LOCUS       sequence-174851-        9873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for testing enhancer fragments in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Sharp B, Paquet E, Naef F, Bafna A, Wijnen H
  TITLE     A new promoter element associated with daily time keeping in 
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2017 Apr 12. doi: 10.1093/nar/gkx268.
  PUBMED    28407113
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Apr 12. doi: 10.1093/nar/gkx268."
COMMENT     Sequence Label: pPTluc
FEATURES             Location/Qualifiers
     source          1..9873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..1882
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(296..314)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1923..2044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1934..1953)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1988..2007
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2160..2361,2362..2392)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2930..2949)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3049..3071
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3109..3127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3195..3299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3300..3368,3369..4160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3518..3537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4331..4919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4820..4839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(5154..5184,5185..5739)
                     /label=P element 5' end
     CDS             complement(join(6518..7132,7203..7334,7538..7853,7915..8569,
                     8644..8917,9319..9390))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9842..9860
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 aggccttcta gaccgcggcc gcagatctgt taacgaattc ggtaccagat ccgggatccg
       61 gcgaaaagag cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
      121 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
      181 acaagctaaa caatcagctt ggcattccgg tactgttggt aaagccacca tggaagacgc
      241 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
      301 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      361 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
      421 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
      481 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
      541 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
      601 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
      661 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
      721 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
      781 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
      841 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
      901 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
      961 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     1021 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
     1081 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     1141 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     1201 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     1261 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     1321 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     1381 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     1441 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     1501 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     1561 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     1621 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     1681 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     1741 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     1801 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     1861 gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat
     1921 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     1981 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2041 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2101 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc gccaagcttt
     2161 gcgtactcgc aaattattaa aaataaaact ttaaaaataa tttcgtctaa ttaatattat
     2221 gagttaattc aaaccccacg gacatgctaa gggttaatca acaatcatat cgctgtctca
     2281 ctcagactca atacgacact cagaatacta ttcctttcac tcgcacttat tgcaagcata
     2341 cgttaagtgg atgtctcttg ccgacgggac caccttatgt tatttcatca tggtctggcc
     2401 attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc
     2461 aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc
     2521 atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa
     2581 aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac
     2641 tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg
     2701 tttatcgcta cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg
     2761 ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc
     2821 acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt
     2881 tccgcgcgat tcggtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     2941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4441 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4921 acgccttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct
     4981 catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg
     5041 gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg
     5101 agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg
     5161 aaataacata aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca
     5221 gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat
     5281 taacccttac gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac
     5341 tggagtaaaa ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag
     5401 ccttgggtgc agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca
     5461 gtggaaggct gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc
     5521 cgtacctagt aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg
     5581 gagtacacaa acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt
     5641 gactgtgcgt taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc
     5701 gtactttgga gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt
     5761 gaaatccaag cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata
     5821 tatataaaca acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa
     5881 cactggatgt cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa
     5941 attaccttaa aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata
     6001 caaatatatt ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga
     6061 cgaaatgaac cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg
     6121 ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa
     6181 gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa
     6241 agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt
     6301 aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg
     6361 atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat
     6421 ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa
     6481 aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa
     6541 gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt
     6601 agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac
     6661 tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt
     6721 ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga
     6781 ggtatactgg caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga
     6841 taaccggcgg acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat
     6901 atccgaagga cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt
     6961 gcagcactcc ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca
     7021 ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat
     7081 aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg
     7141 aatcgattca ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt
     7201 acatttatcg tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct
     7261 ccgttgatat tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc
     7321 aagatggcaa ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta
     7381 aatgaaattt atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa
     7441 gaagggtgtg gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt
     7501 aagcgttata tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt
     7561 ttacgaggag tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact
     7621 gcatgaacca ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca
     7681 gatttttggt ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt
     7741 cgcatatctt ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca
     7801 cctgtacgta aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata
     7861 ggagttgaga tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa
     7921 aagaagtcga cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga
     7981 aggatcttgt caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg
     8041 gtcttgccct tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag
     8101 tccagtccgg aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag
     8161 gcgaatgcca gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg
     8221 atgatcgtgt gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc
     8281 actcgctgcc gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt
     8341 tccctggccg ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc
     8401 tgcatctcct tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact
     8461 tggatgccct gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg
     8521 gaactgccca tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa
     8581 attggtaatt ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact
     8641 caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc
     8701 cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga
     8761 tgtccatatt gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg
     8821 agtcctccgg cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct
     8881 ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa
     8941 tatcaactac aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta
     9001 cgaagtaggt ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg
     9061 gttgttatat tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga
     9121 tcatgatatg atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt
     9181 tcgtgtcact atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac
     9241 gccgcagaca atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta
     9301 tgcacatgta ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg
     9361 aattaatagc tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt
     9421 acccacccaa aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga
     9481 gaggagtttt ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt
     9541 cacgaaaccg ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa
     9601 cggtggcgaa agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag
     9661 agaaggaggc aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga
     9721 aagaacctgg tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt
     9781 tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa
     9841 tgggttttat taacttacat acatactaga att
//
