LOCUS       sequence-174637-        6082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB184 (EM)
FEATURES             Location/Qualifiers
     source          1..6082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3180..3197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3351..3939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3431..3450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taactcaacg ctagtagtgg atttaatccc aaatgagcca acagaaccag agccagaaac
       61 agaatcagaa caagtaacat tggatttaga aatggaagaa gaaaaaagca atgacttcgt
      121 gtgaataatg cacgaaatcg ttgcttattt ttttttaaaa gcggtatact agatataacg
      181 aaacaacgaa ctgaatagaa acgaaaaaag agccatgaca catttataaa atgtttgacg
      241 acattttata aatgcatagc ccgataagat tgccaaacca acgcttatca gttagtcaga
      301 tgaactcttc cctcgtaaga agttatttaa ttaactttgt ttgaagacgg tatataaccg
      361 tactatcatt atatagggaa atcagagagt tttcaagtat ctaagctact gaatttaaga
      421 attgttaagc aatcaatcgg aaatcgtttg attgcttttt ttgtattcat ttatagaagg
      481 tggagtttgt atgaatcatg atgaatgtaa aacttatata aaaaatagtt tattggagat
      541 aagaaaatta gcaaatatct atacactaga aacgtttaag aaagagttag aaaagagaaa
      601 tatctactta gaaacaaaat cagataagta tttttcttcg gagggggaag attatatata
      661 taagttaata gaaaataaca aaataattta ttcgattagt ggaaaaaaat tgacttataa
      721 aggaaaaaaa tctttttcaa aacatgcaat attgaaacag ttgaatgaaa aagcaaacca
      781 agttaattaa acaacctatt ttataggatt tataggaaag gagaacagct gaatgaatat
      841 cccttttgtt gtagaaactg tgcttcatga cggcttgtta aagtacaaat ttaaaaatag
      901 taaaattcgc tcaatcacta ccaagccagg taaaagcaaa ggggctattt ttgcgtatcg
      961 ctcaaaatca agcatgattg gcggtcgtgg tgttgttctg acttccgagg aagcgattca
     1021 agaaaatcaa gatacattta cacattggac acccaacgtt tatcgttatg gaacgtatgc
     1081 agacgaaaac cgttcataca cgaaaggaca ttctgaaaac aatttaagac aaatcaatac
     1141 cttctttatt gattttgata ttcacacggc aaaagaaact atttcagcaa gcgatatttt
     1201 aacaaccgct attgatttag gttttatgcc tactatgatt atcaaatctg ataaaggtta
     1261 tcaagcatat tttgttttag aaacgccagt ctatgtgact tcaaaatcag aatttaaatc
     1321 tgtcaaagca gccaaaataa tttcgcaaaa tatccgagaa tattttggaa agtctttgcc
     1381 agttgatcta acgtgtaatc attttggtat tgctcgcata ccaagaacgg acaatgtaga
     1441 attttttgat cctaattacc gttattcttt caaagaatgg caagattggt ctttcaaaca
     1501 aacagataat aagggcttta ctcgttcaag tctaacggtt ttaagcggta cagaaggcaa
     1561 aaaacaagta gatgaaccct ggtttaatct cttattgcac gaaacgaaat tttcaggaga
     1621 aaagggttta atagggcgta ataacgtcat gtttaccctc tctttagcct actttagttc
     1681 aggctattca atcgaaacgt gcgaatataa tatgtttgag tttaataatc gattagatca
     1741 acccttagaa gaaaaagaag taatcaaaat tgttagaagt gcctattcag aaaactatca
     1801 aggggctaat agggaataca ttaccattct ttgcaaagct tgggtatcaa gtgatttaac
     1861 cagtaaagat ttatttgtcc gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg
     1921 tcaacgtgtt catttgtcag aatggaaaga agatttaatg gcttatatta gcgaaaaaag
     1981 cgatgtatac aagccttatt tagtgacgac caaaaaagag attagagaag tgctaggcat
     2041 tcctgaacgg acattagata aattgctgaa ggtactgaag gcgaatcagg aaattttctt
     2101 taagattaaa ccaggaagaa atggtggcat tcaacttgct agtgttaaat cattgttgct
     2161 atcgatcatt aaagtaaaaa aagaagaaaa agaaagctat ataaaggcgc tgacaaattc
     2221 ttttgactta gagcatacat tcattcaaga gactttaaac aagctagcag aacgccctaa
     2281 aacggacaca caactcgatt tgtttagcta tgatacaggc tgaaaataaa acccgcacta
     2341 tgccattaca tttatatcta tgatacgtgt ttgttttttc tttgctgttt agcgaatgat
     2401 tagcagaaat atacagagta agattttaat taattattag ggggagaagg agagagtagc
