LOCUS       sequence-174737-        8009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains dll4 enhancer 1 sequence, and dll4 specific gene promoter 
            driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;dll4CE1-P1Egfp
FEATURES             Location/Qualifiers
     source          1..8009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1057..1178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1301..2011)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(1381..1404)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1579..1598)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1848..1866
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2008..2017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2659..2675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2695..2715
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3369..3393
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4049..4058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4055..4057,4058..4060,4061..4771)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4055..4057,4058..4060,4061..4771)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4100..4121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4361..4380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4708..4729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4772..4837
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(4857..4881)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4906..4926)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4934..4950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4966..5100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(5539..5726,5727..5738)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5772..5788)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5772..5788)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5825..5845)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5825..5843)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5864..5880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5877..5899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5888..5904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5912..5918,5919..5936,5937..5942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5957..5978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6095..6112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6266..6854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6346..6365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7025..7816,7817..7885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7648..7667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7886..7990)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca
     1321 tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct
     1381 cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg
     1441 gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct
     1501 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
     1561 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
     1621 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
     1681 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     1741 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
     1801 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
     1861 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     1921 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
     1981 ccatgttgtc atcctcgccc ttgctcacca tggtggcgac cggtaatgtc agacctggta
     2041 actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg
     2101 aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
     2161 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
     2221 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
     2281 tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat
     2341 caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga
     2401 acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt
     2461 aaatatcaca gataatcaat cgcttttttt tttcttgact gaaaagctta gactggaaaa
     2521 ttctttgaaa aagtttgcac tacagaaaat aaatacataa catcctgcac tgaatgcaca
     2581 ctattaatag aaaagagagc attattgtag gaaatgtatt gtatatgtac attctgtgca
     2641 tttcacaatt ttctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt
     2701 tgtatagaaa agttgcagct gaacactgaa aaggcagaga aaaggctgcc ctcaggaaca
     2761 gccgaccgtt tttcatgata aggatatttc attgtttttg caacaagaca gcttgagttt
     2821 aaaaaaaaaa aaaaaagtcc tacagccgag cggctcagtt gtttggcggt gatataatgc
     2881 ccgttcagtc tttgttgtgt gggtgtcagt tggcggttgt tgagaggggg ggcatgagta
     2941 gtgttgcccc tttgtttgtt ttcaatggct cataaacagt ctgcataaac cgatagaaaa
     3001 aggccagctt ccccttcgct ctgtgtgcgg ctgattcctc gttcacactc ctcgggtcct
     3061 acgactctgg gattgttttg agctgtccag cgaggaagga agccagtgtg agaaagtggc
     3121 tgtttttttg gagtggacag gatttggcct catcgcgcgc ggttcctctt ttcgtcggct
     3181 gaaacaatgc agtggcgtca cacccctcga ctggtgctcg gcgtttcaca tggaaaaatg
     3241 ttcccagaat tccgctccca ggctcagagg taaggctggg aatggagagc ggaggaaacg
     3301 cagagcgaga gacgaacagg aatggaggaa aagccagaga cctgagcaga actgaacagt
     3361 gctcatatgc aagtttgtac aaaaaagcag gctcgctagg atactaaaat gcgctttcag
     3421 gctgtcccag cttgaaacat gaagaggctc tgctccggaa catcaggtaa ggcatgaccc
     3481 tccactgacc gtgcacgctt tcagtgatgt aatataatta tccgcgcaat taactggaac
     3541 gaggacaaac gacaagtaat aggctgtgcg ttgtgcgaag tttcatccga tttagttttt
     3601 cttcagatac gcgtattctg cactgcatgg tgtgaaatgg tcagtattta atcgtgcctg
     3661 ctttaaatat gccaacagat gctgggcttg ttaataatca ctcgcgctca attggtcatc
     3721 atcatccgcg tagtggtggg tattaaatgt ggatatcatt gttgccaaga gaggggggct
     3781 gactgagcat aagtgggcgg cgtttagcga tagacaagag tttatataat gagcagcgcg
     3841 gacacaaagc cagtataaat actgagggac gtaagaaaca agtcttcatt cacgaattac
     3901 acgagaagcg cgaggatgcc caacagcgca atgctgacgc acaaagttta gaagcaaaac
     3961 aagacataac cattgacact aaacacatcg gtgcgctcac tgtggaatac caaacatgct
     4021 cagtggatat cttgagacga agaggatcgc caccatggtg agcaagggcg aggagctgtt
     4081 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4141 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4201 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4261 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4321 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4381 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4441 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4501 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4561 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4621 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4681 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4741 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
     4801 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatacc
     4861 agctttcttg tacaaagtgg gcgcgcctac gtaactagtc tcgagcaact ttattataca
     4921 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc agacatgata
     4981 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     5041 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     5101 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     5161 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
     5221 tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg
     5281 cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt
     5341 ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt
     5401 ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg
     5461 cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata
     5521 gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
     5581 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
     5641 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
     5701 gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag
     5761 caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
     5821 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     5881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7981 gggttccgcg cacatttccc cgaaaagtg
//
