LOCUS       sequence-174574-        8849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing UAS-Syt::7xHalo7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: UAS-Syt::7xHalo7
FEATURES             Location/Qualifiers
     source          1..8849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..90)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        157..261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(262..330,331..1122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(480..499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1293..1881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1782..1801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1968..2037
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2262..2280)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2732..2803,3205..3478,3553..4207,4269..4584,4788..4919,
                     4990..5604)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6634..6728
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6762..6856
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6887..7125
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7072..7093
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7140..7206
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 caatggagct ggcttggata gcgattcgag ttaacggccg gccgtttaaa cgaattcttg
       61 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      121 ttcttagact caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      241 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      301 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      361 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      421 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      481 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      541 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      601 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      661 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      721 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      781 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      841 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      901 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      961 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1021 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1081 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1141 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1201 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1261 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1321 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1381 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1441 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1501 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1561 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1621 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1681 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1741 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1801 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1861 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1921 tgctggcctt ttgctcacat gttaccgtcg acgatgtagg tcacggtctc gaagccgcgg
     1981 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg
     2041 tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc
     2101 gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg
     2161 acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg
     2221 ggcatgtcga caagccgaac atatgggcgc gcctagtatg tatgtaagtt aataaaaccc
     2281 atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg
     2341 aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct
     2401 ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc
     2461 tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc
     2521 gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt
     2581 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
     2641 ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg
     2701 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
     2761 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc
     2821 atacatctta agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg
     2881 cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg
     2941 aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg
     3001 atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa
     3061 ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc
     3121 gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat
     3181 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac
     3241 cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac
     3301 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac
     3361 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc
     3421 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt
     3481 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa
     3541 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt
     3601 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc
     3661 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc
     3721 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg
     3781 aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag
     3841 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca
     3901 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg
     3961 cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg
     4021 actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga
     4081 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc
     4141 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct
     4201 tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc
     4261 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag
     4321 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc
     4381 gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat
     4441 ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg
     4501 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta
     4561 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct
     4621 tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt
     4681 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat
     4741 ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
     4801 tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg
     4861 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
     4921 taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat
     4981 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
     5041 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca
     5101 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
     5161 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
     5221 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
     5281 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
     5341 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
     5401 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
     5461 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
     5521 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
     5581 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
     5641 tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta
     5701 tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca
     5761 tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct
     5821 tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt atatacatac
     5881 ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct
     5941 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
     6001 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
     6061 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
     6121 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
     6181 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
     6241 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
     6301 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
     6361 cactggaact agggcgcgcc tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     6421 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     6481 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     6541 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     6601 tggccagggc cgcaagcttg catgcctgca ggtcggagta ctgtcctccg agcggagtac
     6661 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     6721 cctccgagcg gagactctag ccctagggca tgcctgcagg tcggagtact gtcctccgag
     6781 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     6841 agtactgtcc tccgagcgga gactctagcg ctagcgacgt cgagcgccgg agtataaata
     6901 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     6961 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     7021 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     7081 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag atctaaaagg
     7141 taggttcaac cactgatgcc taggcacacc gaaacgacta accctaattc ttatccttta
     7201 cttcaggcgg ccgcaactcc taaaaaaccg ccaccatgcc gccaaatgca aaatcggaaa
     7261 cggacgccaa accggaagcg gaaccagcgc cagcgtccga gccggcagcg gaactggagt
     7321 ccgtcgacca gaagctggaa gaaacacatc actccaaatt ccgtgaagtg gacaggcagg
     7381 agcaggaggt gctcgccgag aaggcggcgg aagcggccag tcaaagaatc gcacaggtgg
     7441 aatcaacaac acggagtgct accacagagg ctcaggaatc caccaccacc gcagtgccgg
     7501 tgatcaaaaa gatcgagcat gtcggcgaag tggtcaccga ggtgatcgcg gagcgcacgg
     7561 ggctgcccac atggggcgtg gtggccatca tcatactcgt gttcctcgtc gtctttggta
     7621 taatcttctt ctgtgtgcgg agattcctga agaagcgaag aaccaaagat ggaaagggta
     7681 agaagggtgt cgacatgaag tcggtacagt tgttgggatc ggcgtacaag gagaaagttc
     7741 agcctgatat ggaggaactc accgaaaatg ccgaggaggg tgacgaggag gacaagcaga
     7801 gcgagcagaa gctggggcgc ctcaacttca agctggaata cgatttcaac tccaacagtt
     7861 tggccgtgac ggtgatccaa gccgaggagc ttcccgccct ggatatgggc ggtacctcgg
     7921 atccctatgt caaggtgtac ttgctgcccg acaagaagaa gaagttcgag accaaggtgc
     7981 accgcaagac actgagtccg gtcttcaacg agacgttcac ctttaagggt ttgccctacg
     8041 ccgatgccat gaacaagacg ctcgtgtttg ccattttcga cttcgatcgc ttctcgaagc
     8101 acgaccagat cggcgaggtg aaggtgccgc tgtgcaccat cgatctggcg cagacaatcg
     8161 aggagtggcg cgacctggtc agcgttgagg gagagggcgg acaggaaaag ctgggagata
     8221 tctgcttctc gctgcgctac gtgccgaccg ccggaaagct aaccgtcgtc atcctggagg
     8281 ccaagaactt gaagaagatg gacgtgggcg gactgtctga tccatatgtg aaaattgcaa
     8341 tcatgcaaaa tggcaaacgt ttgaaaaaga ggaagacaag tgtcaaaaaa tgcaccctca
     8401 acccttacta taatgagtcg ttctcatttg aagtaccatt tgaacaaatg caaaaaatct
     8461 gtctcgctgt gaccgtcgtg gactacgatc gtattggcac ctccgaaccc atcggccgct
     8521 gcatacttgg ctgcatgggc accggaaccg agctgcgtca ctggtcggac atgttggcct
     8581 cgccccgccg gcccatcgcc cagtggcaca ccctcaagga tcccgaggag accgacgaga
     8641 tcctgaagaa catgaagacc ggttctagag gatccatggc agaaatcggt actggctttc
     8701 cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc
     8761 cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc
     8821 gcaacatcat cccgcatgtt gcaccgacc
//
