LOCUS       sequence-174886-        7701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BPNLS-Cas9-BPNLS expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, 
            Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, 
            Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X,
            Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, 
            Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang 
            K, Callaway EM, Zhang K, Belmonte JC
  TITLE     In vivo genome editing via CRISPR/Cas9 mediated homology-independent
            targeted integration.
  JOURNAL   Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub
            2016 Nov 16.
  PUBMED    27851729
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20565"; journalName: "Nature"; date: "2016-12-1- 1"; 
            volume: "540"; issue: "7631"; pages: "144-149"
COMMENT     Sequence Label: pCAG-1BPNLS-Cas9-1BPNLS
FEATURES             Location/Qualifiers
     source          1..7701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     229..251
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     313..332
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             426..446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      480..489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             486..4589
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             486..4589
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4635..4655
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4662..4727
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4752..4771)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4817..4872
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4871..4890)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5278..5295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5449..6037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5529..5548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6208..6999,7000..7068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6831..6850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7069..7173)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7204..7583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
       61 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
      121 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
      181 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
      241 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
      301 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattct
      361 aatacgactc actataggga gacgccacca tgaagcggac tgctgatggc agtgaatttg
      421 agtccccaaa gaagaagaga aaggtggaag gtatccacgg agtcccagca gccgaattcg
      481 ccaccatgga caagaagtat tctatcggac tggacatcgg gactaatagc gtcgggtggg
      541 ccgtgatcac tgacgagtac aaggtgccct ctaagaagtt caaggtgctc gggaacaccg
      601 accggcattc catcaagaaa aatctgatcg gagctctcct ctttgattca ggggagaccg
      661 ctgaagcaac ccgcctcaag cggactgcta gacggcggta caccaggagg aagaaccgga
      721 tttgttacct tcaagagata ttctccaacg aaatggcaaa ggtcgacgac agcttcttcc
      781 ataggctgga agaatcattc ctcgtggaag aggataagaa gcatgaacgg catcccatct
      841 tcggtaatat cgtcgacgag gtggcctatc acgagaaata cccaaccatc taccatcttc
      901 gcaaaaagct ggtggactca accgacaagg cagacctccg gcttatctac ctggccctgg
      961 cccacatgat caagttcaga ggccacttcc tgatcgaggg cgacctcaat cctgacaata
     1021 gcgatgtgga taaactgttc atccagctgg tgcagactta caaccagctc tttgaagaga
     1081 accccatcaa tgcaagcgga gtcgatgcca aggccattct gtcagcccgg ctgtcaaaga
     1141 gccgcagact tgagaatctt atcgctcagc tgccgggtga aaagaaaaat ggactgttcg
     1201 ggaacctgat tgctctttca cttgggctga ctcccaattt caagtctaat ttcgacctgg
     1261 cagaggatgc caagctgcaa ctgtccaagg acacctatga tgacgatctc gacaacctcc
     1321 tggcccagat cggtgaccaa tacgccgacc ttttccttgc tgctaagaat ctttctgacg
     1381 ccatcctgct gtctgacatt ctccgcgtga acactgaaat caccaaggcc cctctttcag
     1441 cttcaatgat taagcggtat gatgagcacc accaggacct gaccctgctt aaggcactcg
     1501 tccggcagca gcttccggag aagtacaagg aaatcttctt tgaccagtca aagaatggat
     1561 acgccggcta catcgacgga ggtgcctccc aagaggaatt ttataagttt atcaaaccta
     1621 tccttgagaa gatggacggc accgaagagc tcctcgtgaa actgaatcgg gaggatctgc
     1681 tgcggaagca gcgcactttc gacaatggga gcattcccca ccagatccat cttggggagc
     1741 ttcacgccat ccttcggcgc caagaggact tctacccctt tcttaaggac aacagggaga
     1801 agattgagaa aattctcact ttccgcatcc cctactacgt gggacccctc gccagaggaa
     1861 atagccggtt tgcttggatg accagaaagt cagaagaaac tatcactccc tggaacttcg
     1921 aagaggtggt ggacaaggga gccagcgctc agtcattcat cgaacggatg actaacttcg
     1981 ataagaacct ccccaatgag aaggtcctgc cgaaacattc cctgctctac gagtacttta
     2041 ccgtgtacaa cgagctgacc aaggtgaaat atgtcaccga agggatgagg aagcccgcat
     2101 tcctgtcagg cgaacaaaag aaggcaattg tggaccttct gttcaagacc aatagaaagg
     2161 tgaccgtgaa gcagctgaag gaggactatt tcaagaaaat tgaatgcttc gactctgtgg
     2221 agattagcgg ggtcgaagat cggttcaacg caagcctggg tacctaccat gatctgctta
     2281 agatcatcaa ggacaaggat tttctggaca atgaggagaa cgaggacatc cttgaggaca
     2341 ttgtcctgac tctcactctg ttcgaggacc gggaaatgat cgaggagagg cttaagacct
     2401 acgcccatct gttcgacgat aaagtgatga agcaacttaa acggagaaga tataccggat
     2461 ggggacgcct tagccgcaaa ctcatcaacg gaatccggga caaacagagc ggaaagacca
     2521 ttcttgattt ccttaagagc gacggattcg ctaatcgcaa cttcatgcaa cttatccatg
     2581 atgattccct gacctttaag gaggacatcc agaaggccca agtgtctgga caaggtgact
     2641 cactgcacga gcatatcgca aatctggctg gttcacccgc tattaagaag ggtattctcc
