LOCUS       sequence-17495-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J
  TITLE     Molecular interpretation of ERK signal duration by immediate early 
            gene products.
  JOURNAL   Nat Cell Biol. 2002 Aug . 4(8):556-64.
  PUBMED    12134156
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Aug . 4(8):556-64."
COMMENT     Sequence Label: pcDNA3-FLAG-Fos F343A
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             134..157
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgcctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattcacc atggactaca aagacgatga cgacaagttc tcgggtttca acgcggacta
      181 cgaggcgtca tcctcccgct gcagtagcgc ctccccggcc ggggacagcc tttcctacta
      241 ccattcccca gccgactcct tctccagcat gggctcccct gtcaacacac aggacttttg
      301 cgcagatctg tccgtctcta gtgccaactt tatccccacg gtgacagcca tctccaccag
      361 cccagacctg cagtggctgg tgcagcccac tctggtctcc tccgtggccc catcgcagac
      421 cagagcgccc catccttacg gactccccac cccgtcgacc ggggcttacg ccagagcggg
      481 agtggtgaag accatgtcag gcggcagagc gcagagcatc ggcagaaggg gcaaagtaga
      541 gcagctatct cctgaagagg aagagaaacg gagaatccga agggaaagga ataagatggc
      601 tgcagccaag tgccggaatc ggaggaggga gctgacagat acgctccaag cggagacaga
      661 tcaacttgaa gacgagaagt ctgcgttgca gaccgagatt gccaatctac tgaaagagaa
      721 ggaaaaactg gagtttattt tggcagccca ccgacctgcc tgcaagatcc ccaatgacct
      781 gggcttccca gaggagatgt ctgtgacctc cctggacttg actgggggtc tgcctgaggc
      841 taccacccca gagtctgagg aggccttcac cctgcctctt ctcaatgacc ctgagcccaa
      901 gccatccttg gagccggtca agaacattag caacatggag ctga
//
