LOCUS       sequence-174753-       14846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SZT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Wolfson RL, Chantranupong L, Wyant GA, Gu X, Orozco JM, Shen K, 
            Condon KJ, Petri S, Kedir J, Scaria SM, Abu-Remaileh M, Frankel WN, 
            Sabatini DM
  TITLE     KICSTOR recruits GATOR1 to the lysosome and is necessary for 
            nutrients to regulate mTORC1.
  JOURNAL   Nature. 2017 Feb 15. doi: 10.1038/nature21423.
  PUBMED    28199306
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Feb 15. doi: 10.1038/nature21423."
COMMENT     Sequence Label: pRK5-FLAG-SZT2
FEATURES             Location/Qualifiers
     source          1..14846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             924..947
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      11133..11142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    11146..11280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11183..11202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11237..11256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        11349..11706
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11349..11540
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11372..11392)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11557..11692
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11726..11743)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11726..11742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11735..11757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11955..12410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12042..12061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12252..12273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12427..12446)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12546..12568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12606..12624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12692..12796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12797..12865,12866..13657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13015..13034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13828..14416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14317..14336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14570..14587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14704..14725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14740..14745,14746..14763,14764..14770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14778..14794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14783..14805
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14802..14818
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14802..14818
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt caccatggaa atggactaca aggatgacga tgacaagggt ggtggtggtg
      961 gtgcgtcgac gatggcctcg gagcgcccgg agccggaggt ggaagaagct gggcaggtgt
     1021 tcctgttaat gaaaaaggat tatcgaatct cccgaaatgt tcgcctggct tggttcctca
     1081 gtcatctgca ccaaactgtg caggccacac cccaggagat gctgcttcag tctgaacagg
     1141 aattggaagt cctcagtgtc ctgccccctg ggtggcagcc agatgaacca gtggtcccaa
     1201 ggccattcct cctggtacct tccacccggg tcaccttcct ggcttggcag tatcggtttg
     1261 tcattgagtt ggaccttagc ccatctactg gcattgtgga tgattccaca ggggagatct
     1321 tgtttgatga agttttccat gccctgtccc gctgcttagg cgggctgctt cggcccttcc
     1381 gagtgcctgg atcttgcatc gacttccagc ctgagatcta tgtaactatc caggcctact
     1441 cctccatcat tggactgcag tcccaccagg tgctggtaca gggctgcctc ttggaccctt
     1501 cccagcggga ggtgttcctg cagcagatat atgagcagct ctgcctcttt gaggataagg
     1561 tggccaccat gctgcagcag cagtacgatc cccagagcca ggcagaagac cagtccccag
     1621 actcagggga cctactgggc cggaaggtag gcgtctccat ggtgacagct gatcttgggc
     1681 tggtcagtat gattcgtcag ggcatcttgg cactgcagtt actaccctcg aactctagtg
     1741 cagggattat cgtgatcacg gatggggtga ccagtgtacc tgatgttgct gtctgtgaga
