LOCUS       sequence-174600-        2737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For library amplification of fragment NG-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Gogolok S, Garcia-Diaz C, Pollard SM
  TITLE     STAR: a simple TAL effector assembly reaction using isothermal 
            assembly.
  JOURNAL   Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209.
  PUBMED    27615025
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-12"; volume: "6:33209. doi"; pages: " 
            10.1038/srep33209"
COMMENT     Sequence Label: NG-4
FEATURES             Location/Qualifiers
     source          1..2737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(990..1578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1070..1089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1749..2540,2541..2609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2372..2391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2610..2714)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccatgag ctcgtatcct gcagttaacc ccagctcaag tggtagcaat
      421 cgcgtcgaat ggagggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtctt
      481 gtgccaagat catgggcttc agactcgaac aagcgggcgc tgctcgacgc actggccgaa
      541 gccatgctgg cggagtcgag aatcatagca cttcggtgcc gagagccgac gacgactggc
      601 gctcatttcg agtctgactg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
      661 ccagcagagt ccaatggatc tcgaggaatt cactcctcag gtgcaggctg cctatcagga
      721 gtcccattgt gctggcgcgc tgcaggatac ggtacctctt aattaactgg cctcatgggc
      781 cttccgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaacatgg
      841 tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca aaaggccagg
      961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1861 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     1921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2701 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
