LOCUS       sequence-174755-       14893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SZT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14893)
  AUTHORS   Wolfson RL, Chantranupong L, Wyant GA, Gu X, Orozco JM, Shen K, 
            Condon KJ, Petri S, Kedir J, Scaria SM, Abu-Remaileh M, Frankel WN, 
            Sabatini DM
  TITLE     KICSTOR recruits GATOR1 to the lysosome and is necessary for 
            nutrients to regulate mTORC1.
  JOURNAL   Nature. 2017 Feb 15. doi: 10.1038/nature21423.
  PUBMED    28199306
REFERENCE   2  (bases 1 to 14893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Feb 15. doi: 10.1038/nature21423."
COMMENT     Sequence Label: pRK5-HA-SZT2
FEATURES             Location/Qualifiers
     source          1..14893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      950..959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             962..988
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      11180..11189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    11193..11327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11230..11249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11284..11303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        11396..11753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11396..11587
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11419..11439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11604..11739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11773..11790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11773..11789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11782..11804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12002..12457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12089..12108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12299..12320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12474..12493)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12593..12615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12653..12671)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12739..12843
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12844..12912,12913..13704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13062..13081)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13875..14463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14364..14383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14617..14634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14751..14772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14787..14792,14793..14810,14811..14817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14825..14841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14830..14852
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14849..14865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14849..14865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt cccgaaccga cagtcggtct cttcaccaag gccattcgcg ccaccatggg
      961 ctacccctat gatgtgcctg actacgcaga tctcaatggt ggtggtggtg ggtcgacgat
     1021 ggcctcggag cgcccggagc cggaggtgga agaagctggg caggtgttcc tgttaatgaa
     1081 aaaggattat cgaatctccc gaaatgttcg cctggcttgg ttcctcagtc atctgcacca
     1141 aactgtgcag gccacacccc aggagatgct gcttcagtct gaacaggaat tggaagtcct
     1201 cagtgtcctg ccccctgggt ggcagccaga tgaaccagtg gtcccaaggc cattcctcct
     1261 ggtaccttcc acccgggtca ccttcctggc ttggcagtat cggtttgtca ttgagttgga
     1321 ccttagccca tctactggca ttgtggatga ttccacaggg gagatcttgt ttgatgaagt
     1381 tttccatgcc ctgtcccgct gcttaggcgg gctgcttcgg cccttccgag tgcctggatc
     1441 ttgcatcgac ttccagcctg agatctatgt aactatccag gcctactcct ccatcattgg
     1501 actgcagtcc caccaggtgc tggtacaggg ctgcctcttg gacccttccc agcgggaggt
     1561 gttcctgcag cagatatatg agcagctctg cctctttgag gataaggtgg ccaccatgct
     1621 gcagcagcag tacgatcccc agagccaggc agaagaccag tccccagact caggggacct
     1681 actgggccgg aaggtaggcg tctccatggt gacagctgat cttgggctgg tcagtatgat