     1801 cactgctgaa ccagcttcgc agtggcactg tggcttgttc ctttgtccag gtgggaggag
     1861 tttactctta tgactgcagt tttggccatg tgcccaatgt ggaattaatg aagttcatcg
     1921 caatggcaac atttgggtcc tacctgtcca cttgtcctga gccggagcca ggcaacctgg
     1981 gtctgactgt ctaccaccgg gcatttctcc tctattcctt cctgcgcagt ggggaagcac
     2041 tgaaccctga atattactgc ggctctcagc accgcctatt taatgagcac ctggtctctg
     2101 caagcagcaa ccctgccctg gccttgcgcc ggaagaagca cactgagaag gaggtgccag
     2161 ccgacttggt cagcactgtg tccgtacggc ttcgagaggg ctacagtgtc cgagaggtca
     2221 cactggccaa aggagggtcc caattggagg taaagctggt gctgctgtgg aaacacaaca
     2281 tgcgcattga gtatgtggct atggcaccct ggcccctgga gcctgagggc cctcgagtaa
     2341 cacgggtgga agtgacgatg gaaggcggct acgacatttt gcatgatgtg tcctgtgcac
     2401 taaggcagcc cattcgttca ttgtatcgta cccatgttat ccggcgtttc tggaacacgc
     2461 tgcagagcat caaccagaca gaccagatgc ttgcccacct tcagtccttc tcctcagtgc
     2521 ctgagcattt cacgcttcct gacagcacca agagcggagt gccactcttc tacatccctc
     2581 caggctccac caccccggtg ctctccctcc agcccagtgg ttctgactca tcccatgccc
     2641 agtttgctgc ctactggaag ccagtgctgt ccatggatgc aaattcctgg cagcgatggc
     2701 tgcacatgca tcgcctggtg ctaatcctgg agcatgacac accaatcccc aagcacttgc
     2761 acaccccggg cagcaatggg cgctacagca ctatccagtg caggatctcc cactcctccc
     2821 tgacctctct gctgcgggac tggagcagct tcgtactagt cgagggctat tcttatgtta
     2881 agctgctctc cagtgcccca gaccagcccc ccaattcctt ctacatggtc cgtatcattt
     2941 ccaaggcccc atgcatggtt cttcgcctgg gttttcccat tggcacacca gcaccggccc
     3001 ggcacaagat tgtgtcaggc ttgagggaag agatcctgcg gctgcgtttc ccccaccggg
     3061 tacaaagcaa ggagccaacg cccaaggtga aacgaaaagg gctagggggt gctggtgggg
     3121 gcagctctcc ctccaagtca ccccccgtgc tggggccaca gcaggccctg tctgaccggc
     3181 cctgccttgt ggtcctgcat aagccactgg acaaactgct catcaggtat gagaagctgc
     3241 ccttggacta ccgggcaccc ttcttgctga cattggagcc accaggtcca ctgcccttgg
     3301 tgtcaggccg ctcagcctct tctagcctgg cgtcactgtc ccgctacctc taccatcagc
     3361 gctggctttg gagtgtcccg tcaggactgg cccctgcgct gcctctcagt gccattgccc
     3421 agctcctctc catcctcact gaagtccgac tttctgaagg attccacttc gcctgcagtg
     3481 gggaaggaat catcaacatg gttctggagc ttccaattca gaatgaacca ccagggcagg
     3541 ctgcagctga agagaagcac acctgtgttg tccagtacat cctcttcccc ccacactcta
     3601 cctccaccaa agacagcttc tcgacagatg atgacaatga tgtggaagtg gaggccctgg
     3661 agggagactc agagctcaat ctggtcactg aggtgtgggt ggagccacag tatgggcgag
     3721 tgggacctgg ccctggaatc tggaagcacc tccaggacct gacgtattct gagatcccgc
     3781 aagctctcca cccacgggat gctgcctgca taggctccat gctgagcttt gaatacctga
     3841 tacagctgtg tcagagcaag gaatggggtc ctctgccccc agagccgagg gtctctgatg
     3901 gattggatca gggaggagac acctgcgtcc atgagatccc tttccatttt gacctaatgg
     3961 gattgctgcc acagtgccag cagctccaga tgttcttcct cttgcttgcc agaggtgccg
     4021 aggggccact gctgggggtt catgggatcc cgaaggagca agcagtcggc agcacccagg
     4081 ccacaggaga ctccgctttt acttccctga gtgtaggtct tcctgaaact ctcaagcctc
     4141 tcatctctgc ccagccccct cagtggcgct gctatgcaag gcttgtgaac ccccagcatg
     4201 tgtttctgac ttttctccca gctaccttct cagatgtcca gcgtctggct gcctgtggcc
     4261 tggagggacc ccctcaagag gagacaaagc ctaagtttgg ggattggagt ggggctccca
     4321 gtctgaaaga tctaggagga actgggatca aagctacaaa gtcccacgtc cctgtcctca
     4381 gtgtgaccct ggctagtgac aatgcccaga atcaaggaga gctaagtcca ccattccgtc
     4441 gagacttaca ggcttacgct gggcgtcagg cttcccagac agagagtgcg gatgggcccc
     4501 ggacccggtg tcctgtctac atctacagct gttcactgga agcgctgagg gaacaaatgg
     4561 ttggcatgca gccccctcag gcgccccgag acctcatctt ccggactcag ttcctcgacc
     4621 acccctcccc atcctcagcc tggatggaac cccggtacaa ggaggcagct aaccactgtg
     4681 ccctgctgca ggagcatgca cagcggtgct atgtccgtgg gctattccgc agcttgcagc
     4741 aagcacagag tgtgacctcc caggatttgc tgacagcggt agatgcctgt gaggagctac
     4801 tacaagaaat agacatcacc ccatttctcc ttgcattgtg tggccacact tggggtttgc
     4861 ctcatgcacc cccaagtcct ggtcctctca gccctgggcc cttcagcagc agcatggagg
