LOCUS       sequence-174810-       10811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To convert human skin fibroblasts into induced motor neurons (hiMN) 
            in combination with NGN2, Sox11, FGF2 and two small molecules, 
            forskolin and dorsomorphin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Liu ML, Zang T, Zhang CL
  TITLE     Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of 
            Motor Neurons from Human ALS Patients.
  JOURNAL   Cell Rep. 2016 Jan 5;14(1):115-28. doi: 
            10.1016/j.celrep.2015.12.018. Epub 2015 Dec 24.
  PUBMED    26725112
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.12.018"; journalName: "Cell Rep"; date: 
            "2016-01-5- 5"; volume: "14"; issue: "1"; pages: "115-28"
COMMENT     Sequence Label: pCSC-ISL1-T2A-LHX3
FEATURES             Location/Qualifiers
     source          1..10811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2528..2831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2832..3035
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3032..3056
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3124..3133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3920..3929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4204..4257
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4264..4273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5617..6205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5670..5690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6088..6099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6612..6792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6818..6835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6824..7048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7094..7522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7181..7200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7391..7412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7531..7551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7536..7866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7717..7852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7914..7961
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7980..8354
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8484..8605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8521..8540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8575..8594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8654..8670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8654..8670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8667..8689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8678..8694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8702..8708,8709..8726,8727..8732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8747..8768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8885..8902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9056..9644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9136..9155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9815..10606,10607..10675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10438..10457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10676..10780)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga taagcttggg
     2521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2821 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2881 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2941 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     3001 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3061 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag aggatccacc
     3121 ggtgccacca tgggagacat gggagatcca ccaaaaaaaa aacgtctgat ttccctatgt
     3181 gttggttgcg gcaatcagat tcacgatcag tatattctga gggtttctcc ggatttggaa
     3241 tggcatgcgg catgtttgaa atgtgcggag tgtaatcagt atttggacga gagctgtaca
     3301 tgctttgtta gggatgggaa aacctactgt aaaagagatt atatcaggtt gtacgggatc
     3361 aaatgcgcca agtgcagcat cggcttcagc aagaacgact tcgtgatgcg tgcccgctcc
     3421 aaggtgtatc acatcgagtg tttccgctgt gtggcctgca gccgccagct catccctggg
     3481 gacgaatttg cgcttcggga ggacggtctc ttctgccgag cagaccacga tgtggtggag
     3541 agggccagtc taggcgctgg cgacccgctc agtcccctgc atccagcgcg gccactgcaa
     3601 atggcagcgg agcccatctc cgccaggcag ccggccctgc ggccccacgt ccacaagcag
     3661 ccggagaaga ccacccgcgt gcggactgtg ctgaacgaga agcagctgca caccttgcgg
     3721 acctgctacg ccgcaaaccc gcggccagat gcgctcatga aggagcaact ggtagagatg
     3781 acgggcctca gtccccgtgt gatccgggtc tggtttcaaa acaagcggtg caaggacaag
     3841 aagcgaagca tcatgatgaa gcaactccag cagcagcagc ccaatgacaa aactaatatc
     3901 caggggatga caggaactcc catggtggct gccagtccag agagacacga cggtggctta
     3961 caggctaacc cagtggaagt acaaagttac cagccacctt ggaaagtact gagcgacttc
     4021 gccttgcaga gtgacataga tcagcctgct tttcagcaac tggtcaattt ttcagaagga
     4081 ggaccgggct ctaattccac tggcagtgaa gtagcatcaa tgtcctctca acttccagat
     4141 acacctaaca gcatggtagc cagtcctatt gaggcaaggt acaagtccgg actcagatcc
     4201 tacgagggga gaggaagtct tctgacctgc ggagacgtcg aagagaatcc tggacccgct
     4261 agagccacca tggaggcgcg cggggagctg ggcccggccc gggagtcggc gggaggcgac
     4321 ctgctgctag cactgctggc gcggagggcg gacctgcgcc gagagatccc gctgtgcgct
     4381 ggctgtgacc agcacatcct ggaccgcttc atcctcaagg ctctggaccg ccactggcac
     4441 agcaagtgtc tcaagtgcag cgactgccac acgccactgg ccgagcgctg cttcagccga
     4501 ggggagagcg tttactgcaa ggacgacttt ttcaagcgct tcgggaccaa gtgcgccgcg
     4561 tgccagctgg gcatcccgcc cacgcaggtg gtgcgccgcg cccaggactt cgtgtaccac
     4621 ctgcactgct ttgcctgcgt cgtgtgcaag cggcagctgg ccacgggcga cgagttctac
     4681 ctcatggagg acagccggct cgtgtgcaag gcggactacg aaaccgccaa gcagcgagag
     4741 gccgaggcca cggccaagcg gccgcgcacg accatcaccg ccaagcagct ggagacgctg
     4801 aagagcgctt acaacacctc gcccaagccg gcgcgccacg tgcgcgagca gctctcgtcc
     4861 gagacgggcc tggacatgcg cgtggtgcag gtttggttcc agaaccgccg ggccaaggag
     4921 aagaggctga agaaggacgc cggccggcag cgctgggggc agtatttccg caacatgaag
     4981 cgctcccgcg gcggctccaa gtcggacaag gacagcgttc aggaggggca ggacagcgac
     5041 gctgaggtct ccttccccga tgagccttcc ttggcggaaa tgggcccggc caatggcctc
     5101 tacgggagct tgggggaacc cacccaggcc ttgggccggc cctcgggagc cctgggcaac
     5161 ttctccctgg agcatggagg cctggcaggc ccagagcagt accgagagct gcgtcccggc
     5221 agcccctacg gtgtcccccc atcccccgcc gccccgcaga gcctccctgg cccccagccc
     5281 ctcctctcca gcctggtgta cccagacacc agcttgggcc ttgtgccctc gggagccccc
     5341 ggcgggcccc cacccatgag ggtgctggca gggaacggac ccagttctga cctatccacg
     5401 gggagcagcg ggggttaccc cgacttccct gccagccccg cctcctggct ggatgaggta
     5461 gaccacgctc agttctaact cgagggatca agcttatcga aacctggaaa aacatggagc
     5521 aatcacaagt agcaacacag cagctaccaa tgctgcttgt gcctggctag aagcacaaga
     5581 ggaggaggag gtgggttttc cagtcacacc tcagacaatc aacctctgga ttacaaaatt
     5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6001 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6181 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     6241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6301 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6361 acttccctga ttgacagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     6421 gatggtgcta caagctagta ccagttgagc cagataagat agaagaggcc aataaaggag
     6481 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6541 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     6601 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6781 aaatctctag cagggccgct ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6841 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6901 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6961 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7021 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7081 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     7261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7441 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     7501 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7561 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7861 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     7921 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     7981 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     8041 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     8101 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     8161 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     8221 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     8281 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     8341 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     8401 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8461 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8581 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8641 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8821 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9241 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9481 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10801 cacctgacgt c
//
