LOCUS       sequence-174894-        5125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of GFP (Ubiquitin C 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pUB-GFP
FEATURES             Location/Qualifiers
     source          1..5125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..135
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     375..396
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      438..447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(524..1735)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(668..687)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        1808..1912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1913..1981,1982..2773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2131..2150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2944..3532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3686..3703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3771..3905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3795..3814)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3849..3868
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3911..4106)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3925..4060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    4165..4186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4201..4206,4207..4224,4225..4231)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4239..4255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4244..4266
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4263..4279
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4263..4279
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4622..4641
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4640..4695
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4741..4760
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4847..5125,1..435,436..438,439..441))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4850..5125,1..435,436..438,439..441))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4892..4913)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
       61 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      121 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      181 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
      241 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      301 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      361 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      421 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga
      481 ctgcagaatt cgaagcttga gctcgacctg cagcccaagc ttcgtctaac aaaaaagcca
      541 aaaacggcca gaatttagcg gacaatttac tagtctaaca ctgaaaatta catattgacc
      601 caaatgatta catttcaaaa ggtgcctaaa aaacttcaca aaacacactc gccaaccccg
      661 agcgcatagt tcaaaaccgg agcttcagct acttaagaag ataggtacat aaaaccgacc
      721 aaagaaactg acgcctcact tatccctccc ctcaccagag gtccggcgcc tgtcgattca
      781 ggagagccta ccctaggccc gaaccctgcg tcctgcgacg gagaaaagcc taccgcacac
      841 ctaccggcag gtggccccac cctgcattat aagccaacag aacgggtgac gtcacgacac
      901 gacgagggcg cgcgctccca aaggtacggg tgcactgccc aacggcaccg ccataactgc
      961 cgcccccgca acagacgaca aaccgagttc tccagtcagt gacaaacttc acgtcagggt
     1021 ccccagatgg tgccccagcc catctcaccc gaataagagc tttcccgcat tagcgaaggc
     1081 ctcaagacct tgggttcttg ccgcccacca tgccccccac cttgtttcaa cgacctcaca
     1141 gcccgcctca caagcgtctt ccattcaaga ctcgggaaca gccgccattt tgctgcgctc
     1201 cccccaaccc ccagttcagg gcaaccttgc tcgcggaccc agactacagc ccttggcggt
     1261 ctctccacac gcttccgtcc caccgagcgg cccggcggcc acgaaagccc cggccagccc
     1321 agcagcccgc tactcaccaa gtgacgatca cagcgatcca caaacaagaa ccgcgaccca
     1381 aatcccggct gcgacggaac tagctgtgcc acacccggcg cgtccttata taatcatcgg
     1441 cgttcaccgc cccacggaga tccctccgca gaatcgccga gaagggacta cttttcctcg
     1501 cctgttccgc tctctggaaa gaaaaccagt gccctagagt cacccaagtc ccgtcctaaa
     1561 atgtccttct gctgatactg gggttctaag gccgagtctt atgagcagcg ggccgctgtc
     1621 ctgagcgtcc gggcggaagg atcaggacgc tcgctgcgcc cttcgtctga cgtggcagcg
     1681 ctcgccgtga ggaggggggc gcccgcggga ggcgccaaaa cccggcgcgg aggccagatc
     1741 tggagccgac acgggttact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     1801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2521 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2581 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2641 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3721 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg gatccagaca
     3781 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3841 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3901 aagttagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
     3961 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     4021 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     4081 ctatggttgc tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa
     4141 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     4201 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     4261 cacaggaaac agctatgacc atgattacgc caagcttggg ctgcaggtcg agggatctcc
     4321 ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta
     4381 gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata
     4441 taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg
     4501 gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca gccagcatat
     4561 gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     4621 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     4681 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     4741 ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag
     4801 ccaccacctt ctgataggca gcctgcacct gaggagtgcg gccgctttac ttgtacagct
     4861 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     4921 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     4981 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     5041 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     5101 cggccatgat atagacgttg tggct
//
