LOCUS       sequence-174592-        4562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 
            5'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME
  TITLE     SUV4-20 activity in the preimplantation mouse embryo controls timely
            replication.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5.
  PUBMED    27920088
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2016-11-15"; volume: "30(22)"; pages: "2513-2526. Epub
            2016 Dec 5"
COMMENT     Sequence Label: pRN3P-HA-Suv4-20h2mut
FEATURES             Location/Qualifiers
     source          1..4562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..57)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(39..57)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(64..81)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(64..80)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(73..95)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(222..677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(359..380)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     571..590
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        704..808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(809..877,878..1669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1027..1046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1840..2428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2329..2348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2582..2599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2716..2737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2752..2757,2758..2775,2776..2782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2790..2806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2795..2817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2814..2830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2814..2830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2851..2869
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           2894..2936
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      2944..2953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2956..2982
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2984..3000)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2984..3000)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     3'UTR           4433..4558
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 cccccctgca gggccaagtc ggccggtacc caattcgccc tatagtgagt cgtattacaa
       61 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat
      241 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      301 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
      361 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
      421 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
      481 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      541 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      601 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      661 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2521 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2581 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2641 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2701 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2761 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2821 cagctatgac catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct
     2881 ggagctaagc ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga
     2941 tctgccgcca tgggctaccc atacgatgtt cctgactatg cgggatccac tagttctaga
     3001 atggggcctg atcgagtgac agcccgagaa ctgtgcgaga atgatgacct ggccaccagc
     3061 cttgttcttg acccctacct cggcttccgc acccataaga tgaatgtcag tcctgtgccc
     3121 accttgcgac gacaacacca ccttcgttca gcactggaag ctttcctaag gcaacgggac
     3181 ctggaggctg cattccgggc cctgacgcta ggaggctgga tggcccacta cttccagagc
     3241 cgtgcccctc ggcaggaggc tgctctcaag acccacattt tctgctacct ccgcgccttc
     3301 ctgcctgaga gtggcttcac cattctgccc tgcacccgct actccatgga gaccaatggg
     3361 gccaagattg tgtctacccg tgcttggaag aagaatgaga agctggagct gctggtgggc
     3421 tgcattgcag agctgcgtga agaggatgaa gacctgctta gggccggtga gaacgacttc
     3481 agcatcatgt actccacccg aaaaaggagt gcccagctgt ggcttggccc agctgccttc
     3541 atcgccgctg ccggcaaacc caactgcaag tttgtgccct cagatgggaa tactgcctgt
     3601 gtgaaggtgc tacgggacat tgagccaggg gatgaagtga cttgcttcta tggtgagggc
     3661 ttcttcggcg agaagaatga gcactgtgaa tgctatacct gtgagaggaa aggcgaggga
     3721 gctttcagac ttcagcccag ggagcctgag ctgcgaccaa agcccctaga caagtatgag
     3781 ctccgagaga caaagaggcg tctgcagcag ggcttggtca gcagccagca gagcctgatg
     3841 agccggtggg cctgctccca cctgtcccca ctgcgccctg acccattctg tgctgcctgc
     3901 cagccctcat gcctgctgcc tgccagcccc cacatggatt acttgcccct ttggctccag
     3961 cgggcgcctc aaccccagcc catagtacct cccaggaagc gccaccgccg ccgccgcccc
     4021 aggatccgcc aggcttcatt gcctcctgtc ctccgcactg cctgtgtccc cctgcaccga
     4081 tggggaggct gtggtcccca ctgccagcta cgtgctgagg ccatggtgac cctacacctg
     4141 cgcccccaga ctcgctggac cccacaacag gactggtact gggcccggcg ctatgggcta
     4201 ccttctgtgg ggcgtgtgga acttacccgc ctagccccag ccctgccagc tgctcctgcc
     4261 cctgctggga acccaggtcc tgtccccact cctgatttca tccctaagca ggcccttgcc
     4321 tttgctccgt tctgcccacc taagcgcctc cggctagtgg tcagtcatgg ttctattgat
     4381 ctggacatca atagtggtga gccataagcg gccgcggatc tggttaccac taaaccagcc
     4441 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     4501 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt ttcttcacat
     4561 tc
//
