LOCUS       sequence-174590-       12496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag tagged nucleoporin Tpr resistant to siRNA 
            GCACAACAGGATAAGGTTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12496)
  AUTHORS   Rajanala K, Nandicoori VK
  TITLE     Localization of nucleoporin Tpr to the nuclear pore complex is 
            essential for Tpr mediated regulation of the export of unspliced 
            RNA.
  JOURNAL   PLoS One. 2012;7(1):e29921. doi: 10.1371/journal.pone.0029921. Epub 
            2012 Jan 13.
  PUBMED    22253824
REFERENCE   2  (bases 1 to 12496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1):e29921. doi"; pages: " 
            10.1371/journal.pone.0029921. Epub 2012 Jan 13"
COMMENT     Sequence Label: pcDNA3-Flag-Tpr-Si
FEATURES             Location/Qualifiers
     source          1..12496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     652..672
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     746..765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        746..764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             837..860
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(7932..7950)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7933..7950)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7970..7987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7976..8200
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8246..8674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8333..8352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8543..8564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8683..8703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8688..9017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8868..9003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8930..8949
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9084..9878
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9138..9157)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9748..9767
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10052..10173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10089..10108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10143..10162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10222..10238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10222..10238)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10235..10257)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10246..10262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10270..10276,10277..10294,10295..10300))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10315..10336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10453..10470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10624..11212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10704..10723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11383..12174,12175..12243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12006..12025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12244..12348)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12423..12442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
       61 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      121 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      181 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      241 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      301 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      361 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      421 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      481 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      541 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      601 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      661 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
      721 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
      781 accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgcc accatggact
      841 acaaggacga cgatgacaag ccagcggccg ctatggcggc ggtgttgcag caagtcctgg
      901 agcgcacgga gctgaacaag ctgcccaagt ctgtccagaa caaacttgaa aagttccttg
