LOCUS       sequence-174643-        6566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p15A plasmid expressing CasY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Burstein D, Harrington LB, Strutt SC, Probst AJ, Anantharaman K, 
            Thomas BC, Doudna JA, Banfield JF
  TITLE     New CRISPR-Cas systems from uncultivated microbes.
  JOURNAL   Nature. 2016 Dec 22. doi: 10.1038/nature21059.
  PUBMED    28005056
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Dec 22. doi: 10.1038/nature21059."
COMMENT     Sequence Label: pCasY1
FEATURES             Location/Qualifiers
     source          1..6566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2750..2852
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2853..3512
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2919..2938)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3533..3627
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3741..4286
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4187..4206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4403..4420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4448..4475)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gatttgagta caagtcctta tactttttag ttacatcacg atcctctttg cgttcctctt
       61 cagtctggat gtagcgctgg taggacccgt tcagaaggga cttaaaatca ctcaaaataa
      121 aacgatataa ttcaagatca tcggaaaaat ctttttcctt aagcatggtg tccagcagcg
      181 gcagcagcgc ctcacgttga gcctcaatct gctcacgcaa tgaagaatca acgactttct
      241 tcaggtcttc ctgcagcttg tccaaagcga tttgatgttt cttcaagtcc tcacggactt
      301 taatcagttg ctccgtatag tttgaatgcc aactcttaat tttccctccg atgatcatac
      361 gatagtccgc ccaagagctg gtaagcttag ggtagcccag caatttcgct ttttcagata
      421 agaagtgcaa acgtttttct aactcgggtt cgttttccca agacttgctc acagccaaca
      481 cagcggtcac gatttttttg atctccccac gcgaaagcag gttaaaaagc tcgttgaaat
      541 agttgctgaa cgccgaaaag ttgttgtcca gccccaacag ggattcacga ctgatatcaa
      601 acttcttcaa acgttctaaa caattagcta acggcagatg tgggctaaac tcaagactag
      661 gttctacgaa gaatgtcaat tttggaagat catggttatc tttaaaatga gggaagatct
      721 tgataccgaa ttcctcctcc caatacaaaa actgcttaga aactaaaatc gatttccctt
      781 ccacaatcag gtccttggac cacttatcta cacacgcaat aacctcttcg gtgtcacgga
      841 attcttcctt gaaacacttc agaaaacggc cgcgaaggct gcgcacgtta gaggagctgc
      901 gaatgcctgt gttcagaaga atgatgtcct taaactgttc cttgtccagc ttattcttca
      961 attcaaagtc gatctgctca aagattttct ctgaaggtga ctttgatccc tctaacttac
     1021 taattaaatc aatcagacta gtgcttttcg actgccaaat gacgccagca cgtaaagagt
     1081 caatccaaaa atcaactaag gagtaacgat ttgaattacg cgcatacgac gcgacgttca
     1141 gaggaccaaa taagtgctcg taatcataaa taattttctc actcaagtta tttaacgcgg
     1201 tcttatttgg ggcgaccagg ggatacttaa tcgagcggat agccgctttc cctgtgtaaa
     1261 ccaaacgctt gttgtgcagg atgtagcctt tgaacagctt cttacgcatt attaattatc
     1321 aacaaaatca ggaaagggtg gaaggctaaa gtttagttaa aaactggaaa ccctttttat
     1381 tttgatactt tcggagaatg ggaacgctag ttatgccttt atccccgata ctttcggagt
     1441 gtacaacaat aacagtagcc tttatccccg atactttcgg agtaagttca ccagatttat
     1501 gcctttatcc ccgatacttt cggagccaga gtttggtatt gatgccttta tccccgatac
     1561 tttcggagac ttctccccga ttttgttgat attaaaatgt aaacgagcta aaaaccgctt
     1621 gaaaatgtac cttaaatata gcataatttg agacatatat ccaatacgaa atgcgtcgtt
     1681 acttcgcgaa acgtaagcaa tttatttggt tgccttatgt gaagtccatt gacttatcgg
     1741 gcaaatccgt caaattttca tataaaggag gagagcttga tctttccttt ccagagatcc
     1801 actctattat gttctatggg tccgtctgtc cacttgaaca agagttcttg gagaagtgtt
     1861 cggcatgggg aattccatta acaattcacc gtcgcaatat gcctcgcgga gtgtggattg
     1921 tgccaagtac tacatccaat cgcgaggatc tgttgactaa acagattctt ttccgccaaa
     1981 atgagaagaa gcgtgcgtat atcaccaaac gtttgatcta cgccaagttc aaatctatgg
     2041 aatggctgat cccagcgccc actcaggaat tgcaaggact gtcatcggta gagtctatcg
     2101 taggcgtgga gtcatggcat gctaaacgtt attgggaaca cttctacaag tctttagggt
     2161 atccaaaaga tcgccgccgt agtaaaggca attttatctg caatgtactt gacgcctcct
     2221 ccaagttcgt atctggcatc atcctgcgtt gggtggttta ccatcatttt agcccgtttc
     2281 acggttatac acacacgccc agcgattatc catcgttaat ttacgatctg atggaacctt
     2341 atcgtggatt catcgacgga gtagctttca atacaatcaa ggaaatccag gagaatggtg
     2401 gcgacgagtc caagaccttg ccgtacacta tggagaatct taaggacttc atggataccg
     2461 agatttatgt tgccgctact cgtcagacgg cgacgtttca agaactttac cacgggttaa
     2521 ttttgggcct gcgctcctac cttctgggaa ataagcgctt catcgtgcct tttcctggga
     2581 agccgtccgg tggacgtcct gtaaaggctg ggtataagct gtatgggcgc tttgcgggaa
     2641 aaacagattt ctggccggaa tctcgcgata ttgcaaaaac attccaacaa aagtttagta
