
      841 tctcatttca gaacatgaac tcaaagagga aagcagaaac ttggaagaag aacaggagac
      901 aactggcata ttccacagtt gggacaccag attacattgc tccgaagtat tcatgcagac
      961 tggttccaca aattgtgtga ctggtggtc
//
