LOCUS       sequence-174812-        6305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Rentsch D, Laloi M, Rouhara I, Schmelzer E, Delrot S, Frommer WB
  TITLE     NTR1 encodes a high affinity oligopeptide transporter in 
            Arabidopsis.
  JOURNAL   FEBS Lett. 1995 Aug 21;370(3):264-8.
  PUBMED    7656990
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "1995-08-21"; volume: "370(3)"; pages: "264-8"
COMMENT     Sequence Label: pDR195
FEATURES             Location/Qualifiers
     source          1..6305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      151..338
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(206..230)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(350..367)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(350..366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..381)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      802..2148
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(2259..3062)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3063..3278)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3303..3321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3389..3493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3494..3562,3563..4354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3712..3731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4525..5113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5014..5033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5267..5284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5401..5422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5437..5442,5443..5460,5461..5467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5475..5491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5480..5502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5499..5515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5499..5515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcggccgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
       61 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
      121 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
      181 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      241 aaacgaraat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      301 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca ctggccgtcg
      361 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      421 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      481 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      541 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat
      601 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
      661 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
      721 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
      781 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
      841 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
      901 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
      961 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     1021 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     1081 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     1141 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     1201 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     1261 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     1321 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     1381 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     1441 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     1501 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     1561 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     1621 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     1681 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     1741 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact
     1801 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     1861 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     1921 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     1981 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     2041 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     2101 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata
     2161 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt
     2221 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     2281 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     2341 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     2401 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     2461 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     2521 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     2581 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     2641 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     2701 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     2761 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     2821 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     2881 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     2941 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     3001 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     3061 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg
     3121 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     3181 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     3241 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa
     3301 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3661 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3721 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3781 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3841 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3901 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3961 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4021 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4081 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4141 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4201 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4261 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4321 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4381 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4441 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5161 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5221 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5281 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5341 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5401 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5461 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5521 ttacgccaag cttcctgaaa cggagaaaca taaacaggca ttgctgggat cacccataca
     5581 tcactctgtt ttgcctgacc ttttccggta atttgaaaac aaacccggtc tcgaagcgga
     5641 gatccggcga taattaccgc agaaataaac ccatacacga gacgtagaac cagccgcaca
     5701 tggccggaga aactcctgcg agaatttcgt aaactcgcgc gcattgcatc tgtatttcct
     5761 aatgcggcac ttccaggcct cgatcgagac cgtttatcca ttgctttttt gttgtctttt
     5821 tccctcgttc acagaaagtc tgaagaagct atagtagaac tatgagcttt ttttgtttct
     5881 gttttccttt tttttttttt tacctctgtg gaaattgtta ctctcacact ctttagttcg
     5941 tttgtttgtt ttgtttattc caattatgac cggtgacgaa acgtggtcga tggtgggtac
     6001 cgcttatgct cccctccatt agtttcgatt atataaaaag gccaaatatt gtattatttt
     6061 caaatgtcct atcattatcg tctaacatct aatttctctt aaattttttc tctttctttc
     6121 ctataacacc aatagtgaaa atcttttttt cttctatatc tacaaaaact ttttttttct
     6181 atcaacctcg ttgataaatt ttttctttaa caatcgttaa taattaatta attggaaaat
     6241 aaccattttt tctctctttt atacacacat tcaaaagaaa gaaaaaaaat ataccccagc
     6301 ctcga
//
