LOCUS       sequence-174632-        3953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB166
FEATURES             Location/Qualifiers
     source          1..3953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2900..2911
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agcttaagtg attagtcaaa gaatggtgat gacaattgta caggttatca ataaagccat
       61 tgatactctc attcttatcc cgtatttatt aacttctcct agtattcgta attatcatgg
      121 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa
      181 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa
      241 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta
      301 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa
      361 aagaccacat tttttaatgt ggtctttatt cttcaactaa agcacccatt agttcaacaa
      421 acgaaaattg gataaagtgg gatattttta aaatatatat ttatgttaca gtaatattga
      481 cttttaaaaa aggattgatt ctaatgaaga aagcagacaa gtaagcctcc taaattcact
      541 ttagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt tagacaattg
      601 gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta gtataaccac
      661 agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata aattttaccc
      721 tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta gaactggtta
      781 caatagcgac ggagagttag gttattggga taagttagag ccactttata caatttttga
      841 tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact tcaaagagtt
      901 ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat tgtttcccaa
      961 aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt catttactgg
     1021 gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta cagcaggaaa
     1081 attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac atcattctgt
     1141 ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt cagataggcc
     1201 taatgactgg cttttataat atgagataat gccgactgta ctttttacag tcggttttct
     1261 aatgtcacta acctgccccg ttagttgaag aaggttttta tattacagct ccagatccat
     1321 atccttcttt ttctgaaccg acttctcctt tttcgcttct ttattccaat tgctttattg
     1381 acgttgagcc tcggaaccct taacaatccc aaaacttgtc gactttcgtc aggggggctt
     1441 ttatttattc aataatccct cctctcaata aatctattgt tgtacttaat tcaacttcca
     1501 tttctctgta tctttcaata cgctctttta agtccttaat ttcttttttt aattcctcat
     1561 tttcagcaaa taactctttt tctttgtttg tcattttatt tcccccgttt cagcatcaag
     1621 aacctttgca taacttgctc tatatccaca ctgataattg ccctcaaacc ataatctaaa
     1681 ggcgctagag tttgttgaaa caatatcttt tacatcattc gtatttaaaa ttccaaactc
     1741 cgctccccta aggcgaataa aagccattaa atcttttgta tttaccaaat tatagtcatc
     1801 cactatatct aaaagtaaat tcttcaattc tcttttttgg ctttcatcaa gtgttatata
     1861 gcggtcaata tcaaaatcat taatgttcaa aatatctttt ttgtcgtata tatgtttatt
     1921 cttagcaata gcgtcctttg attcatgagt caaatattca tatgaacctt tgatataatc
     1981 aagtatctca acatgagcaa ctgaactatt ccccaatttt cgcttaatct tgttcctaac
     2041 gctttctatt gttacaggat ttcgtgcaat atatataacg tgatagtgtg gttttttata
     2101 gtgctttcca tttcgtataa catcactact attccatgta tctttatctt ttttttcgtc
     2161 catatcgtgt aaaggactga cagccataga tacgcccaaa ctctctaatt tttctttcca
     2221 atcattagga attgagtcag gatataataa aaatccaaaa tttctagctt tagtattttt
     2281 aatagccatg atatattacc ttatcaaaaa caagtagcga aaactcgtat ccttctaaaa
     2341 acgcgagctt tcgcttattt tttttgttct gattcctttc ttgcatattc ttctatagct
     2401 aacgccgcaa ccgcagattt tgaaaaacct ttttgtttcg ccatatctgt taatttttta
     2461 tcttgctctt ttgtcagaga aatcataact ctttttttcg attctgaaat caccatttaa
     2521 aaaactccaa tcaaataatt ttataaaatt agtgtatcac tttgtaatca taaaaacaac
     2581 aataaagcta cttaaatata gatttataaa aaacgttggc gaaaacgttg gcgattcgtt
     2641 ggcgattgaa aaacccctca aacccttgag ccagttggga tagagcgttt ttggcacaaa
     2701 aattggcact cggcacttaa tggggggtcg tagtacggaa gcaaaattcg cttcctttcc
     2761 ccccattttt ttccaaattc caaatttttt tcaaaaattt tccagcgcta ccgctcggca
     2821 aaattgcaag caatttttaa aatcaaaccc atgagggaat ttcattccct caaactccct
     2881 tgagcctcct ccaaccgaaa tagaaggacg ctgcgcttat tatttcattc agtcatcggc
     2941 tttcataatc taacagacaa catcttcgct gcaaagcacg ctacgctcaa gggcttttac
     3001 gctacgataa cgcctgtttt aacgattatg ccgataacta aacgaaataa acgctaaaac
     3061 gtctcagaaa cgattttgag acgttttaat aaaaaatcga ccgaaaaata aatataaatc
     3121 ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat
     3181 gaccgatgat gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg
     3241 taaaaggtta atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga
     3301 tgacacagaa gaaggcgatt tgattcatac agatgatgac gaaaaagccg atgaagatgg
     3361 attttctatt attgcaatgt ggaattggga acggaaaaat tattttatta aagagtagtt
     3421 caacaaacgg gccagtttgt tgaagattag atgctataat tgttattaaa aggattgaag
     3481 gatgcttagg aagacgagtt attaatagct gaataagaac ggtgctctcc aaatattctt
     3541 atttagaaaa cgaaatctaa aattatctga aaagggaatg agaatagtga atggaccaat
     3601 aataatgact agagaagaaa gaatgaagat tgttcatgaa attaaggaac gaatattgga
     3661 taaatatggg gatgatgtta aggctattgg tgtttatggc tctcttggtc gtcagactga
     3721 tgggccccga aaagccctga caacccttgt tcctaaaaag gaataagcgt tcggtcagta
     3781 aataatagaa ataaaaaatc agacctaaga ctgatgacaa aaagagaaaa ttttgataaa
     3841 atagtcttag aattaaatta aaaagggagg ccaaatataa tgaaaaatat gaatgacaat
     3901 gatgttggat ccccgcggta cccgggaatt ctagagctcg agatctatcg ata
//
