LOCUS       sequence-174911-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mVirD2 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Bernardinelli G, Hogberg B
  TITLE     Entirely enzymatic nanofabrication of DNA-protein conjugates.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):e160. doi: 10.1093/nar/gkx707.
  PUBMED    28977490
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-10-13"; volume: "45(18):e160. doi"; pages: 
            " 10.1093/nar/gkx707"
COMMENT     Sequence Label: pmVirD2
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(72..86,87..92,93..109,110..116)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    92..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    124..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             159..170
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             180..197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(939..957)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      953..1000
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacat atggctagca ccatggatat caagcttacc
       61 ggtgaattca atcataaaaa atttatttgc tttgtgagcg gataacaatt ataatagatt
      121 caattgtgag cggataacaa tttcacacag atatcattaa agaggagaaa tctagaatgc
      181 atcatcatca tcatcatccc gatcgcgctc aagtaatcat tcgcattgtg ccaggaggtg
      241 gaaccaagac ccttcagcag ataatcaatc agttggagta cctgtcccgt aagggaaagc
      301 tggaactgca gcgttcagcc cggcatctcg atattcccgt tccgccggat caaatccgtg
      361 agcttgccca aagctgggtt acggaggccg ggatttatga cgaaagtcag tcagacgatg
      421 ataggcaaca agacttaaca acacacatta ttgtaagctt ccccgcaggt accgaccaaa
      481 ccgcagctta tgaagccagc cgggaatggg cagccgagat gtttgggtca ggatacgggg
      541 gtggccgcta taactatctg acagcctacc acgtcgaccg cgatcatcca catttacatg
      601 tcgtggtcaa tcgtcgggaa cttctggggc acgggtggct gaaaatatcc aggcgccatc
      661 cccagctgaa ttatgacggc ttacggaaaa agatggcaga gatttcactt cgtcacggca
      721 tagtcctgga tgcgacttcg cgagcagaaa ggggaatagc agagcgacca atcacatatg
      781 ctgaacatcg ccgccttgag cggatgcagc catggtaacg caaaaacccc gcttcggcgg
      841 ggttttttcg cggatccccc gggctcgagg ttaattaagc ggccgcattg atccggctgc
      901 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      961 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     1021 cgg
//
