LOCUS       sequence-174946-       13704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of constitutively active mutant MKK4 with a 
            C-terminal NLS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 13704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pSLIK 3XFLAG-MKK4-EE-NLS hygro
FEATURES             Location/Qualifiers
     source          1..13704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..1061
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             42..1061
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    1527..2115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1580..1600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1998..2009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2118..2134)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2119..2135)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2644..2824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2850..2867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2856..3080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3126..3554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3213..3232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3423..3444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3563..3583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3568..3897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3748..3883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3810..3829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3945..3992
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4011..4385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4515..4636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4552..4571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4606..4625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4685..4701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4685..4701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4698..4720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4709..4725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4733..4739,4740..4757,4758..4763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4778..4799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4916..4933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5087..5675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5167..5186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5846..6637,6638..6706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6469..6488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6707..6811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6886..6905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7077..7456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7458..7656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7674..7854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7901..8026
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8519..8752
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8937..8981
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9279..9396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9453..9477
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9509..9823
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9517..9535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9553..9571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9588..9606
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9624..9642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9660..9678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9695..9713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9731..9749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9796..9820
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9832..9897
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11011..11031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(11070..11094)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        11144..12355
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     12192..12211
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    13225..13687
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13281..13298)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13508..13527
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 atagcttgcc acaacccgta ccaaagatgg atagatccgg aaagcctgaa ctcaccgcga
       61 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
      121 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
      181 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
      241 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
      301 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
      361 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
      421 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
      481 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
      541 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
      601 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
      661 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
      721 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
      781 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
      841 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
      901 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
      961 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     1021 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgaaca
     1081 agagctgatt tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg
     1141 agagaacggc cttacgcttg gtggcacagt tctcgtccac agttcgctaa gctcgctcgg
     1201 ctgggtcgcg ggagggccgg tcgcagtgat tcaggccctt ctggattgtg ttggtcccca
     1261 gggcacgatt gtcatgccca cgcactcggg tgatctgact gatcccgcag attggagatc
     1321 gccgcccgtg cctgccgatt gggtgcagat ctctagatct gtacaagtaa agcggccgcg
     1381 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1441 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg tttagtccct
     1501 cccaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     1561 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1621 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1681 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     1741 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1801 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1861 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1921 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     1981 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2041 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2101 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     2161 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     2221 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     2281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2341 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     2401 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     2461 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     2521 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     2581 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     2641 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2821 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2881 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2941 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3001 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3061 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3121 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3541 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     3841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3901 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     3961 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     4021 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     4081 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     4141 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     4201 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     4261 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     4321 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     4381 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4441 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4501 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4561 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4621 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     4681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6841 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     6901 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     6961 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     7021 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     7081 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     7141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     7201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     7261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     7321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     7381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     7441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     7501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     7561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     7621 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     7681 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7741 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7801 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7861 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7921 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7981 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8041 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8101 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8161 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8221 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8281 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8341 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8401 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8461 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8521 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8581 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8641 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8701 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8761 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8821 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8881 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8941 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9001 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9061 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9121 cgtttcagac ccacctccca accccgaggg gaccggacag gcccgaagga atagaagaag
     9181 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     9241 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     9301 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     9361 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     9421 gatccagttt ggttaattct agacagttat caacaagttt gtacaaaaaa gcaggcttta
     9481 aaggaaccaa ttcagtcgac tggatcccga gtttactccc tatcagtgat agagaacgta
     9541 tgtcgagttt actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga
     9601 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     9661 ccctatcagt gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg
     9721 tcgagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga
     9781 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gccactagat ggactacaaa
     9841 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagact
     9901 agtggatcca tgcagggtaa acgcaaagca ctgaagttga attttgcaaa tccacctttc
     9961 aaatctacag caaggtttac tctgaatccc aatcctacag gagttcaaaa cccacacata
    10021 gagagactga gaacacacag cattgagtca tcaggaaaac tgaagatctc ccctgaacaa
    10081 cactgggatt tcactgcaga ggacttgaaa gaccttggag aaattggacg aggagcttat
    10141 ggttctgtca acaaaatggt ccacaaacca agtgggcaaa taatggcagt taaaagaatt
    10201 cggtcaacag tggatgaaaa agaacaaaaa caacttctta tggatttgga tgtagtaatg
    10261 cggagtagtg attgcccata cattgttcag ttttatggtg cactcttcag agagggtgac
    10321 tgttggatct gtatggaact catgtctacc tcgtttgata agttttacaa atatgtatat
    10381 agtgtattag atgatgttat tccagaagaa attttaggca aaatcacttt agcaactgtg
    10441 aaagcactaa accacttaaa agaaaacttg aaaattattc acagagatat caaaccttcc
    10501 aatattcttc tggacagaag tggaaatatc aagctctgtg acttcggcat cagtggacag
    10561 cttgtagacg agattgccaa ggaaagagat gctggctgta ggccatacat ggcacctgaa
    10621 agaatagacc caagcgcatc acgacaagga tatgatgtcc gctctgatgt ctggagtttg
    10681 gggatcacat tgtatgagtt ggccacaggc cgatttcctt atccaaagtg gaatagtgta
    10741 tttgatcaac taacacaagt cgtgaaagga gatcctccgc agctgagtaa ttctgaggaa
    10801 agggaattct ccccgagttt catcaacttt gtcaacttgt gccttacgaa ggatgaatcc
    10861 aaaaggccaa agtataaaga gcttctgaaa catcccttta ttttgatgta tgaagaacgt
    10921 gccgttgagg tcgcatgcta tgtttgtaaa atcctggatc aaatgccagc tactcccagc
    10981 tctcccatgt atgtcgatgg tggttctggt ccgaagaaaa aacgtaaagt gtagcaattg
    11041 gaagactaat gcgttttcga gatatctaga cccagctttc ttgtacaaag tggttgataa
    11101 catctcgagc cataaagatg gttaattaac ccacccaaga tctggcctcc gcgccgggtt
    11161 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
    11221 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
    11281 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
    11341 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
    11401 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
    11461 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
    11521 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
    11581 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
    11641 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
    11701 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
    11761 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
    11821 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
    11881 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
    11941 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
    12001 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
    12061 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
    12121 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
    12181 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
    12241 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
    12301 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc
    12361 ttggtaccga gctcggatct ccaccccgta ccggtcctgc aggatttaaa tggcgcgccg
    12421 ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gaattcacca
    12481 tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg
    12541 gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc
    12601 tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg
    12661 acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga
    12721 acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc
    12781 tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt
    12841 gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta
    12901 cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac
    12961 ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg
    13021 gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc
    13081 taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc
    13141 cagcggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
    13201 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
    13261 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
    13321 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
    13381 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
    13441 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
    13501 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
    13561 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
    13621 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
    13681 atgataagct taccggtacg cgtg
//