     1741 tcgtcagggc atcttggcac tgcagttact accctcgaac tctagtgcag ggattatcgt
     1801 gatcacggat ggggtgacca gtgtacctga tgttgctgtc tgtgagacac tgctgaacca
     1861 gcttcgcagt ggcactgtgg cttgttcctt tgtccaggtg ggaggagttt actcttatga
     1921 ctgcagtttt ggccatgtgc ccaatgtgga attaatgaag ttcatcgcaa tggcaacatt
     1981 tgggtcctac ctgtccactt gtcctgagcc ggagccaggc aacctgggtc tgactgtcta
     2041 ccaccgggca tttctcctct attccttcct gcgcagtggg gaagcactga accctgaata
     2101 ttactgcggc tctcagcacc gcctatttaa tgagcacctg gtctctgcaa gcagcaaccc
     2161 tgccctggcc ttgcgccgga agaagcacac tgagaaggag gtgccagccg acttggtcag
     2221 cactgtgtcc gtacggcttc gagagggcta cagtgtccga gaggtcacac tggccaaagg
     2281 agggtcccaa ttggaggtaa agctggtgct gctgtggaaa cacaacatgc gcattgagta
     2341 tgtggctatg gcaccctggc ccctggagcc tgagggccct cgagtaacac gggtggaagt
     2401 gacgatggaa ggcggctacg acattttgca tgatgtgtcc tgtgcactaa ggcagcccat
     2461 tcgttcattg tatcgtaccc atgttatccg gcgtttctgg aacacgctgc agagcatcaa
     2521 ccagacagac cagatgcttg cccaccttca gtccttctcc tcagtgcctg agcatttcac
     2581 gcttcctgac agcaccaaga gcggagtgcc actcttctac atccctccag gctccaccac
     2641 cccggtgctc tccctccagc ccagtggttc tgactcatcc catgcccagt ttgctgccta
     2701 ctggaagcca gtgctgtcca tggatgcaaa ttcctggcag cgatggctgc acatgcatcg
     2761 cctggtgcta atcctggagc atgacacacc aatccccaag cacttgcaca ccccgggcag
     2821 caatgggcgc tacagcacta tccagtgcag gatctcccac tcctccctga cctctctgct
     2881 gcgggactgg agcagcttcg tactagtcga gggctattct tatgttaagc tgctctccag
     2941 tgccccagac cagcccccca attccttcta catggtccgt atcatttcca aggccccatg
     3001 catggttctt cgcctgggtt ttcccattgg cacaccagca ccggcccggc acaagattgt
     3061 gtcaggcttg agggaagaga tcctgcggct gcgtttcccc caccgggtac aaagcaagga
     3121 gccaacgccc aaggtgaaac gaaaagggct agggggtgct ggtgggggca gctctccctc
     3181 caagtcaccc cccgtgctgg ggccacagca ggccctgtct gaccggccct gccttgtggt
     3241 cctgcataag ccactggaca aactgctcat caggtatgag aagctgccct tggactaccg
     3301 ggcacccttc ttgctgacat tggagccacc aggtccactg cccttggtgt caggccgctc
     3361 agcctcttct agcctggcgt cactgtcccg ctacctctac catcagcgct ggctttggag
     3421 tgtcccgtca ggactggccc ctgcgctgcc tctcagtgcc attgcccagc tcctctccat
     3481 cctcactgaa gtccgacttt ctgaaggatt ccacttcgcc tgcagtgggg aaggaatcat
     3541 caacatggtt ctggagcttc caattcagaa tgaaccacca gggcaggctg cagctgaaga
     3601 gaagcacacc tgtgttgtcc agtacatcct cttcccccca cactctacct ccaccaaaga
     3661 cagcttctcg acagatgatg acaatgatgt ggaagtggag gccctggagg gagactcaga
     3721 gctcaatctg gtcactgagg tgtgggtgga gccacagtat gggcgagtgg gacctggccc
     3781 tggaatctgg aagcacctcc aggacctgac gtattctgag atcccgcaag ctctccaccc
     3841 acgggatgct gcctgcatag gctccatgct gagctttgaa tacctgatac agctgtgtca
     3901 gagcaaggaa tggggtcctc tgcccccaga gccgagggtc tctgatggat tggatcaggg
     3961 aggagacacc tgcgtccatg agatcccttt ccattttgac ctaatgggat tgctgccaca
     4021 gtgccagcag ctccagatgt tcttcctctt gcttgccaga ggtgccgagg ggccactgct
     4081 gggggttcat gggatcccga aggagcaagc agtcggcagc acccaggcca caggagactc
     4141 cgcttttact tccctgagtg taggtcttcc tgaaactctc aagcctctca tctctgccca
     4201 gccccctcag tggcgctgct atgcaaggct tgtgaacccc cagcatgtgt ttctgacttt
     4261 tctcccagct accttctcag atgtccagcg tctggctgcc tgtggcctgg agggaccccc
     4321 tcaagaggag acaaagccta agtttgggga ttggagtggg gctcccagtc tgaaagatct
     4381 aggaggaact gggatcaaag ctacaaagtc ccacgtccct gtcctcagtg tgaccctggc
     4441 tagtgacaat gcccagaatc aaggagagct aagtccacca ttccgtcgag acttacaggc
     4501 ttacgctggg cgtcaggctt cccagacaga gagtgcggat gggccccgga cccggtgtcc
     4561 tgtctacatc tacagctgtt cactggaagc gctgagggaa caaatggttg gcatgcagcc
     4621 ccctcaggcg ccccgagacc tcatcttccg gactcagttc ctcgaccacc cctccccatc
     4681 ctcagcctgg atggaacccc ggtacaagga ggcagctaac cactgtgccc tgctgcagga
     4741 gcatgcacag cggtgctatg tccgtgggct attccgcagc ttgcagcaag cacagagtgt
