LOCUS       sequence-174816-        5702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in 
            GABAergic motor neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2842
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             444..761
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
     protein_bind    complement(785..809)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(835..851)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(835..851)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(888..908)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(888..906)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(927..943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(927..943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(940..962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(975..981,982..999,1000..1005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1020..1041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1158..1175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1329..1917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1409..1428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2088..2879,2880..2948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2711..2730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2949..3053)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3079..3534)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3216..3237)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3428..3447
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3661..3683
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3675..3692
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3676..3692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3702..3721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3702..3720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    join(5695..5702,1..17)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtacaaaaaa gcaggctccg aattcgccct tcggatccag atctatgcac gggctccagg
       61 atgacccgga ccttcaggcc cttctgaagg gcagccagct tctgaaggtg aagtccagct
      121 cgtggcgtag ggaacgcttc tacaagctac aggaggactg caagaccatc tggcaggaat
      181 ctcgaaaggt catgaggtcc ccggagtcgc agctgttctc catcgaggac attcaggagg
      241 tacggatggg acaccgcaca gaaggcctgg agaagtttgc ccgagacatc cccgaggatc
      301 gatgcttctc cattgtcttc aaggaccagc gcaacaccct agacctcatt gccccatcac
      361 cagctgacgc tcagcactgg gtgcagggcc tgcgcaagat catccaccac tccggctcca
      421 tggaccagcg gcagaagact agtgagaaaa gtttcgtgat aactgatcca cggctgccag
      481 acaatcccat catcttcgca tccgatggct tcctggagct gaccgagtat tccagagagg
      541 agatcctggg ccgcaatggc cgctttctgc agggaccaga gacagaccag gccacagtgc
      601 agaagattcg cgatgccatt agagatcagc gcgagattac cgtgcagctg ataaactaca
      661 caaaaagcgg gaagaaattc tggaacctcc tgcacctcca gcccatgagg gaccagaagg
      721 gtgagctcca gtatttcatc ggagtgcagc tggatggatg actcgagtaa caagggcgaa
      781 ttcgacccag ctttcttgta caaagtggtg atatcgaatt cctgcagccc gggggatcca
      841 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
      901 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3061 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3121 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3181 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3241 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3301 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3361 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     3541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     3661 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     3721 gcgaattggg taccatcatc atgcccgctg ctgttatcag atggatttga aggagttgtt
     3781 gttaagaaat gagaaataag aaataattgt ataatttttt tttccaaaaa tttaaatttc
     3841 taaactaaaa ttcgaatttt tgcatgcaaa aaacacccac tttttgatct caaatttgca
     3901 cttattaagc actaaaaacc aataatttct tccctttaaa atctaaaaaa aatcttccaa
     3961 taaccccccc actgataaca acaacaaggc atttttcact cgacgaccgg tggcggagcg
     4021 cacgtcttct tattcttctt cttcttcttc ttttcctacc ttttctatcc cactttttcc
     4081 ccggaatttt gagatttgac atgatttttc agagaatttc gagttttgag aaaaaaaaaa
     4141 atcaaaaaag cgattttttg gtcaaaagcc gaaatttaaa gctagttttt ttggaaatat
     4201 cgattttttt tttgagaatt tttcgatttt ccaaaaaaaa aaatcgaaag ttgtttcccg
     4261 aaaagccgaa attttttttg agatatcgaa aaatcggaga atttttcgat gtttcccaga
     4321 aatactaaaa ttttgaattt tccggaaaaa aaatcgttaa aaaattagtt tcttttaaag
     4381 accgaaattt aaagtttttt ttttggaaat atcgattttt tccgagatat ctaaaattga
     4441 aagaaatttt cgattttctg agaaaaatac gaaaaaatcg aaaaaaaaac ccaaatttcg
     4501 gttttttcgt aaaaaaagtc gtaaaaatgt aatttttttc ctgaaaaatc gggatttttt
     4561 acaaaatatc ggaaaatact caaaaaaaag ctgaaaattt cgattttccg gagaaaaaat
     4621 ctttttaaaa aaatattttt tttttcagaa aataagaaaa gccgaaattt ataactattt
     4681 ctccggaaat tcgaaatttt taacgaaata tcggaataat ttttagattt ttcaagtttg
     4741 actttgcgag aaaaaaatcg gaaaaacctc gattcgacgc cgaaaaatgc tccttttcga
     4801 aaagattttt gaaatttcag aaaatcgaca tgcaagcgcg ctccacggcg aaatgacaac
     4861 gatgatccac cgccctcaaa aagttgggtc tcgttaggta tttggcggta aaactggtaa
     4921 aactccagtt ttgcctccaa cgagacccaa ttttttgggg cggtggtgga gcgcgcttgc
     4981 acaagctgaa agcatttttc tgcgactcga taatattttg aaaacctgtg tcaattctcg
     5041 aaattctttt tttaaaaaat aatcccgagc ttctctcagt cctcctctat gaggatgttc
     5101 cttttttttg gtttttcaat tttttttaaa attccaaatt tctgttgtgc aattcacttc
     5161 cccccaagaa atcccaaaaa tccccagttt tccccaaaaa tgttccgttt tcatgtgatt
     5221 tttcccccat ttttaaaaca tttttttgac ttttttttaa aatgattatt attattgttt
     5281 tctatttcat ggccggtaaa ttattttttt tctttctttt tttttgctct tttttttcaa
     5341 gaattttcga attgtttgaa gggctgctca tctaatcttt tgtcattttg ttctgatgcc
     5401 atcatttctg agaggacctt tgaagactcg tcacgaaacg ggaggggggc tcaagtgagc
     5461 attattatta ttattattgt cgcaaaaagt ttaccccggg ctccccctgg ctcccctctt
     5521 tgagcaaggg tttaagggct cattttgatg acgaattgct cattgggatt atagtcacgc
     5581 ccctcttttg gagcaactac acaactgagc cacagtaatc cttgggggcg gggtcagtag
     5641 gaccccctcc ggaataggga aaagctcagt tcaccgccaa aaaagcttga tatcacaagt
     5701 tt
//
