LOCUS       sequence-174639-        6217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB190
FEATURES             Location/Qualifiers
     source          1..6217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3095..3112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3200..3223
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3315..3332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3486..4074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3566..3585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactaactca acgctagtag tggatttaat cccaaatgag ccaacagaac cagagccaga
       61 aacagaatca gaacaagtaa cattggattt agaaatggaa gaagaaaaaa gcaatgactt
      121 cgtgtgaata atgcacgaaa tcgttgctta ttttttttta aaagcggtat actagatata
      181 acgaaacaac gaactgaata gaaacgaaaa aagagccatg acacatttat aaaatgtttg
      241 acgacatttt ataaatgcat agcccgataa gattgccaaa ccaacgctta tcagttagtc
      301 agatgaactc ttccctcgta agaagttatt taattaactt tgtttgaaga cggtatataa
      361 ccgtactatc attatatagg gaaatcagag agttttcaag tatctaagct actgaattta
      421 agaattgtta agcaatcaat cggaaatcgt ttgattgctt tttttgtatt catttataga
      481 aggtggagtt tgtatgaatc atgatgaatg taaaacttat ataaaaaata gtttattgga
      541 gataagaaaa ttagcaaata tctatacact agaaacgttt aagaaagagt tagaaaagag
      601 aaatatctac ttagaaacaa aatcagataa gtatttttct tcggaggggg aagattatat
      661 atataagtta atagaaaata acaaaataat ttattcgatt agtggaaaaa aattgactta
      721 taaaggaaaa aaatcttttt caaaacatgc aatattgaaa cagttgaatg aaaaagcaaa
      781 ccaagttaat taaacaacct attttatagg atttatagga aaggagaaca gctgaatgaa
      841 tatccctttt gttgtagaaa ctgtgcttca tgacggcttg ttaaagtaca aatttaaaaa
      901 tagtaaaatt cgctcaatca ctaccaagcc aggtaaaagc aaaggggcta tttttgcgta
      961 tcgctcaaaa tcaagcatga ttggcggtcg tggtgttgtt ctgacttccg aggaagcgat
     1021 tcaagaaaat caagatacat ttacacattg gacacccaac gtttatcgtt atggaacgta
     1081 tgcagacgaa aaccgttcat acacgaaagg acattctgaa aacaatttaa gacaaatcaa
     1141 taccttcttt attgattttg atattcacac ggcaaaagaa actatttcag caagcgatat
     1201 tttaacaacc gctattgatt taggttttat gcctactatg attatcaaat ctgataaagg
     1261 ttatcaagca tattttgttt tagaaacgcc agtctatgtg acttcaaaat cagaatttaa
     1321 atctgtcaaa gcagccaaaa taatttcgca aaatatccga gaatattttg gaaagtcttt
     1381 gccagttgat ctaacgtgta atcattttgg tattgctcgc ataccaagaa cggacaatgt
     1441 agaatttttt gatcctaatt accgttattc tttcaaagaa tggcaagatt ggtctttcaa
     1501 acaaacagat aataagggct ttactcgttc aagtctaacg gttttaagcg gtacagaagg
     1561 caaaaaacaa gtagatgaac cctggtttaa tctcttattg cacgaaacga aattttcagg
     1621 agaaaagggt ttaatagggc gtaataacgt catgtttacc ctctctttag cctactttag
     1681 ttcaggctat tcaatcgaaa cgtgcgaata taatatgttt gagtttaata atcgattaga
     1741 tcaaccctta gaagaaaaag aagtaatcaa aattgttaga agtgcctatt cagaaaacta
     1801 tcaaggggct aatagggaat acattaccat tctttgcaaa gcttgggtat caagtgattt
     1861 aaccagtaaa gatttatttg tccgtcaagg gtggtttaaa ttcaagaaaa aaagaagcga
     1921 acgtcaacgt gttcatttgt cagaatggaa agaagattta atggcttata ttagcgaaaa
     1981 aagcgatgta tacaagcctt atttagtgac gaccaaaaaa gagattagag aagtgctagg
     2041 cattcctgaa cggacattag ataaattgct gaaggtactg aaggcgaatc aggaaatttt
     2101 ctttaagatt aaaccaggaa gaaatggtgg cattcaactt gctagtgtta aatcattgtt
     2161 gctatcgatc attaaagtaa aaaaagaaga aaaagaaagc tatataaagg cgctgacaaa
     2221 ttcttttgac ttagagcata cattcattca agagacttta aacaagctag cagaacgccc
     2281 taaaacggac acacaactcg atttgtttag ctatgataca ggctgaaaat aaaacccgca
     2341 ctatgccatt acatttatat ctatgatacg tgtttgtttt ttctttgctg tttagcgaat
     2401 gattagcaga aatatacaga gtaagatttt aattaattat tagggggaga aggagagagt