     4801 gacctcccag gatttgctga cagcggtaga tgcctgtgag gagctactac aagaaataga
     4861 catcacccca tttctccttg cattgtgtgg ccacacttgg ggtttgcctc atgcaccccc
     4921 aagtcctggt cctctcagcc ctgggccctt cagcagcagc atggaggagg gtgccgaacc
     4981 tcgggaacga gctatcctag cttctgaatc cagcatagag accgaggacc taagcgagcc
     5041 tgagtttcag agcacccgtg tccctggcat tccagaccct gggccagaga tctctctgac
     5101 agatgtctgc cagctcagag gagaggccca tggtgccctt catagcgtca tccaggagaa
     5161 gttcctagag atcagtcgtc tccacttccg cacagtgcct tccaatcccc actacttctt
     5221 ctattgccct ccatccagca ggcgagaaga tgaggggcct cgggacacag tagacagaaa
     5281 aatcagtgac ctggagtttt cagaggctga gcttatggga gaagaaggag acacatctgc
     5341 ctgctgtgtg gtcactgaga gtgacccaga gctagaggta gaataccggg agagccgtga
     5401 atcagacctg gggcctgctg ggctagactc tgcctcgctg tcagacgtag acactgtgaa
     5461 tcctgatgaa gactccttca gtatcttggg gggcgactca cccactgggc ctgagagctt
     5521 ccttcatgac ctgccaccgc tcttcctgca cctcacgtgc tccgtgcggc tacgtgggca
     5581 gcacagctca gtacctgtgt gcagcctgcc tacctgcctg ggccaggtgc tttccagtct
     5641 ggagggcccc ccagttggag gccgagttcc cttgagggac ctcagtgtga ctctggatgt
     5701 cttcatgctg actttgcccc tggaagtgga gctccccacg gcctcggacc ctcagcacca
     5761 ccggtcaaca tctgaaagca gtgcttcatt tccacgatcc ccagggcagc catcatcttt
     5821 aaggtcagat gatggcctcg ggcccccact gccaccccca gaagaggaga ggcacccagg
     5881 actatccaat ttggccacgc cccacagact ggctattgag accaccatga atgagatccg
     5941 ctggttgttg gaagatgaga tggtgggggc actccgaaga gggggcatcc cacagagtcc
     6001 tgccctgcac cgcgcagctg cccatatcca tagttctcct ggacgctcca cctgccttcg
     6061 ccaaactctg ccactgagtt ttgtatttgg gccagagcgt tccctcacac aattcaagga
     6121 ggagttccgc cgcctccatc tccctggcca tgttcttctt gaagaccctg acagtggctt
     6181 cttctttgtg gcagctggcc aacagccagg tgggtcccat ggggagcctt cttcagcggc
     6241 ctgggcttgg cacagtcatg aggacagggc tgaaggcatc gaaggggaga ccctgacagc
     6301 cagcccccaa gcacctgggt ccccagagga ttctgagggt gtccccctca tcagcctgcc
     6361 ccgcgtgcca cagggaggga gtcagcctgg gcccagccgg ggattaagtc tcatgtccag
     6421 tcagggcagt gtggactcag accacctagg ttatgatggt ggcagcagtg gctcagacag
     6481 tgagggtccc aatgacaccc ttggtgagaa ggcccccttc acattgcgga ctccacctgg
     6541 gccagcacct ccacagcctt cactctcagg cctccctggg ccctgcctgc ctgacttctg
     6601 gctcattgtc cgggtcctgc aggaccgtgt ggaagtgtat gcacatgcac ggagcctgat
     6661 tcgggaggat ggggggccgg gcactgagtg tcgccacctg cagcagctcc tggtgaggcg
     6721 agttggggag atctgcaggg aggtcaacca gcgactgctt cttcaagacc ttcatgacag
     6781 ccacgtgtgt aactctcttc tggtggccga gagtgaagaa gatctgtggc gcagtgagac
     6841 tcccttccac tcccgtcagc gggcaccact gcccagtgat gattatgctg ctgatgagag
     6901 ctgtgcgccc cgtgggtacc tggcagccac aatgcagttt gtccctggcc atttctcctg
     6961 tgacgttgtg tggggaactg tgatccgagt ccattcacgc ctcaaaatgg ggcccagcat
     7021 gggggtctct cgggccatcc aggccctgcg ctctgtgctc aatgccttct ctgtggtgaa
     7081 ccggaaaaac atgtttgttt accaggagcg agcaacaaag gctgtgtact atcttcggct
     7141 cctagagaca tcctgcagtg accggccatg gaaaggggat gcgctgcccc cttccctcgc
     7201 tctgtcccga agccaagagc ccatctactc tgaggaagcc tcgggtcctc gttctccctt
     7261 agacatggtc tctagccgca gttcagatgc tgctcgtcct gtgggccaag tggacagaca
     7321 tatccagctg ctggtccatg gtgttgggca ggcaggtcct gagatcacgg atgagctcgt
     7381 gcgagttcta tgtcggcgcc tggatgaggc cacgctggac gtcatcacag ttatgcttgt
     7441 tcggaactgc aagctgacac cagctgatgt ggagttcatc cagccccctg gaagtctccc
     7501 ctcagaggtg ctgcatctgg ccctacccac ctcctgcagg ccctggcttc cagccctggc
     7561 atggtaccta cggcagaact tgctcatctt cctgcactct cccaagtaca cagatagcaa
     7621 cagccggaac cacttccaac atccactccc accacagggt ggcctccctg acttggacat
     7681 ctacttgtat aacaagcctg gtggacaggg cactgggggc aaaggggttg cctgcatcac
     7741 tctagccttt gtggatgaag gaggggcccc cttgtcactg gcgttgtggc ccccctcctc
     7801 tccggggccc ccagacccac tgcgagagga ggaatttgag caactgaccc aggtcatccg
     7861 ctgcccggtt gttgtggaca gttcttcagc tcagaatggg gccccacggc ttcgattgga