     2461 ccgaaaactt ttagttggct tggactgaac gaagtgaggg aaaggctact aaaacgtcga
     2521 ggggcagtga gagcgaagcg aacacttgat tttttaattt tctatctttt ataggtcatt
     2581 agagtatact tatttgtcct ataaactatt tagcagcata atagatttat tgaataggtc
     2641 atttaagttg agcatattag aggaggaaaa tcttggagaa atatttgaag aacccgatta
     2701 catggattgg attagttctt gtggttacgt ggtttttaac taaaagtagt gaatttttga
     2761 tttttggtgt gtgtgtcttg ttgttagtat ttgctagtca aagtgattaa atagaattta
     2821 aggctattgg tgtttatggc tctcttggtc gtcagactga tgggccccga aaagccctga
     2881 caacccttgt tcctaaaaag gaataagcgt tcggtcagta aataatagaa ataaaaaatc
     2941 agacctaaga ctgatgacaa aaagagaaaa ttttgataaa atagtcttag aattaaatta
     3001 aaaagggagg ccaaatataa tgaaaaatat gaatgacaat gatgttggat ccccgcggta
     3061 cccgggaatt ctagagctcg agatctatcg ataagcttaa gtgattagtc aaagaatggt
     3121 gatgacaatt gtactcgacc tgcagccaag cttatcgatt ctagacgctc ttccgcttcc
     3181 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3241 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3781 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4021 tcaaaaagga tcttcaccta gatcctttta aattaaatct agaatcgata cgattttgaa
     4081 gtggcaacag ataaaaaaaa gcagtttaaa attgttgctg aacttttaaa acaagcaaat
     4141 acaatcattg tcgcaacaga tagcgacaga gaaggcgaaa acattgcctg gtcgatcatt
     4201 cataaagcaa atgccttttc taaagataaa acgtataaaa gactatggat caatagttta
     4261 gaaaaagatg tgatccgtag cggttttcaa aatttgcaac caggaatgaa ttactatccc
     4321 ttttatcaag aagcgcaaaa gaaaaacgaa atgatacacc aatcagtgca aaaaaagata
     4381 taatgggaga taagacggtt cgtgttcgtg ctgacttgca ccatatcata aaaatcgaaa
     4441 cagcaaagaa tggcggaaac gtaaaagaag ttatggaaat aagacttaga agcaaactta
     4501 agagtgtgtt gatagtgcag tatcttaaaa ttttgtataa taggaattga agttaaatta
     4561 gatgctaaaa atttgtaatt aagaaggagt gattacatga acaaaaatat aaaatattct
     4621 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
     4681 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
     4741 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
     4801 gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa
     4861 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
     4921 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
     4981 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
     5041 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
     5101 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
     5161 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
     5221 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
     5281 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     5341 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     5401 aggtatacta ctgacagctt ccaaggagct aaagaggtcc ctagcgctct tatcatgggg
     5461 aagctcggat catatgcaag acaaaataaa ctcgcaacag cacttggaga aatgggacga
     5521 atcgagaaaa ccctctttac gctggattac atatctaata aagccgtaag gagacgggtt
     5581 caaaaaggtt taaataaagg agaagcaatc aatgcattag ctagaactat attttttgga
     5641 caacgtggag aatttagaga acgtgctctc caagaccagt tacaaagagc tagtgcacta
     5701 aacataatta ttaacgctat aagtgtgtgg aacactgtat atatggaaaa agccgtagaa
     5761 gaattaaaag caagaggaga atttagagaa gatttaatgc catatgcgtg gccgttagga
     5821 tgggaacata tcaattttct tggagaatac aaatttgaag gattacatga cactgggcaa
     5881 atgaatttac gtcctttacg tataaaagag ccgttttatt cttaatataa cggctctttt
     5941 tatagaaaaa atccttagcg tggttttttt ccgaaatgct ggcggtaccc caagaattag
     6001 aaatgagtag atcaaattat tcacgaatag aatcaggaaa atcagatcca accataaaaa
     6061 cactagaaca aattgcaaag tt
//