     2701 agaccgtgaa agtcgtggac gagctggtca aggtgatggg tcgccataaa ccagagaaca
     2761 ttgtcatcga gatggccagg gaaaaccaga ctacccagaa gggacagaag aacagcaggg
     2821 agcggatgaa aagaattgag gaagggatta aggagctcgg gtcacagatc cttaaagagc
     2881 acccggtgga aaacacccag cttcagaatg agaagctcta tctgtactac cttcaaaatg
     2941 gacgcgatat gtatgtggac caagagcttg atatcaacag gctctcagac tacgacgtgg
     3001 accacatcgt ccctcagagc ttcctcaaag acgactcaat tgacaataag gtgctgactc
     3061 gctcagacaa gaaccgggga aagtcagata acgtgccctc agaggaagtc gtgaaaaaga
     3121 tgaagaacta ttggcgccag cttctgaacg caaagctgat cactcagcgg aagttcgaca
     3181 atctcactaa ggctgagagg ggcggactga gcgaactgga caaagcagga ttcattaaac
     3241 ggcaacttgt ggagactcgg cagattacta aacatgtcgc ccaaatcctt gactcacgca
     3301 tgaataccaa gtacgacgaa aacgacaaac ttatccgcga ggtgaaggtg attaccctga
     3361 agtccaagct ggtcagcgat ttcagaaagg actttcaatt ctacaaagtg cgggagatca
     3421 ataactatca tcatgctcat gacgcatatc tgaatgccgt ggtgggaacc gccctgatca
     3481 agaagtaccc aaagctggaa agcgagttcg tgtacggaga ctacaaggtc tacgacgtgc
     3541 gcaagatgat tgccaaatct gagcaggaga tcggaaaggc caccgcaaag tacttcttct
     3601 acagcaacat catgaatttc ttcaagaccg aaatcaccct tgcaaacggt gagatccgga
     3661 agaggccgct catcgagact aatggggaga ctggcgaaat cgtgtgggac aagggcagag
     3721 atttcgctac cgtgcgcaaa gtgctttcta tgcctcaagt gaacatcgtg aagaaaaccg
     3781 aggtgcaaac cggaggcttt tctaaggaat caatcctccc caagcgcaac tccgacaagc
     3841 tcattgcaag gaagaaggat tgggacccta agaagtacgg cggattcgat tcaccaactg
     3901 tggcttattc tgtcctggtc gtggctaagg tggaaaaagg aaagtctaag aagctcaaga
     3961 gcgtgaagga actgctgggt atcaccatta tggagcgcag ctccttcgag aagaacccaa
     4021 ttgactttct cgaagccaaa ggttacaagg aagtcaagaa ggaccttatc atcaagctcc
     4081 caaagtatag cctgttcgaa ctggagaatg ggcggaagcg gatgctcgcc tccgctggcg
     4141 aacttcagaa gggtaatgag ctggctctcc cctccaagta cgtgaatttc ctctaccttg
     4201 caagccatta cgagaagctg aaggggagcc ccgaggacaa cgagcaaaag caactgtttg
     4261 tggagcagca taagcattat ctggacgaga tcattgagca gatttccgag ttttctaaac
     4321 gcgtcattct cgctgatgcc aacctcgata aagtccttag cgcatacaat aagcacagag
     4381 acaaaccaat tcgggagcag gctgagaata tcatccacct gttcaccctc accaatcttg
     4441 gtgcccctgc cgcattcaag tacttcgaca ccaccatcga ccggaaacgc tatacctcca
     4501 ccaaagaagt gctggacgcc accctcatcc accagagcat caccggactt tacgaaactc
     4561 ggattgacct ctcacagctc ggaggggatg agggagctga taagcgcacc gccgatggtt
     4621 ccgagttcga aagccccaaa aaaaagcgca aggtcgaaga tgactataag gatcacgacg
     4681 gtgattataa agaccacgat atcgactata aagacgacga tgacaaagag ctgtataaac
     4741 tgctgtaaag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     4801 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     4861 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     4921 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     4981 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5041 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5101 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5161 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgaccgatc cgctgcatta
     5221 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5281 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5341 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5401 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5461 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5521 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5581 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5641 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5701 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5761 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5821 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5881 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5941 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6001 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6061 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6121 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6181 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6241 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6301 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6361 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6421 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6481 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6541 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6601 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6661 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6721 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6781 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6841 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6901 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6961 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7021 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7081 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7141 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     7201 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     7261 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     7321 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     7381 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     7441 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7501 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7561 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     7621 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     7681 cgatgggggc gggggggggg g
//