     7921 tgtgtgggaa aaggggaaca ttagtattgt gcagctggag gagaaactcc gaggagcagc
     7981 tcgccaggcc ctggccgatg ccatcatcga gcttcagctg ctgccagctt cactatgtac
     8041 agaggacaca cccacaggaa gtctcaggaa cggatcgttg gaaactaaga gctctgcagg
     8101 ccgagctagc acctttcccc ctgcccctgt ccctggggag cctgtgactc cacccagcaa
     8161 agcgggccgg cgtagcttct gggatatgct gagtaaaaca gaatgtgggg atttgggttc
     8221 ccccaaaaca actgatgaca ttgtcctgga tcggccagaa gacactcggg gccggaggcg
     8281 tcacaaaacc gagagtgttc ggactcctgg tggagctgag cgggcgccag gctcagattc
     8341 tggagcccag agacaaaagc gccggacaac acagctagaa gagggtgagg tggggaccct
     8401 tcatcctgtg tttgcccgtg ttgctcagcg ctggatggag tttatggttc agattggttg
     8461 tgcctcagtg tccagaagct ctgcccacat ggtgtcccgg ttcctccttc catccatcct
     8521 gtctgagttc accgcactgg tcacctcaat ggctggagac accagtgtcc gcatctttga
     8581 gcagcatttg ggttcagagc cagagatctt cggcccttgt tcccctgggc aactgggccc
     8641 ctctccccgc cctgcagctg agcggcatct gctgcttctg ggaaggaact tcttgcagtg
     8701 gaggagacca acacagcagg ctgccaaagc catgcagcgc ttcgagccag gaggtgatgg
     8761 gagctcaggg cgaaatgctc cccggcagag gctcttgcta ctagaggttg tggacaagaa
     8821 gctacagctg ctgacctaca actgggctcc agacctgggg gcagcattgg gccgagcgct
     8881 ggttcgcctg gtgcagtggc agaatgcacg agcccatctc atcttctgcc tactcagcca
     8941 gaagcttggc ctcttccatc attatggcca gttggacttc cccgtgcgag atgaaaagga
     9001 gccaaaccca ttcctgctgc cgaccatgga agtggagacc ctcatccgga gtgcaagtcc
     9061 cccgctgagc cgtgagcagg gccgactgag tgggtcctct cgtggtgggg gtcctcttcc
     9121 cctggacaca ttcccctttg acgaggccct aagggatatc acggctgccc gccccagctc
     9181 cgtacttggt cctgtgccca gacctcctga tcctgtcacc taccatggac aacagttcct
     9241 agagatcaag atggcagagc gcagagagct ggagcgccag atgaagatgg aaaacctgtt
     9301 tgtaacctgg cagcagcgtt ctaccccagc caccatgccc atcagtgctg gagagctgga
     9361 gaccctgaag cagtcatccc gcctggtgca ttactgtgca acagccatgc tcttcgaccc
     9421 agctgcctgg ctgcatgggc ccccagagac ctctggaccc cctgacgggc agcggcgcca
     9481 tcgccctgag tcagggtctg ggagccgaga ggcccccaca agctgtgaat ccttggatgt
     9541 gtcgcccccg ggagcccgtg aggagccttg gctgaaggag ctgagcttgg ctttcctgca
     9601 gcaatatgtg cagtatctgc agagcatagg ttttgtgctg gtaccactgc ggcccccctc
     9661 acccgcccgc agcaccagcc ggccacgggc catggctatc cttggaacag agggtcgagg
     9721 ctccttctcc tgccctaaaa ccaagactga tgggagcccc aagagcacta gctctccggt
     9781 aaccacctac cacctgcagc gggcactgcc tgggggcatc atcctcatgg aactggcatt
     9841 ccagggctgt tacttctgtg tcaaacagtt tgccctggaa tgttcccgaa tcccaatggg
     9901 gcaggctgtc aactcacagc tgtccatgct gttcacagag gagtgtgaca aggtgcggga
     9961 cctgatgcac gtgcactcgt tcagctatga cttccatctg cgcctcgtgc atcagcacgt
    10021 gctaggtgcc catctggtgc tgcggcacgg ctaccacctc accacctttc tgcgacactt
    10081 cctggcccac caccctgacg gaccccactt tggccgcaat cacatttacc aagggacatt
    10141 ggagctcccc acaccactca ttgctgccca ccagctatac aactacgtgg ctgatcacgc
    10201 cagctcttac cacatgaagc cattgcgaat ggcccggcca gggggcccag aacacaacga
    10261 gtatgccctg gtgtcggcat ggcacagttc tggctcctac ctggactctg agggacttcg
    10321 acaccaggat gactttgatg tgtctctgct tgtctgtcac tgtgctgcac cctttgagga
    10381 gcaaggagag gctgagcggc acgttctgcg gctacagttc ttcgtggtgc tcaccagcca
    10441 gcgagagctc ttccccaggc tcactgctga catgcgccgc ttccggaagc cacccagact
    10501 gccccctgag ccagaggctc ctgggagttc agctggcagc cctggggagg cctcagggct
    10561 tattctagcg cctggaccgg ctcctctgtt cccaccactg gctgcagagg tgggcatggc
    10621 acgagcacgg ctggctcagc tggtgcggct ggctggaggg cactgccgtc gggacaccct
    10681 ttggaagcgc ctcttcttgc tggagccacc ggggcctgat cgactgcggc taggggggcg
    10741 cctggccctg gcagagctgg aggaactcct agaagcagtc catgccaaat ccattgggga
    10801 catcgacccc cagctggact gcttcctatc catgacggtc tcctggtacc agagcctgat
    10861 caaagttctc ctaagccgct tcccccagag ctgtcgccat ttccaaagcc cagacttggg
    10921 aactcagtac ctggttgtgc tgaatcagaa gttcactgac tgctttgtgc tagtgtttct
    10981 ggactcccac ttaggaaaga cgtctctgac agtggttttc cgagagccct tcccagtaca
    11041 gccccaggac agcgagagcc cccctgccca actggtctcc acctaccacc acctggagtc
    11101 tgtcatcaac acagcctgtt tcaccctctg gacccgcctc ctctgagcgg ccgctaagta
    11161 agtaaggatc cccagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt
    11221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg
    11341 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
    11401 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    11461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    11521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    11581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    11641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt
    11761 taacagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    11821 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11881 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
    11941 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
    12001 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    12061 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    12121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    12181 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
    12241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    12301 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
    12361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    12421 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
    12481 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    12541 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    12601 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    12661 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    12721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    12781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    12841 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    12901 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    12961 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    13021 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    13081 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    13141 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    13201 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    13261 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    13321 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    13381 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    13441 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    13501 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    13561 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    13621 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    13681 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    13741 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    13801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    13861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13981 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    14041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    14101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    14161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    14221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    14281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    14341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    14401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    14461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    14521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    14581 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    14641 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    14701 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    14761 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    14821 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattaa
    14881 ttcgagctcg ccc
//