     4921 agggtgccga acctcgggaa cgagctatcc tagcttctga atccagcata gagaccgagg
     4981 acctaagcga gcctgagttt cagagcaccc gtgtccctgg cattccagac cctgggccag
     5041 agatctctct gacagatgtc tgccagctca gaggagaggc ccatggtgcc cttcatagcg
     5101 tcatccagga gaagttccta gagatcagtc gtctccactt ccgcacagtg ccttccaatc
     5161 cccactactt cttctattgc cctccatcca gcaggcgaga agatgagggg cctcgggaca
     5221 cagtagacag aaaaatcagt gacctggagt tttcagaggc tgagcttatg ggagaagaag
     5281 gagacacatc tgcctgctgt gtggtcactg agagtgaccc agagctagag gtagaatacc
     5341 gggagagccg tgaatcagac ctggggcctg ctgggctaga ctctgcctcg ctgtcagacg
     5401 tagacactgt gaatcctgat gaagactcct tcagtatctt ggggggcgac tcacccactg
     5461 ggcctgagag cttccttcat gacctgccac cgctcttcct gcacctcacg tgctccgtgc
     5521 ggctacgtgg gcagcacagc tcagtacctg tgtgcagcct gcctacctgc ctgggccagg
     5581 tgctttccag tctggagggc cccccagttg gaggccgagt tcccttgagg gacctcagtg
     5641 tgactctgga tgtcttcatg ctgactttgc ccctggaagt ggagctcccc acggcctcgg
     5701 accctcagca ccaccggtca acatctgaaa gcagtgcttc atttccacga tccccagggc
     5761 agccatcatc tttaaggtca gatgatggcc tcgggccccc actgccaccc ccagaagagg
     5821 agaggcaccc aggactatcc aatttggcca cgccccacag actggctatt gagaccacca
     5881 tgaatgagat ccgctggttg ttggaagatg agatggtggg ggcactccga agagggggca
     5941 tcccacagag tcctgccctg caccgcgcag ctgcccatat ccatagttct cctggacgct
     6001 ccacctgcct tcgccaaact ctgccactga gttttgtatt tgggccagag cgttccctca
     6061 cacaattcaa ggaggagttc cgccgcctcc atctccctgg ccatgttctt cttgaagacc
     6121 ctgacagtgg cttcttcttt gtggcagctg gccaacagcc aggtgggtcc catggggagc
     6181 cttcttcagc ggcctgggct tggcacagtc atgaggacag ggctgaaggc atcgaagggg
     6241 agaccctgac agccagcccc caagcacctg ggtccccaga ggattctgag ggtgtccccc
     6301 tcatcagcct gccccgcgtg ccacagggag ggagtcagcc tgggcccagc cggggattaa
     6361 gtctcatgtc cagtcagggc agtgtggact cagaccacct aggttatgat ggtggcagca
     6421 gtggctcaga cagtgagggt cccaatgaca cccttggtga gaaggccccc ttcacattgc
     6481 ggactccacc tgggccagca cctccacagc cttcactctc aggcctccct gggccctgcc
     6541 tgcctgactt ctggctcatt gtccgggtcc tgcaggaccg tgtggaagtg tatgcacatg
     6601 cacggagcct gattcgggag gatggggggc cgggcactga gtgtcgccac ctgcagcagc
     6661 tcctggtgag gcgagttggg gagatctgca gggaggtcaa ccagcgactg cttcttcaag
     6721 accttcatga cagccacgtg tgtaactctc ttctggtggc cgagagtgaa gaagatctgt
     6781 ggcgcagtga gactcccttc cactcccgtc agcgggcacc actgcccagt gatgattatg
     6841 ctgctgatga gagctgtgcg ccccgtgggt acctggcagc cacaatgcag tttgtccctg
     6901 gccatttctc ctgtgacgtt gtgtggggaa ctgtgatccg agtccattca cgcctcaaaa
     6961 tggggcccag catgggggtc tctcgggcca tccaggccct gcgctctgtg ctcaatgcct
     7021 tctctgtggt gaaccggaaa aacatgtttg tttaccagga gcgagcaaca aaggctgtgt
     7081 actatcttcg gctcctagag acatcctgca gtgaccggcc atggaaaggg gatgcgctgc
     7141 ccccttccct cgctctgtcc cgaagccaag agcccatcta ctctgaggaa gcctcgggtc
     7201 ctcgttctcc cttagacatg gtctctagcc gcagttcaga tgctgctcgt cctgtgggcc
     7261 aagtggacag acatatccag ctgctggtcc atggtgttgg gcaggcaggt cctgagatca
     7321 cggatgagct cgtgcgagtt ctatgtcggc gcctggatga ggccacgctg gacgtcatca
     7381 cagttatgct tgttcggaac tgcaagctga caccagctga tgtggagttc atccagcccc
     7441 ctggaagtct cccctcagag gtgctgcatc tggccctacc cacctcctgc aggccctggc
     7501 ttccagccct ggcatggtac ctacggcaga acttgctcat cttcctgcac tctcccaagt
     7561 acacagatag caacagccgg aaccacttcc aacatccact cccaccacag ggtggcctcc
     7621 ctgacttgga catctacttg tataacaagc ctggtggaca gggcactggg ggcaaagggg
     7681 ttgcctgcat cactctagcc tttgtggatg aaggaggggc ccccttgtca ctggcgttgt
     7741 ggcccccctc ctctccgggg cccccagacc cactgcgaga ggaggaattt gagcaactga
     7801 cccaggtcat ccgctgcccg gttgttgtgg acagttcttc agctcagaat ggggccccac
     7861 ggcttcgatt ggatgtgtgg gaaaagggga acattagtat tgtgcagctg gaggagaaac
     7921 tccgaggagc agctcgccag gccctggccg atgccatcat cgagcttcag ctgctgccag
     7981 cttcactatg tacagaggac acacccacag gaagtctcag gaacggatcg ttggaaacta
