LOCUS       sequence-174577-       14683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Cortez D, Wang Y, Qin J, Elledge SJ
  TITLE     Requirement of ATM-dependent phosphorylation of brca1 in the DNA 
            damage response to double-strand breaks.
  JOURNAL   Science. 1999 Nov 5. 286(5442):1162-6.
  PUBMED    10550055
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1999 Nov 5. 286(5442):1162-6."
COMMENT     Sequence Label: pcDNAFLAG-ATM-kd
FEATURES             Location/Qualifiers
     source          1..14683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     260..279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             414..1208
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(468..487)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1078..1097
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1382..1503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1419..1438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1473..1492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1552..1568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1552..1568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1565..1587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1576..1592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1600..1606,1607..1624,1625..1630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1645..1666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1783..1800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1954..2542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2034..2053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2713..3504,3505..3573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3336..3355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3574..3678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3753..3772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3944..4323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4324..4527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4478..4498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4572..4591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4572..4590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4693..4716
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4720..4737
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(13945..13963)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13946..13963)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13983..14000)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13989..14213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(14259..14683,1..4)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14346..14365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14556..14577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      361 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
      421 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
      481 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
      541 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
      601 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
      661 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
      721 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
      781 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
      841 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
      901 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
      961 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1021 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1081 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1141 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1201 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     1261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     1321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3721 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3781 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3841 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3901 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3961 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4021 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4081 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4141 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4201 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4261 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4321 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4381 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4441 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4501 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     4561 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc
     4621 cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac tggcggccgc
     4681 tcgaccacca tggactacaa ggacgatgac gacaagatgc atcatcatca tcatcatatg
     4741 agtctagtac ttaatgatct gcttatctgc tgccgtcaac tagaacatga tagagctaca
     4801 gaacgaaaga aagaagttga gaaatttaag cgcctgattc gagatcctga aacaattaaa
     4861 catctagatc ggcattcaga ttccaaacaa ggaaaatatt tgaattggga tgctgttttt
     4921 agatttttac agaaatatat tcagaaagaa acagaatgtc tgagaatagc aaaaccaaat
     4981 gtatcagcct caacacaagc ctccaggcag aaaaagatgc aggaaatcag tagtttggtc
     5041 aaatacttca tcaaatgtgc aaacagaaga gcacctaggc taaaatgtca agaactctta
     5101 aattatatca tggatacagt gaaagattca tctaatggtg ctatttacgg agctgattgt
     5161 agcaacatac tactcaaaga cattctttct gtgagaaaat actggtgtga aatatctcag
     5221 caacagtggt tagaattgtt ctctgtgtac ttcaggctct atctgaaacc ttcacaagat
     5281 gttcatagag ttttagtggc tagaataatt catgctgtta ccaaaggatg ctgttctcag
     5341 actgacggat taaattccaa atttttggac tttttttcca aggctattca gtgtgcgaga
     5401 caagaaaaga gctcttcagg tctaaatcat atcttagcag ctcttactat cttcctcaag
     5461 actttggctg tcaactttcg aattcgagtg tgtgaattag gagatgaaat tcttcccact
     5521 ttgctttata tttggactca acataggctt aatgattctt taaaagaagt cattattgaa
     5581 ttatttcaac tgcaaattta tatccatcat ccgaaaggag ccaaaaccca agaaaaaggt
     5641 gcttatgaat caacaaaatg gagaagtatt ttatacaact tatatgatct gctagtgaat
     5701 gagataagtc atataggaag tagaggaaag tattcttcag gatttcgtaa tattgccgtc
     5761 aaagaaaatt tgattgaatt gatggcagat atctgtcacc aggtttttaa tgaagatacc
     5821 agatccttgg agatttctca atcttacact actacacaaa gagaatctag tgattacagt
     5881 gtcccttgca aaaggaagaa aatagaacta ggctgggaag taataaaaga tcaccttcag
     5941 aagtcacaga atgattttga tcttgtgcct tggctacaga ttgcaaccca attaatatca
     6001 aagtatcctg caagtttacc taactgtgag ctgtctccat tactgatgat actatctcag
     6061 cttctacccc aacagcgaca tggggaacgt acaccatatg tgttacgatg ccttacggaa
     6121 gttgcattgt gtcaagacaa gaggtcaaac ctagaaagct cacaaaagtc agatttatta
     6181 aaactctgga ataaaatttg gtgtattacc tttcgtggta taagttctga gcaaatacaa
     6241 gctgaaaact ttggcttact tggagccata attcagggta gtttagttga ggttgacaga
     6301 gaattctgga agttatttac tgggtcagcc tgcagacctt catgtcctgc agtatgctgt
     6361 ttgactttgg cactgaccac cagtatagtt ccaggaacgg taaaaatggg aatagagcaa
     6421 aatatgtgtg aagtaaatag aagcttttct ttaaaggaat caataatgaa atggctctta
     6481 ttctatcagt tagagggtga cttagaaaat agcacagaag tgcctccaat tcttcacagt
     6541 aattttcctc atcttgtact ggagaaaatt cttgtgagtc tcactatgaa aaactgtaaa
     6601 gctgcaatga attttttcca aagcgtgcca gagtgtgaac accaccaaaa agataaagaa
     6661 gaactttcat tctcagaagt agaagaacta tttcttcaga caacttttga caagatggac
     6721 tttttaacca ttgtgagaga atgtggtata gaaaagcacc agtccagtat tggcttctct
     6781 gtccaccaga atctcaagga atcactggat cgctgtcttc tgggattatc agaacagctt
     6841 ctgaataatt actcatctga gattacaaat tcagaaactc ttgtccggtg ttcacgtctt
     6901 ttggtgggtg tccttggctg ctactgttac atgggtgtaa tagctgaaga ggaagcatat
     6961 aagtcagaat tattccagaa agccaagtct ctaatgcaat gtgcaggaga aagtatcact
     7021 ctgtttaaaa ataagacaaa tgaggaattc agaattggtt ccttgagaaa tatgatgcag
     7081 ctatgtacac gttgcttgag caactgtacc aagaagagtc caaataagat tgcatctggc
     7141 tttttcctgc gattgttaac atcaaagcta atgaatgaca ttgcagatat ttgtaaaagt
     7201 ttagcatcct tcatcaaaaa gccatttgac cgtggagaag tagaatcaat ggaagatgat
     7261 actaatggaa atctaatgga ggtggaggat cagtcatcca tgaatctatt taacgattac
     7321 cctgatagta gtgttagtga tgcaaacgaa cctggagaga gccaaagtac cataggtgcc
     7381 attaatcctt tagctgaaga atatctgtca aagcaagatc tacttttctt agacatgctc
     7441 aagttcttgt gtttgtgtgt aactactgct cagaccaata ctgtgtcctt tagggcagct
     7501 gatattcgga ggaaattgtt aatgttaatt gattctagca cgctagaacc taccaaatcc
     7561 ctccacctgc atatgtatct aatgctttta aaggagcttc ctggagaaga gtaccccttg
     7621 ccaatggaag atgttcttga acttctgaaa ccactatcca atgtgtgttc tttgtatcgt
     7681 cgtgaccaag atgtttgtaa aactatttta aaccatgtcc ttcatgtagt gaaaaaccta
