LOCUS       sequence-174884-       11034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid 
            for integrating, deleting, or replacing a gene in S. cerevisiae. 
            High-copy, URA3 plasmid contains human codon-optimized Cas9 and a 
            guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast 
            chromosome 6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Reider Apel A, d'Espaux L, Wehrs M, Sachs D, Li RA, Tong GJ, Garber 
            M, Nnadi O, Zhuang W, Hillson NJ, Keasling JD, Mukhopadhyay A
  TITLE     A Cas9-based toolkit to program gene expression in Saccharomyces 
            cerevisiae.
  JOURNAL   Nucleic Acids Res. 2017 Jan 9;45(1):496-508. doi: 
            10.1093/nar/gkw1023. Epub 2016 Nov 28.
  PUBMED    27899650
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw1023"; journalName: "Nucleic Acids Res"; date: 
            "2017-01-9- 9"; volume: "45"; issue: "1"; pages: "496-508"
COMMENT     Sequence Label: p426_Cas9_gRNA-R-ARS607c
FEATURES             Location/Qualifiers
     source          1..11034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     558..575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    692..713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(728..733,734..751,752..758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    766..782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     771..793
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     790..806
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     790..806
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     825..845
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        1000..1075
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1161..1865
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1875..5978
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKNERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIYLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5991..6011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6027..6274
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6292..6311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6293..6311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6321..6338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6321..6337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6330..6352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6478..6933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6565..6584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6775..6796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7064..7867)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7868..8083)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8107..8126)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8226..8248
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8286..8304)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      8345..9687
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8677..8724
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9714..9818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9819..9887,9888..10679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10037..10056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10850..11034,1..404)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
       61 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      601 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      661 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      721 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      781 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggag ctagtatact ctttcttcaa caattaaata ctctcggtag
      901 ccaagttggt ttaaggcgca agactgtaat ttatcactac gaaatcttga gatcgggcgt
      961 tcgactcgcc cccgggagac tatttttgct ttctgcacag ttttagagct agaaatagca
     1021 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     1081 ttattttttg tcactattgt tatgtaaaat gccacctctg acagtatgga acgcaaactt
     1141 ctgtctagtg gatagtcgac atccttttgt tgtttccggg tgtacaatat ggacttcctc
     1201 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
     1261 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
     1321 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
     1381 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     1441 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     1501 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga
     1561 aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta
     1621 tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca
     1681 acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt
     1741 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     1801 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     1861 caacttggaa aaaaatggac aagaagtact ccattgggct cgatatcggc acaaacagcg
     1921 tcggttgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
     1981 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
     2041 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
     2101 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
     2161 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag aacgagcgcc
     2221 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
     2281 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
     2341 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
     2401 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
     2461 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
     2521 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
     2581 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
     2641 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
     2701 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
     2761 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
     2821 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
     2881 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
     2941 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
     3001 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
     3061 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
     3121 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
     3181 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
     3241 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
     3301 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
     3361 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
     3421 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
     3481 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
     3541 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
     3601 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
     3661 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
     3721 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
     3781 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
     3841 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
     3901 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
     3961 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
     4021 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
     4081 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
     4141 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
     4201 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
     4261 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
     4321 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
     4381 acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
     4441 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
     4501 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
     4561 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
     4621 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
     4681 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
     4741 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
     4801 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
     4861 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
     4921 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
     4981 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
     5041 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
     5101 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
     5161 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
     5221 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
     5281 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
     5341 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
     5401 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
     5461 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
     5521 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
     5581 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
     5641 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
     5701 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
     5761 agcacaggga taagcccatc agggagcagg cagaaaacat tatctacttg tttactctga
     5821 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
     5881 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
     5941 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
     6001 agaggaaggt gtgatctctt ctcgagtcat gtaattagtt atgtcacgct tacgttcacg
     6061 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     6121 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     6181 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     6241 gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc gccctatagt
     6301 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6361 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6421 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     6481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6901 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     6961 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa
     7021 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     7081 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     7141 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     7201 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     7261 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     7321 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     7381 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     7441 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     7501 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     7561 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     7621 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     7681 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     7741 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     7801 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     7861 tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata
     7921 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     7981 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     8041 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac
     8101 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8161 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8221 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8281 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8341 gtatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga
     8401 gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg
     8461 gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat
     8521 ataagtacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta tatatatata
     8581 caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt
     8641 tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta
     8701 ttctctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg
     8761 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac
     8821 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct
     8881 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat
     8941 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca
     9001 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac
     9061 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt
     9121 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat
     9181 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg
     9241 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct
     9301 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa
     9361 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag
     9421 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa
     9481 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa
     9541 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt
     9601 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc
     9661 tacaaaatga agcacagatg cttcgttcag gtggcacttt tcggggaaat gtgcgcggaa
     9721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aaga
//
