LOCUS       sequence-174588-        6191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR
FEATURES             Location/Qualifiers
     source          1..6191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(510..1064,1065..1095)
                     /label=P element 5' end
     rep_origin      complement(1330..1918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1410..1429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2089..2880,2881..2949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2712..2731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2950..3054)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3122..3140
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3178..3200)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3300..3319
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(3857..3887,3888..4089)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        4126..4578
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4525..4546
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
       61 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
      121 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
      181 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
      241 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
      301 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
      361 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
      421 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
      481 tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc
      541 caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac
      601 gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact tactctgttt
      661 gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac
      721 taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag
      781 ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc
      841 acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt
      901 ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt
      961 aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa
     1021 acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta
     1081 tgttatttca tcatgggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     1141 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     1201 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     1261 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     1321 gaaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3061 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3121 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3181 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     3241 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3301 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcaccgaat
     3361 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga
     3421 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat
     3481 gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta
     3541 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca
     3601 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata
     3661 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta
     3721 aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc
     3781 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg
     3841 atgagaatgg ccagaccatg atgaaataac ataaggtggt cccgtcggca agagacatcc
     3901 acttaacgta tgcttgcaat aagtgcgagt gaaaggaata gtattctgag tgtcgtattg
     3961 agtctgagtg agacagcgat atgattgttg attaaccctt agcatgtccg tggggtttga
     4021 attaactcat aatattaatt agacgaaatt atttttaaag ttttattttt aataatttgc
     4081 gagtacgcaa agcttggctg catccaacgc gttgggagct ctccgctaga atcccaaaac
     4141 aaactggtta ttgtggtagg tcatttgttt ggcagaaaga aaactcgaga aatttctctg
     4201 gccgttattc gttattctct cttttctttt tgggtctctc cctctctgca ctaatgctct
     4261 ctcactctgt cacacagtaa acggcatact gctctcgttg gttcgagaga gcgcgcctcg
     4321 aatgttcgcg aaaagagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat
     4381 tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg
     4441 cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt
     4501 aaccagcaac caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa
     4561 tacaagaaga gaactctgaa tacaaaatgc gagccctggt cgtcatccgt ttgtcccgtg
     4621 tcaccgatgc tacgacgtcc ccggagcgtc agctggagtc gtgccagcag ctctgcgccc
     4681 agcgcggctg ggatgtcgtc ggagtggctg aggatctgga tgtctccgga gctgtcgatc
     4741 cgttcgatcg taagcgccgt ccaaacctgg cccgttggct ggctttcgag gagcaaccgt
     4801 tcgatgtgat cgtggcttac cgtgtcgacc gtttgacccg atcgattcgt catctgcagc
     4861 agctggtcca ctgggctgag gatcacaaga agttggtcgt ctccgctacc gaagctcact
     4921 tcgatacgac cacgccattc gctgctgtcg tgatcgctct catgggaacg gtggctcaga
     4981 tggaattgga ggctatcaaa gagcgtaacc gtagcgctgc ccatttcaac attcgtgccg
     5041 gaaagtaccg tggaagcttg ccaccgtggg gttacttgcc aacgcgtgtg gatggagagt
     5101 ggcgtctgtt ggtcgatccg gtgcagcgtg agcgtattct ggaggtctac caccgtgtcg
     5161 tggataacca cgagccgttg cacttggtgg ctcacgattt gaaccagcgt ggaattctga
     5221 gcccgaagga ttacttcgct cagttgcagg gacgtgagcc gcagggacgt gcttggtcgg
     5281 ctaccgcctt gaagcgtagc ttgatctccg aggctatgtt gggatacgct accttgaacg
     5341 gaaagaccgt gcgtgatgat gatggagctc cgttggtgcg tgctgagccg atcttgacgc
     5401 gtgagcagtt ggaggctctg cgcgccgagt tggtgaagac cgatcgtacc aagccggctg
     5461 tgagcacccc gagcctgttg ctgcgtgtgt tgttctgcgc tgtgtgcgga gagcccgctt
     5521 acaagttcgc tggaggaggc cgtaagcacc cgcgttaccg ttgccgttcc atgggattcc
     5581 cgaagcactg cggaaacgga accgtcgcta tggccgagtg ggatgctttc tgcgaggagc
     5641 aggtgctgga tctgttggga gacgctgagc gtctggagaa ggtctgggtc gctggctccg
     5701 atgctgccgt ggagttggct gagttgaacg ctgagttggt ggatttgacg agcttgattg
     5761 gaagcccggc ttaccgcgcc ggcagcccgc agcgtgaggc tctggatgct cgtatcgagg
     5821 ctctggccgc tcgtcaggag gagttggagg gactggaggc tcgcccgtcc ggatgggagt
     5881 ggcgtgagac cggacagcgt ttcggagatt ggtggcgtga gcaggatacc gctgctaaga
     5941 acacctggtt gcgtagcatg aacgtgcgtc tgaccttcga tgtccgtgga ggattgaccc
     6001 gtaccatcga tttcggagat ctgcaggagt acgagcagca cttgcgtctg ggcagcgtgg
     6061 tcgagcgttt gcacaccgga atgagctaag actaaagcca aatagaaaat tattcagttc
     6121 ctggcttaag tttttaaaag tgatattatt tatttggttg taaccaacca aaagaatgta
     6181 aataactaat a
//