     2701 tccgcctgga atgaaactat tccgtcgata tctggcgaaa atgagacgtt gatcggcacg
     2761 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
     2821 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2881 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2941 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     3001 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     3061 atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     3121 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3181 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3241 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3301 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3361 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3421 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3481 agtactgcga tgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg
     3541 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     3601 cgccgggcgt tttttattgg tgagaatcca agcactagta acaacttata tcgtatgggg
     3661 ctgacttcag gtgctacatt tgaagagata aattgcactg aaatctagaa atattttatc
     3721 tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa
     3781 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg
     3841 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct
     3901 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag
     3961 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4021 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     4081 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
     4141 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt
     4201 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     4261 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     4321 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     4381 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatccctag
     4441 gtataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagcc aatataatca
     4501 caggaacttt gcagaacgca gcacggcccc gttcttttcc cataacttac ggacctccag
     4561 gatgtaatcc aatttgcgct ccccctcctt tactgcatcc ttagcattat cgctaatgta
     4621 accacggcat gcaatattaa gcgccgcctg gatgtcagca tccgttttgt atccacaaaa
     4681 tgggcaacgg taaatatagc tgttaccacg gcgtttcaac aactgttcta catcttcccc
     4741 ctctaataaa acttcgcgga tcggggggcg ggcatattct ttaatactct taaggacttc
     4801 cttaccctta atagttttgc ccaacaggct cttctgaaga aagccacgca ctttcttttc
     4861 atctccgacg ttgaagataa actcatcacc gcctttttca tattccttat cgttatcgat
     4921 aactaactca aatggcgtgc gagcacaatt gcaacacgta taactagtcg cataagagga
     4981 aatgtgattc cccatctgct tatttttctt tccccagatc atttcgctaa caagcgtgtc
     5041 tgctcccgat tcgcggtaga catccgaaac cttaatactg cggtaaattt tcgccatacg
     5101 gtttccaccg gtttcaaaat tggaaataga aatctcataa ataatcttag ctttataagc
     5161 cagtgcgata ctatgaattt gattacgcag agaatggatt gccatctcgc gaacgcgggc
     5221 tactgcagtg gaggaagacg aaaacactgc catcacttgt ttctttttca tgtcctgtac
     5281 acgctcacgg atcttacgca atgcaggatc cttcaaaaat ccccaactca ataattcaat
     5341 gcggtcgcga acgatgcgaa caaccgcata ggccacccca aattccccca catccaggcc
     5401 cagataagtg ttgcgctgtt cgatttctgc tgattgctct ttataatccg ttgccgggac
     5461 cagattaagc ggcaaagaat agtacaagcg ttcttcgcac gatttttcct tcttcagaag
     5521 cggtaataac ttgtccttgt cccaggaaac gccccactct tcttctaaaa ctaaagacgg
     5581 ttctgataat accaagttca tcaagtccca ctcctttgtc tttttgaata agcggtttaa
     5641 aaactggatt tgatacttcg aggtacgaat gcgccaaatg ttatttttga agatttccac
     5701 ttccttcgcc tttgcaaaat cagttttatc cttgaagatc aggcacttct cctggttctt
     5761 ttcttcaccc aggtcacctt tctcttcgat aagcaccccc cagttgcgcg agaagttctt
     5821 cgtctgtttt tctcccacca agcaaagaag ggggaacttt tggttagagc caatcatttg
     5881 ggtaacgtaa cgaacaacga atttatcgcg agtatacagc gtgatcatcc ccttgatttc
     5941 gctggtaatg cagttaaaga tcatcttact caagtagtaa ccggaaaggc agctcccaaa
     6001 gttcttaatt aaggacgcag cctcagggaa taactttaaa tcatagcttg aaaagtccat
     6061 agagaaatcc ttattacatg acagaagcca gcccagggtc agcttataaa tctcaatcag
     6121 atcaacaacc tcacccgggt ttgagttctt ccaatcaggg gaaatttggt acttatctaa
     6181 taaatagctg atgtccttac ggggattcga gattttgacc ggcagaatac gaatggcgac
     6241 atgagattca cgtgggtaac ggtagaacac ttccgaaatt ttaggcaact cgccattatt
     6301 gtacttgcgc aatacctgtt cggtgatctg tttaaaacgg ttactattga atgcattgat
     6361 tttatattta cgggacaact tctctaattg ttttgtaaca tactcctccg taattgacgg
     6421 aggcttgcgc acggaaactg tctccagtgc gttacggata tcctccaaaa ttttaagacc
     6481 gacgtcaatg gtaggaagac tcttattgat aaatttgttg aattgctctt tcttgctctc
     6541 gccaaagaaa cggggaatat tacgaa
//