      961 ctgatcagca atccgagatc gatggcctga aggggcggca tgagaaattt aaggtggaga
     1021 gcgaacaaca gtattttgaa atagaaaaga ggttgtccca cagtcaggag agacttgtga
     1081 atgaaacccg agagtgtcaa agcttgcggc ttgagctaga gaaactcaac aatcaactga
     1141 aggcactaac tgagaaaaac aaagaacttg aaattgctca ggatcgcaat attgccattc
     1201 agagccaatt tacaagaaca aaggaagaat tagaagctga gaaaagagac ttaattagaa
     1261 ccaatgagag actatctcaa gaacttgaat acttaacaga ggatgttaaa cgtctgaatg
     1321 aaaaacttaa agaaagcaat acaacaaagg gtgaacttca gttaaaattg gatgaacttc
     1381 aagcttctga tgtttctgtt aagtatcgag aaaaacgctt ggagcaagaa aaggaattgc
     1441 tacatagtca gaatacatgg ctgaatacag agttgaaaac caaaactgat gaacttctgg
     1501 ctcttggaag agaaaaaggg aatgagattc tagagcttaa atgtaatctt gaaaataaaa
     1561 aagaagaggt ttctagactg gaagaacaaa tgaatggctt aaaaacatca aatgaacatc
     1621 ttcaaaagca tgtggaggat ctgttgacca aattaaaaga ggccaaggaa caacaggcca
     1681 gtatggaaga gaaattccac aatgaattaa atgcccacat aaaactttct aatttgtaca
     1741 agagtgccgc tgatgactca gaagcaaaga gcaatgaact aacccgggca gtagaggaac
     1801 tacacaaact tttgaaagaa gctggtgaag ccaacaaagc aatacaagat catcttctag
     1861 aggtggagca atccaaagat caaatggaaa aagaaatgct tgagaaaata gggagattgg
     1921 agaaggaatt agagaatgca aatgaccttc tttctgccac aaaacgtaaa ggagccatat
     1981 tgtctgaaga agagcttgcc gccatgtctc ctactgcagc agctgtagct aagatagtga
     2041 aacctgggat gaaactaact gagctctata atgcttatgt ggaaactcag gatcagttgc
     2101 ttttggagaa actagagaac aaaagaatta ataagtacct agatgaaata gtgaaagaag
     2161 tggaagccaa agcaccaatt ttgaaacgcc agcgtgagga atatgaacgt gcacagaaag
     2221 ctgtagcaag tttatctgtt aagcttgaac aagctatgaa ggagattcag cgattgcagg
     2281 aggacactga taaagccaac aagcaatcat ctgtacttga gagagataat cgaagaatgg
     2341 aaatacaagt aaaagatctt tcacaacaga ttagagtgct tttgatggaa cttgaagaag
     2401 caaggggtaa ccacgtaatt cgtgatgagg aagtaagctc tgctgatata agtagttcat
     2461 ctgaggtaat atcacagcat ctagtatctt acagaaatat tgaagagctt caacaacaaa
     2521 atcaacgtct cttagtggcc cttagagagc ttggggaaac cagagaaaga gaagaacaag
     2581 aaacaacttc atccaaaatc actgagcttc agctcaaact tgagagtgcc cttactgaac
     2641 tagaacaact ccgcaaatca cgacagcatc aaatgcagct tgttgattcc atagttcgtc
     2701 agcgtgatat gtaccgtatt ttattgtcac aaacaacagg agttgccatt ccattacatg
     2761 cttcaagctt agatgatgtt tctcttgcat caactccaaa acgtccaagt acatcacaga
     2821 ctgtttccac tcctgctcca gtacctgtta ttgaatcaac agaggctata gaggctaagg
     2881 ctgcccttaa acagttgcag gaaatttttg agaactacaa aaaagaaaaa gcagaaaatg
     2941 aaaaaataca aaatgagcag cttgagaaac ttcaagaaca agttacagat ttgcgatcac
     3001 aaaataccaa aatttctacc cagctagatt ttgcttctaa acgttatgaa atgctgcaag
     3061 ataatgttga aggatatcgt cgagaaataa catcacttca tgagagaaat cagaaactca
     3121 ctgccacaac tcaaaagcaa gaacagatta tcaatacgat gactcaagat ttgagaggag
     3181 caaatgagaa gctagctgtc gcagaagtaa gagcagaaaa tttgaagaag gaaaaggaaa
     3241 tgcttaaatt gtctgaagtt cgtctttctc agcaaagaga gtctttgtta gctgaacaaa
     3301 gggggcaaaa cttactgcta actaatctgc aaacaattca gggaatactg gagcgatctg
     3361 aaacagaaac caaacaaagg cttagtagcc agatagaaaa actggaacat gagatctctc
     3421 atctaaagaa gaagttggaa aatgaggtgg aacaaaggca tacacttact agaaatctag
     3481 atgttcaact tttagataca aagagacaac tggatacaga gacaaatctt catcttaaca
     3541 caaaagaact attaaaaaat gctcaaaaag aaattgccac attgaaacag cacctcagta
     3601 atatggaagt ccaagttgct tctcagtctt cacagagaac tggtaaaggt cagcctagca
     3661 acaaagaaga tgtggatgat cttgtgagtc agctaagaca gacagaagag caggtgaatg
     3721 acttaaagga gagactcaaa acaagtacga gcaatgtgga acaatatcaa gcaatggtta
     3781 ctagtttaga agaatccctg aacaaggaaa aacaggtgac agaagaagtg cgtaagaata
     3841 ttgaagttcg tttaaaagag tcagctgaat ttcagacaca gttggaaaag aagttgatgg
     3901 aagtagagaa ggaaaaacaa gaacttcagg atgataaaag aagagccata gagagcatgg
     3961 aacaacagtt atctgaattg aagaaaacac tttctagtgt tcagaatgaa gtacaagaag
     4021 ctcttcagag agcaagcaca gctttaagta atgagcagca agccagacgt gactgtcagg
     4081 aacaagctaa aatagctgtg gaagctcaga ataagtatga gagagaattg atgctgcatg
     4141 ctgctgatgt tgaagctcta caagctgcga aggagcaggt ttcaaaaatg gcatcagtcc