     8041 agagctctgc aggccgagct agcacctttc cccctgcccc tgtccctggg gagcctgtga
     8101 ctccacccag caaagcgggc cggcgtagct tctgggatat gctgagtaaa acagaatgtg
     8161 gggatttggg ttcccccaaa acaactgatg acattgtcct ggatcggcca gaagacactc
     8221 ggggccggag gcgtcacaaa accgagagtg ttcggactcc tggtggagct gagcgggcgc
     8281 caggctcaga ttctggagcc cagagacaaa agcgccggac aacacagcta gaagagggtg
     8341 aggtggggac ccttcatcct gtgtttgccc gtgttgctca gcgctggatg gagtttatgg
     8401 ttcagattgg ttgtgcctca gtgtccagaa gctctgccca catggtgtcc cggttcctcc
     8461 ttccatccat cctgtctgag ttcaccgcac tggtcacctc aatggctgga gacaccagtg
     8521 tccgcatctt tgagcagcat ttgggttcag agccagagat cttcggccct tgttcccctg
     8581 ggcaactggg cccctctccc cgccctgcag ctgagcggca tctgctgctt ctgggaagga
     8641 acttcttgca gtggaggaga ccaacacagc aggctgccaa agccatgcag cgcttcgagc
     8701 caggaggtga tgggagctca gggcgaaatg ctccccggca gaggctcttg ctactagagg
     8761 ttgtggacaa gaagctacag ctgctgacct acaactgggc tccagacctg ggggcagcat
     8821 tgggccgagc gctggttcgc ctggtgcagt ggcagaatgc acgagcccat ctcatcttct
     8881 gcctactcag ccagaagctt ggcctcttcc atcattatgg ccagttggac ttccccgtgc
     8941 gagatgaaaa ggagccaaac ccattcctgc tgccgaccat ggaagtggag accctcatcc
     9001 ggagtgcaag tcccccgctg agccgtgagc agggccgact gagtgggtcc tctcgtggtg
     9061 ggggtcctct tcccctggac acattcccct ttgacgaggc cctaagggat atcacggctg
     9121 cccgccccag ctccgtactt ggtcctgtgc ccagacctcc tgatcctgtc acctaccatg
     9181 gacaacagtt cctagagatc aagatggcag agcgcagaga gctggagcgc cagatgaaga
     9241 tggaaaacct gtttgtaacc tggcagcagc gttctacccc agccaccatg cccatcagtg
     9301 ctggagagct ggagaccctg aagcagtcat cccgcctggt gcattactgt gcaacagcca
     9361 tgctcttcga cccagctgcc tggctgcatg ggcccccaga gacctctgga ccccctgacg
     9421 ggcagcggcg ccatcgccct gagtcagggt ctgggagccg agaggccccc acaagctgtg
     9481 aatccttgga tgtgtcgccc ccgggagccc gtgaggagcc ttggctgaag gagctgagct
     9541 tggctttcct gcagcaatat gtgcagtatc tgcagagcat aggttttgtg ctggtaccac
     9601 tgcggccccc ctcacccgcc cgcagcacca gccggccacg ggccatggct atccttggaa
     9661 cagagggtcg aggctccttc tcctgcccta aaaccaagac tgatgggagc cccaagagca
     9721 ctagctctcc ggtaaccacc taccacctgc agcgggcact gcctgggggc atcatcctca
     9781 tggaactggc attccagggc tgttacttct gtgtcaaaca gtttgccctg gaatgttccc
     9841 gaatcccaat ggggcaggct gtcaactcac agctgtccat gctgttcaca gaggagtgtg
     9901 acaaggtgcg ggacctgatg cacgtgcact cgttcagcta tgacttccat ctgcgcctcg
     9961 tgcatcagca cgtgctaggt gcccatctgg tgctgcggca cggctaccac ctcaccacct
    10021 ttctgcgaca cttcctggcc caccaccctg acggacccca ctttggccgc aatcacattt
    10081 accaagggac attggagctc cccacaccac tcattgctgc ccaccagcta tacaactacg
    10141 tggctgatca cgccagctct taccacatga agccattgcg aatggcccgg ccagggggcc
    10201 cagaacacaa cgagtatgcc ctggtgtcgg catggcacag ttctggctcc tacctggact
    10261 ctgagggact tcgacaccag gatgactttg atgtgtctct gcttgtctgt cactgtgctg
    10321 caccctttga ggagcaagga gaggctgagc ggcacgttct gcggctacag ttcttcgtgg
    10381 tgctcaccag ccagcgagag ctcttcccca ggctcactgc tgacatgcgc cgcttccgga
    10441 agccacccag actgccccct gagccagagg ctcctgggag ttcagctggc agccctgggg
    10501 aggcctcagg gcttattcta gcgcctggac cggctcctct gttcccacca ctggctgcag
    10561 aggtgggcat ggcacgagca cggctggctc agctggtgcg gctggctgga gggcactgcc
    10621 gtcgggacac cctttggaag cgcctcttct tgctggagcc accggggcct gatcgactgc
    10681 ggctaggggg gcgcctggcc ctggcagagc tggaggaact cctagaagca gtccatgcca
    10741 aatccattgg ggacatcgac ccccagctgg actgcttcct atccatgacg gtctcctggt
    10801 accagagcct gatcaaagtt ctcctaagcc gcttccccca gagctgtcgc catttccaaa
    10861 gcccagactt gggaactcag tacctggttg tgctgaatca gaagttcact gactgctttg
    10921 tgctagtgtt tctggactcc cacttaggaa agacgtctct gacagtggtt ttccgagagc
    10981 ccttcccagt acagccccag gacagcgaga gcccccctgc ccaactggtc tccacctacc
    11041 accacctgga gtctgtcatc aacacagcct gtttcaccct ctggacccgc ctcctctgag
    11101 cggccgctaa gtaagtaagg atccccagct tggccgccat ggcccaactt gtttattgca
    11161 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11221 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11281 gggaattaat tcggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
    11341 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
    11401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    11461 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    11521 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    11581 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    11641 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    11701 tgcaaaaagc tgttaacagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    11761 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    11821 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    11881 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
    11941 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    12001 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    12061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    12121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
    12181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    12241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
    12301 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    12361 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
    12421 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
    12481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    12541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
    12601 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    12661 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    12721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    12781 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    12841 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    12901 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    12961 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    13021 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    13081 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    13141 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    13201 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    13261 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    13321 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    13381 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    13441 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    13501 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    13561 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    13621 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    13681 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    13741 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    13801 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    13861 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    13921 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    13981 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    14041 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    14101 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    14161 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    14221 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    14281 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    14341 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    14401 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    14461 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    14521 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    14581 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    14641 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    14701 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    14761 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    14821 gattacgaat taattcgagc tcgccc
//
