LOCUS       sequence-174948-       13785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of constitutively active mutant MKK7 with a 
            C-terminal NLS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 13785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pSLIK 3XFLAG-MKK7-EE-NLS hygro
FEATURES             Location/Qualifiers
     source          1..13785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..1050
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             31..1050
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    1516..2104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1569..1589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1987..1998)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2107..2123)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2108..2124)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2633..2813
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2839..2856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2845..3069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3115..3543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3202..3221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3412..3433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3552..3572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3557..3886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3737..3872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3799..3818
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3934..3981
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4000..4374
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4504..4625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4541..4560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4595..4614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4674..4690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4674..4690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4687..4709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4698..4714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4722..4728,4729..4746,4747..4752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4767..4788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4905..4922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5076..5664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5156..5175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5835..6626,6627..6695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6458..6477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6696..6800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6875..6894)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7066..7445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7447..7645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7663..7843
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7890..8015
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8508..8741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8926..8970
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9268..9385
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9442..9466
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9498..9812
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9506..9524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9542..9560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9577..9595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9613..9631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9649..9667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9684..9702
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9720..9738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9785..9809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9821..9886
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10949..10969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(11008..11032)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        11082..12293
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     12130..12149
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(12418..13035,13036..13041,13042..13077,13078..13080,
                     13081..13116,13117..13119,13120..13155,13156..13164)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    13295..13757
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13351..13368)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13578..13597
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 caacccgtac caaagatgga tagatccgga aagcctgaac tcaccgcgac gtctgtcgag
       61 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
      121 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
      181 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
      241 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
      301 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
      361 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
      421 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
      481 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
      541 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
      601 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
      661 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
      721 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
      781 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
      841 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
      901 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
      961 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     1021 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt
     1081 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc
     1141 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg
     1201 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg
     1261 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc
     1321 ctgccgattg ggtgcagatc tctagatctg tacaagtaaa gcggccgcga ctctagatca
     1381 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1441 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt ttagtccctc ccaattcgat
     1501 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1561 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1621 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1681 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     1741 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     1801 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     1861 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     1921 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     1981 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2041 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2101 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     2161 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     2221 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     2281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     2341 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     2401 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     2461 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     2521 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     2581 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     2641 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2701 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2761 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     2821 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2881 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2941 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3001 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3061 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3421 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3541 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3661 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3721 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3781 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3841 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     3901 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     3961 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     4021 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     4081 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     4141 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     4201 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     4261 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     4321 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     4381 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4441 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4501 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4621 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     6841 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6901 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     6961 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7021 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     7081 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7141 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     7201 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     7261 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     7321 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     7381 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     7441 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     7501 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     7561 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     7621 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     7681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7861 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7921 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     7981 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8041 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8101 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8161 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8221 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8281 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8341 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8401 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8461 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8521 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8581 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8641 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8701 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8761 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8821 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8881 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8941 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9001 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9061 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9121 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     9181 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     9241 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     9301 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     9361 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     9421 gttaattcta gacagttatc aacaagtttg tacaaaaaag caggctttaa aggaaccaat
     9481 tcagtcgact ggatcccgag tttactccct atcagtgata gagaacgtat gtcgagttta
     9541 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     9601 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     9661 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     9721 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     9781 gcagagctcg tttagtgaac cgtcagatcg ccactagatg gactacaaag accatgacgg
     9841 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagacta gaggatccat
     9901 gctggggctc ccatcaacct tgttcacacc gcgcagtatg gagagcatcg agattgacca
     9961 gaagctgcag gagatcatga agcagacagg gtacctgact atcgggggcc agcgttatca
    10021 ggcagaaatc aatgacttgg agaacttggg tgagatgggc agtggtacct gtggtcaggt
    10081 gtggaagatg cggttccgga agacaggcca catcattgct gttaagcaaa tgcggcgctc
    10141 tgggaacaag gaagagaata agcgcatttt gatggacctg gatgtagtac tcaagagcca
    10201 tgactgccct tacatcgttc agtgctttgg caccttcatc accaacacag acgtctttat
    10261 tgccatggag ctcatgggca catgtgcaga gaagctgaag aaacgaatgc agggccccat
    10321 tccagagcga atcctgggca agatgactgt ggcgattgtg aaagcactgt actatctgaa
    10381 ggagaagcat ggcgtcatcc atcgcgatgt caaaccctcc aacatcctgc tagatgagcg
    10441 gggccagatc aagctctgtg actttggcat cagtggccgc cttgttgacg aaaaagccaa
    10501 agaacggagt gctggctgtg ctgcctatat ggctcccgag cgcatcgacc ctccagatcc
    10561 caccaagcct gactatgaca tccgagctga tgtgtggagc ctgggcatct cactggtgga
    10621 gctggcaaca ggacagttcc cctataagaa ctgcaagacg gactttgagg tcctcaccaa
    10681 agtcctacag gaagagcccc cactcctgcc tggtcacatg ggcttctcag gggacttcca
    10741 gtcatttgtc aaagactgcc ttactaaaga tcacaggaag agaccaaagt ataataagct
    10801 acttgaacac agcttcatca agcactatga gatactcgag gtggatgtcg cgtcctggtt
    10861 taaggatgtc atggcgaaga ccgagtcccc aaggactagt ggagtcctga gtcagcacca
    10921 tctgcccttc ttcaggggtg gttctggtcc gaagaaaaaa cgtaaagtgt agcaattgga
    10981 agactaatgc gttttcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca
    11041 tctcgagcca taaagatggt taattaaccc acccaagatc tggcctccgc gccgggtttt
    11101 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
    11161 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
    11221 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
    11281 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
    11341 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
    11401 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
    11461 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
    11521 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
    11581 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
    11641 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
    11701 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
    11761 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
    11821 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
    11881 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
    11941 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
    12001 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
    12061 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
    12121 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
    12181 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
    12241 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
    12301 ggtaccgagc tcggatctcc accccgtacc ggtcctgcag gatttaaatg gcgcgccgct
    12361 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga attcaccatg
    12421 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
    12481 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
    12541 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
    12601 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
    12661 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
    12721 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
    12781 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
    12841 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
    12901 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
    12961 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
    13021 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
    13081 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
    13141 gaccttgaca tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaccccc
    13201 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
    13261 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
    13321 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
    13381 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
    13441 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
    13501 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
    13561 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
    13621 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
    13681 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
    13741 gaaaaacacg atgataagct taccggtacg cgtgatagct tgcca
//