     4201 gtcagcattt ggaagaaaca acacagaaag cagaatcaca gttgttggag tgtaaagcat
     4261 cttgggagga aagagagaga atgttaaagg atgaagtttc caaatgtgta tgtcgctgtg
     4321 aagatctgga gaaacaaaac agattacttc atgatcagat cgaaaaatta agtgacaagg
     4381 tcgttgcctc tgtgaaggaa ggtgtacaag gtccactgaa tgtatctctc agtgaagaag
     4441 gaaaatctca agaacaaatt ttggaaattc tcagatttat acgacgagaa aaagaaattg
     4501 ctgaaactag gtttgaggtg gctcaggttg agagtctgcg ttatcgacaa agggttgaac
     4561 ttttagaaag agagctgcag gaactgcaag atagtctaaa tgctgaaagg gagaaagtcc
     4621 aggtaactgc aaaaacaatg gctcagcatg aagaactgat gaagaaaact gaaacaatga
     4681 atgtagttat ggagaccaat aaaatgctaa gagaagagaa ggagagacta gaacaggatc
     4741 tacagcaaat gcaagcaaag gtgaggaaac tggagttaga tattttaccc ttacaagaag
     4801 caaatgctga gctgagtgag aaaagcggta tgttgcaggc agagaagaag ctcttagaag
     4861 aggatgtcaa acgttggaaa gcacgtaacc agcatctagt aagtcaacag aaagatccag
     4921 atacagaaga atatcggaag ctcctttctg aaaaggaagt tcatactaag cgtattcaac
     4981 aattgacaga agaaattggt agacttaaag ctgaaattgc aagatcaaat gcatctttga
     5041 ctaacaacca gaacttaatt cagagtctga aggaagatct aaataaagta agaactgaaa
     5101 aggaaaccat ccagaaggac ttagatgcca aaataattga tatccaagaa aaagtcaaaa
     5161 ctattactca agttaagaaa attggacgta ggtacaagac tcaatatgaa gaacttaaag
     5221 ctcagcaaga caaagttatg gagacatcgg ctcagtcctc tggagaccat caggagcagc
     5281 atgtttcagt ccaggaaatg caggaactca aagaaacgct caaccaagct gaaacaaaat
     5341 caaaatcact tgaaagtcaa gtagagaatc tgcagaagac attatctgaa aaagagacag
     5401 aagcaagaaa tctccaggaa cagactgtgc aacttcagtc tgaactttca cgacttcgtc
     5461 aggatcttca agatagaacc acacaggagg agcagctccg acaacagata actgaaaagg
     5521 aagaaaaaac cagaaaggct attgtagcag caaagtcaaa aattgcacac ttagctggtg
     5581 taaaagatca gctaactaaa gaaaatgagg agcttaaaca aaggaatgga gccttagatc
     5641 agcagaaaga tgaattggat gttcgcatta ctgcgctaaa gtcccaatat gaaggtcgaa
     5701 ttagtcgctt ggaaagagaa ctcagggagc atcaagagag acaccttgag cagagagatg
     5761 agcctcaaga accttctaat aaggtccctg aacagcagag acagatcaca ttgaaaacaa
     5821 ctccagcttc tggtgaaaga ggaattgcca gcacatcaga cccaccaaca gccaatatca
     5881 agccaactcc tgttgtgtct actccaagta aagtgacagc tgcagctatg gctggaaata
     5941 agtcaacacc cagggctagt atccgcccaa tggttacacc tgcaactgtt acaaatccca
     6001 ctactacccc aacagctaca gtgatgccca ctacacaagt ggaatcacag gaagctatgc
     6061 agtcagaagg gcctgtggaa catgttccag tttttggaag cacaagtgga tccgttcgtt
     6121 ctactagtcc taatgtccag ccttctatct ctcaacctat tttaactgtt cagcaacaaa
     6181 cacaggctac agcttttgtg caacccactc aacagagtca tcctcagatt gagcctgcca
     6241 atcaagagtt atcttcaaac atagtagagg ttgttcagag ttcaccagtt gagcggcctt
     6301 ctacttccac agcagtattt ggcacagttt cggctacccc cagttcttct ttgccaaagc
     6361 gtacacgtga agaggaagag gatagcacca tagaagcatc agaccaagtc tctgatgata
     6421 cagtggaaat gcctcttcca aagaagttga aaagtgtcac acctgtagga actgaggaag
     6481 aagttatggc agaagaaagt actgatggag aggtagagac tcaggtatac aaccaggatt
     6541 ctcaagattc cattggagaa ggagttaccc agggagatta tacacctatg gaagacagtg
     6601 aagaaacctc tcagtctcta caaatagatc ttgggccact tcaatcagat cagcagacga
     6661 caacttcatc ccaggatggt caaggcaaag gagatgatgt cattgtaatt gacagtgatg
     6721 atgaagaaga ggatgaggaa gatgatgatg atgatgaaga tgacacaggg atgggagatg
     6781 agggtgaaga tagtaatgaa ggaactggta gtgccgatgg caatgatggt tatgaagctg
     6841 atgatgctga gggtggtgat gggactgatc caggtacaga aacagaagaa agtatgggtg
     6901 gaggtgaagg taatcacaga gctgctgatt ctcaaaacag tggtgaagga aatacaggtg
     6961 ctgcagaatc ttctttttct caggaggttt ctagagaaca acagccatca tcagcatctg
     7021 aaagacaggc ccctcgagca cctcagtcac cgagacgccc accacatcca cttcccccaa
     7081 gactgaccat tcatgcccca cctcaggagt tgggaccacc agttcagaga attcagatga
     7141 cccgaaggca gtctgtagga cgtggccttc agttgactcc aggaataggt ggcatgcaac
     7201 agcatttttt tgatgatgaa gacagaacag ttccaagtac tccaactctt gtggtgccac
     7261 atcgtactga tggatttgct gaagcaattc attcgccgca ggttgctggt gtccctagat
     7321 tccggtttgg gccacctgaa gatatgccac aaacaagttc tagtcactct gatcttggcc
     7381 agcttgcttc tcaaggaggt ttaggaatgt atgaaacacc cctgttccta gctcatgaag