     2461 agcccgaaaa cttttagttg gcttggactg aacgaagtga gggaaaggct actaaaacgt
     2521 cgaggggcag tgagagcgaa gcgaacactt gattttttaa ttttctatct tttataggtc
     2581 attagagtat acttatttgt cctataaact atttagcagc ataatagatt tattgaatag
     2641 gtcatttaag ttgagcatat tagaggagga aaatcttgga gaaatatttg aagaacccga
     2701 ttacatggat tggattagtt cttgtggtta cgtggttttt aactaaaagt agtgaatttt
     2761 tgatttttgg tgtgtgtgtc ttgttgttag tatttgctag tcaaagtgat taaatagaat
     2821 ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc cgaaaagccc
     2881 tgacaaccct tgttcctaaa aaggaataag cgttcggtca gtaaataata gaaataaaaa
     2941 atcagaccta agactgatga caaaaagaga aaattttgat aaaatagtct tagaattaaa
     3001 ttaaaaaggg aggccaaata taatgaaaaa tatgaatgac aatgatgttg gatctcttta
     3061 agaaggagat atacaaatgg ctagcagagg atcgcatcac catcaccatc acggggccgg
     3121 agaccgcggt cccgaattcg agctcggtac ccggggatcc ctcgaggtcg acctgcaggg
     3181 ggaccatggt ctcagcgctt ggagccaccc gcagttcgaa aaataataag cttaagtgat
     3241 tagtcaaaga atggtgatga caattgtact cgacctgcag ccaagcttat cgattctaga
     3301 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3901 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aatctagaat
     4201 cgatacgatt ttgaagtggc aacagataaa aaaaagcagt ttaaaattgt tgctgaactt
     4261 ttaaaacaag caaatacaat cattgtcgca acagatagcg acagagaagg cgaaaacatt
     4321 gcctggtcga tcattcataa agcaaatgcc ttttctaaag ataaaacgta taaaagacta
     4381 tggatcaata gtttagaaaa agatgtgatc cgtagcggtt ttcaaaattt gcaaccagga
     4441 atgaattact atccctttta tcaagaagcg caaaagaaaa acgaaatgat acaccaatca
     4501 gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
     4561 tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac
     4621 ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg tataatagga
     4681 attgaagtta aattagatgc taaaaatttg taattaagaa ggagtgatta catgaacaaa
     4741 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
     4801 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
     4861 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
     4921 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
     4981 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag tattccttac
     5041 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
     5101 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
     5161 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
     5221 cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
     5281 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
     5341 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
     5401 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     5461 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     5521 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctagc
     5581 gctcttatca tggggaagct cggatcatat gcaagacaaa ataaactcgc aacagcactt
     5641 ggagaaatgg gacgaatcga gaaaaccctc tttacgctgg attacatatc taataaagcc
     5701 gtaaggagac gggttcaaaa aggtttaaat aaaggagaag caatcaatgc attagctaga
     5761 actatatttt ttggacaacg tggagaattt agagaacgtg ctctccaaga ccagttacaa
     5821 agagctagtg cactaaacat aattattaac gctataagtg tgtggaacac tgtatatatg
     5881 gaaaaagccg tagaagaatt aaaagcaaga ggagaattta gagaagattt aatgccatat
     5941 gcgtggccgt taggatggga acatatcaat tttcttggag aatacaaatt tgaaggatta
     6001 catgacactg ggcaaatgaa tttacgtcct ttacgtataa aagagccgtt ttattcttaa
     6061 tataacggct ctttttatag aaaaaatcct tagcgtggtt tttttccgaa atgctggcgg
     6121 taccccaaga attagaaatg agtagatcaa attattcacg aatagaatca ggaaaatcag
     6181 atccaaccat aaaaacacta gaacaaattg caaagtt
//