     7741 ggtcaaagca atatggactc tgagaacaca agggatgctc aaggacagtt tctaacagta
     7801 attggagcat tttggcatct aacaaaggag aggaaatata tattctctgt aagaatggcc
     7861 ctagtaaatt gccttaaaac tttgcttgag gctgatcctt attcaaaatg ggccattctt
     7921 aatgtaatgg gaaaagactt tcctgtaaat gaagtattta cacaatttct tgctgacaat
     7981 catcaccaag ttcgcatgtt ggctgcagag tcaatcaata gattgttcca ggacacgaag
     8041 ggagattctt ccaggttact gaaagcactt cctttgaagc ttcagcaaac agcttttgaa
     8101 aatgcatact tgaaagctca ggaaggaatg agagaaatgt cccatagtgc tgagaaccct
     8161 gaaactttgg atgaaattta taatagaaaa tctgttttac tgacgttgat agctgtggtt
     8221 ttatcctgta gccctatctg cgaaaaacag gctttgtttg ccctgtgtaa atctgtgaaa
     8281 gagaatggat tagaacctca ccttgtgaaa aaggttttag agaaagtttc tgaaactttt
     8341 ggatatagac gtttagaaga ctttatggca tctcatttag attatctggt tttggaatgg
     8401 ctaaatcttc aagatactga atacaactta tcttcttttc cttttatttt attaaactac
     8461 acaaatattg aggatttcta tagatcttgt tataaggttt tgattccaca tctggtgatt
     8521 agaagtcatt ttgatgaggt gaagtccatt gctaatcaga ttcaagagga ctggaaaagt
     8581 cttctaacag actgctttcc aaagattctt gtaaatattc ttccttattt tgcctatgag
     8641 ggtaccagag acagtgggat ggcacagcaa agagagactg ctaccaaggt ctatgatatg
     8701 cttaaaagtg aaaacttatt gggaaaacag attgatcact tattcattag taatttacca
     8761 gagattgtgg tggagttatt gatgacgtta catgagccag caaattctag tgccagtcag
     8821 agcactgacc tctgtgactt ttcaggggat ttggatcctg ctcctaatcc acctcatttt
     8881 ccatcgcatg tgattaaagc aacatttgcc tatatcagca attgtcataa aaccaagtta
     8941 aaaagcattt tagaaattct ttccaaaagc cctgattcct atcagaaaat tcttcttgcc
     9001 atatgtgagc aagcagctga aacaaataat gtttataaga agcacagaat tcttaaaata
     9061 tatcacctgt ttgttagttt attactgaaa gatataaaaa gtggcttagg aggagcttgg
     9121 gcctttgttc ttcgagacgt tatttatact ttgattcact atatcaacca aaggccttct
     9181 tgtatcatgg atgtgtcatt acgtagcttc tccctttgtt gtgacttatt aagtcaggtt
     9241 tgccagacag ccgtgactta ctgtaaggat gctctagaaa accatcttca tgttattgtt
     9301 ggtacactta taccccttgt gtatgagcag gtggaggttc agaaacaggt attggacttg
     9361 ttgaaatact tagtgataga taacaaggat aatgaaaacc tctatatcac gattaagctt
     9421 ttagatcctt ttcctgacca tgttgttttt aaggatttgc gtattactca gcaaaaaatc
     9481 aaatacagta gaggaccctt ttcactcttg gaggaaatta accattttct ctcagtaagt
     9541 gtttatgatg cacttccatt gacaagactt gaaggactaa aggatcttcg aagacaactg
     9601 gaactacata aagatcagat ggtggacatt atgagagctt ctcaggataa tccgcaagat
     9661 gggattatgg tgaaactagt tgtcaatttg ttgcagttat ccaagatggc aataaaccac
     9721 actggtgaaa aagaagttct agaggctgtt ggaagctgct tgggagaagt gggtcctata
     9781 gatttctcta ccatagctat acaacatagt aaagatgcat cttataccaa ggcccttaag
     9841 ttatttgaag ataaagaact tcagtggacc ttcataatgc tgacctacct gaataacaca
     9901 ctggtagaag attgtgtcaa agttcgatca gcagctgtta cctgtttgaa aaacatttta
     9961 gccacaaaga ctggacatag tttctgggag atttataaga tgacaacaga tccaatgctg
    10021 gcctatctac agccttttag aacatcaaga aaaaagtttt tagaagtacc cagatttgac
    10081 aaagaaaacc cttttgaagg cctggatgat ataaatctgt ggattcctct aagtgaaaat
    10141 catgacattt ggataaagac actgacttgt gcttttttgg acagtggagg cacaaaatgt
    10201 gaaattcttc aattattaaa gccaatgtgt gaagtgaaaa ctgacttttg tcagactgta
    10261 cttccatact tgattcatga tattttactc caagatacaa atgaatcatg gagaaatctg
    10321 ctttctacac atgttcaggg atttttcacc agctgtcttc gacacttctc gcaaacgagc
    10381 cgatccacaa cccctgcaaa cttggattca gagtcagagc actttttccg atgctgtttg
    10441 gataaaaaat cacaaagaac aatgcttgct gttgtggact acatgagaag acaaaagaga
    10501 ccttcttcag gaacaatttt taatgatgct ttctggctgg atttaaatta tctagaagtt
    10561 gccaaggtag ctcagtcttg tgctgctcac tttacagctt tactctatgc agaaatctat
    10621 gcagataaga aaagtatgga tgatcaagag aaaagaagtc ttgcatttga agaaggaagc
    10681 cagagtacaa ctatttctag cttgagtgaa aaaagtaaag aagaaactgg aataagttta
    10741 caggatcttc tcttagaaat ctacagaagt ataggggagc cagatagttt gtatggctgt
    10801 ggtggaggga agatgttaca acccattact agactacgaa catatgaaca cgaagcaatg
    10861 tggggcaaag ccctagtaac atatgacctc gaaacagcaa tcccctcatc aacacgccag
    10921 gcaggaatca ttcaggcctt gcagaatttg ggactctgcc atattctttc cgtctattta
    10981 aaaggattgg attatgaaaa taaagactgg tgtcctgaac tagaagaact tcattaccaa
    11041 gcagcatgga ggaatatgca gtgggaccat tgcacttccg tcagcaaaga agtagaagga
    11101 accagttacc atgaatcatt gtacaatgct ctacaatctc taagagacag agaattctct