     7441 aagagtcagg tggccgaagt gttcccacta ctccactaca agtagcagcc ccagtgactg
     7501 tatttactga gagcaccacc tctgatgctt cggaacatgc ctctcaatct gttccaatgg
     7561 tgactacatc cactggcact ttatctacaa caaatgaaac agcaacaggt gatgatggag
     7621 atgaagtatt tgtggaggca gaatctgaag gtattagttc agaagcaggc ctagaaattg
     7681 atagccagca ggaagaagag ccggttcaag catctgatga gtcagatctc ccctccacca
     7741 gccaggatcc tccttctagc tcatctgtag atactagtag tagtcaacca aagcctttca
     7801 gacgagtaag acttcagaca acattgagac aaggtgtccg tggtcgtcag tttaacagac
     7861 agagaggtgt gagccatgca atgggaggga gaggaggaat aaacagagga aatattaatt
     7921 aagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     7981 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8041 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8101 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8161 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8221 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8281 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8341 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8401 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8461 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8521 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8581 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8641 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8761 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8821 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8881 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8941 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9001 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     9061 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     9121 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     9181 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     9241 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     9301 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     9361 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     9421 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     9481 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     9541 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     9601 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     9661 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     9721 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     9781 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     9841 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     9901 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     9961 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    10021 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    10081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    10201 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    10261 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    10321 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    10381 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    10441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11041 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    11101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    11341 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11401 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11461 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11521 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11581 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11641 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11701 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11761 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11821 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11881 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11941 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12001 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12061 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12361 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    12421 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    12481 gaggtcgctg agtagt
//
