LOCUS       sequence-174817-        5067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version 
            of membrane targeted PH-miniSOG (Q103L) in cholinergic motor 
            neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2851
FEATURES             Location/Qualifiers
     source          1..5067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     142..159
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     169..188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        169..187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    836..860
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1619..1643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1669..1685)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1669..1685)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2422..2442)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2422..2440)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2461..2477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2461..2477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2474..2496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2485..2501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2509..2515,2516..2533,2534..2539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2554..2575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2692..2709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2943..2962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3622..4413,4414..4482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4245..4264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4483..4587)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(4613..5067,1))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4750..4771)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4962..4981
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
       61 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
      181 ctatagggcg aattgggtac cgcttttggt tttcacaatt ttctggtttt tgggattttt
      241 gcgggaattt tgagaggaga aagttgggag agaagatatt ggaatggtcc ccggggagct
      301 gtacatttat acaccaaggc aatcttcgta aaaaataaca atagagtcat ccctcagaac
      361 agaaaagaaa cccgcccggg ggctcaaata cacatgtcat ttttcttttt tcgcctgatt
      421 tttagcctaa attagtgttt agcaattttt taaggtaact cggtggagtc atttgaactt
      481 tttaaactaa aatttcagaa aaaattcttt ttttttgtca aaaaataatt ttcccgccgg
      541 aatttttcaa aaaaataatt tttattttat ttttcaagtt ccttgtttcc tctttatttt
      601 tttattttgc ccccaaaagt tttacactaa aatatttaat tttcaggtaa ttcatcgaaa
      661 gtctttctat tttccgcatc tcttgttcaa aagcttgcat gcctgcaggt cgactctaga
      721 ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
      781 agaacatttt caggaggacc cttggagggt accggtagaa aaaaagcttg atatcacaag
      841 tttgtacaaa aaagcaggct ccgaattcgc ccttcggatc cagatctatg cacgggctcc
      901 aggatgaccc ggaccttcag gcccttctga agggcagcca gcttctgaag gtgaagtcca
      961 gctcgtggcg tagggaacgc ttctacaagc tacaggagga ctgcaagacc atctggcagg
     1021 aatctcgaaa ggtcatgagg tccccggagt cgcagctgtt ctccatcgag gacattcagg
     1081 aggtacggat gggacaccgc acagaaggcc tggagaagtt tgcccgagac atccccgagg
     1141 atcgatgctt ctccattgtc ttcaaggacc agcgcaacac cctagacctc attgccccat
     1201 caccagctga cgctcagcac tgggtgcagg gcctgcgcaa gatcatccac cactccggct
     1261 ccatggacca gcggcagaag actagtgaga aaagtttcgt gataactgat ccacggctgc
     1321 cagacaatcc catcatcttc gcatccgatg gcttcctgga gctgaccgag tattccagag
     1381 aggagatcct gggccgcaat ggccgctttc tgcagggacc agagacagac caggccacag
     1441 tgcagaagat tcgcgatgcc attagagatc agcgcgagat taccgtgcag ctgataaact
     1501 acacaaaaag cgggaagaaa ttctggaacc tcctgcacct ccagcccatg agggaccaga
     1561 agggtgagct ccagtatttc atcggagtgc tgctggatgg atgacaaggg cgaattcgac
     1621 ccagctttct tgtacaaagt ggtgatatcg aattcctgca gcccggggga tccactagtt
     1681 ctagagcggc cgccaccgcg gccgctgtca tcagatcgcc atctcgcgcc cgtgcctctg
     1741 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     1801 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     1861 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     1921 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     1981 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     2041 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     2101 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     2161 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     2221 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     2281 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     2341 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     2401 gccgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2461 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2521 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2581 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2641 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2701 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2761 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3601 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3661 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3721 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3781 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3841 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3901 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3961 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4021 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4081 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4141 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4201 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4261 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4321 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4381 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4441 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     4621 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     4681 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     4741 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4801 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4861 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4921 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4981 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     5041 gcgcttaatg cgccgctaca gggcgcg
//