    11161 acattttatg aaagtctcaa atatgccaga gtaaaagaag tggaagagat gtgtaagcgc
    11221 agccttgagt ctgtgtattc gctctatccc acacttagca ggttgcaggc cattggagag
    11281 ctggaaagca ttggggagct tttctcaaga tcagtcacac atagacaact ctctgaagta
    11341 tatattaagt ggcagaaaca ctcccagctt ctcaaggaca gtgattttag ttttcaggag
    11401 cctatcatgg ctctacgcac agtcattttg gagatcctga tggaaaagga aatggacaac
    11461 tcacaaagag aatgtattaa ggacattctc accaaacacc ttgtagaact ctctatactg
    11521 gccagaactt tcaagaacac tcagctccct gaaagggcaa tatttcaaat taaacagtac
    11581 aattcagtta gctgtggagt ctctgagtgg cagctggaag aagcacaagt attctgggca
    11641 aaaaaggagc agagtcttgc cctgagtatt ctcaagcaaa tgatcaagaa gttggatgcc
    11701 agctgtgcag cgaacaatcc cagcctaaaa cttacataca cagaatgtct gagggtttgt
    11761 ggcaactggt tagcagaaac gtgcttagaa aatcctgcgg tcatcatgca gacctatcta
    11821 gaaaaggcag tagaagttgc tggaaattat gatggagaaa gtagtgatga gctaagaaat
    11881 ggaaaaatga aggcatttct ctcattagcc cggttttcag atactcaata ccaaagaatt
    11941 gaaaactaca tgaaatcatc ggaatttgaa aacaagcaag ctctcctgaa aagagccaaa
    12001 gaggaagtag gtctccttag ggaacataaa attcagacaa acagatacac agtaaaggtt
    12061 cagcgagagc tggagttgga tgaattagcc ctgcgtgcac tgaaagagga tcgtaaacgc
    12121 ttcttatgta aagcagttga aaattatatc aactgcttat taagtggaga agaacatgat
    12181 atgtgggtat tccgactttg ttccctctgg cttgaaaatt ctggagtttc tgaagtcaat
    12241 ggcatgatga agagagacgg aatgaagatt ccaacatata aatttttgcc tcttatgtac
    12301 caattggctg ctagaatggg gaccaagatg atgggaggcc taggatttca tgaagtcctc
    12361 aataatctaa tctctagaat ttcaatggat cacccccatc acactttgtt tattatactg
    12421 gccttagcaa atgcaaacag agatgaattt ctgactaaac cagaggtagc cagaagaagc
    12481 agaataacta aaaatgtgcc taaacaaagc tctcagcttg atgaggatcg aacagaggct
    12541 gcaaatagaa taatatgtac tatcagaagt aggagacctc agatggtcag aagtgttgag
    12601 gcactttgtg atgcttatat tatattagca aacttagatg ccactcagtg gaagactcag
    12661 agaaaaggca taaatattcc agcagaccag ccaattacta aacttaagaa tttagaagat
    12721 gttgttgtcc ctactatgga aattaaggtg gaccacacag gagaatatgg aaatctggtg
    12781 actatacagt catttaaagc agaatttcgc ttagcaggag gtgtaaattt accaaaaata
    12841 atagattgtg taggttccga tggcaaggag aggagacagc ttgttaaggg ccgtgatgac
    12901 ctgagacaag atgctgtcat gcaacaggtc ttccagatgt gtaatacatt actgcagaga
    12961 aacacggaaa ctaggaagag gaaattaact atctgtactt ataaggtggt tcccctctct
    13021 cagcgaagtg gtgttcttga atggtgcaca ggaactgtcc ccattggtga atttcttgtt
    13081 aacaatgaag atggtgctca taaaagatac aggccaaatg atttcagtgc ctttcagtgc
    13141 caaaagaaaa tgatggaggt gcaaaaaaag tcttttgaag agaaatatga agtcttcatg
    13201 gatgtttgcc aaaattttca accagttttc cgttacttct gcatggaaaa attcttggat
    13261 ccagctattt ggtttgagaa gcgattggct tatacgcgca gtgtagctac ttcttctatt
    13321 gttggttaca tacttggact tggtgctaga catgtacaga agatcttgat aaatgagcag
    13381 tcagcagaac ttgtacatat agatctaggt gttgcttttg aacagggcaa aatccttcct
    13441 actcctgaga cagttccttt tagactcacc agagatattg tggatggcat gggcattacg
    13501 ggtgttgaag gtgtcttcag aagatgctgt gagaaaacca tggaagtgat gagaaactct
    13561 caggaaactc tgttaaccat tgtagaggtc cttctatatg atccactctt tgactggacc
    13621 atgaatcctt tgaaagcttt gtatttacag cagaggccgg aagatgaaac tgagcttcac
    13681 cctactctga atgcagatga ccaagaatgc aaacgaaatc tcagtgatat tgaccagagt
    13741 ttcaacaaag tagctgaacg tgtcttaatg agactacaag agaaactgaa aggagtggaa
    13801 gaaggcactg tgctcagtgt tggtggacaa gtgaatttgc tcatacagca ggccatagac
    13861 cccaaaaatc tcagccgact tttcccagga tggaaagctt gggtgtgatc ttcagtactc
    13921 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
    13981 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    14041 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    14101 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    14161 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    14221 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
    14281 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    14341 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    14401 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    14461 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    14521 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    14581 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    14641 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcg
//
