LOCUS       sequence-174954-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP5 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP5.v2 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      34..622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     523..542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     776..793
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    910..931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(946..951,952..969,970..976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    984..1000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     989..1011
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1008..1024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1008..1024
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1043..1063
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1045..1063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1091..1317
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1318..1498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1545..1670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2163..2396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2581..2625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2914..3008
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2925..2944
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2962..2980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2990..3008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3109..3226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3275..3785
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3704..3722
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3794..4366
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4374..4393
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4421..4439
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4450..5070
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4523..4542)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5105..5678
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5272..5289)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5499..5518
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5698..6297
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5698..5717)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6194..6214
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6425..6658
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6730..6851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6767..6786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6821..6840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6891..7026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7047..7065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7075..7092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7075..7091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7084..7106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7233..7688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7320..7339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7530..7551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7714..7818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7819..7887,7888..8679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8037..8056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
       61 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      661 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      721 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      781 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      841 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      901 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      961 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     1021 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     1081 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     1141 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     1201 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     1261 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     1321 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1381 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1441 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1501 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1561 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1621 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1681 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1741 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1801 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1861 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1921 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1981 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2041 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2101 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2161 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2221 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2281 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2341 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2401 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2461 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2521 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2581 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2641 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2701 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2761 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2821 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2881 tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg
     2941 gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt
     3001 gatagagaca ccggtcaaag gcaaacgacc aattactgca gtaattggtc gtttgccttt
     3061 gatttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     3121 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     3181 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     3241 ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag
     3301 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     3361 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     3421 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     3481 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     3541 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     3601 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     3661 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     3721 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     3781 cccaggggga tctgtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
     3841 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
     3901 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
     3961 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
     4021 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
     4081 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
     4141 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
     4201 gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc
     4261 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     4321 gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     4381 gctggttatt gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg
     4441 cgaattgata tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
     4501 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
     4561 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
     4621 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
     4681 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
     4741 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
     4801 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
     4861 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
     4921 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
     4981 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
     5041 gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcg gctcccggga gttctaggga
     5101 tctgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5161 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5221 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5281 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5341 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5401 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5461 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5521 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5581 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5641 acggggacgt ggttttcctt tgaaaaacac gatgataagg atccaccgga gcttaccatg
     5701 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5761 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5821 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5881 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5941 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6001 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6061 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6121 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6181 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6241 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6301 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
     6361 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6421 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6481 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6541 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6601 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6661 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6721 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6781 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6841 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6901 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6961 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7021 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     7081 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7141 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7201 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     7261 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7321 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7381 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7441 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7501 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7561 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7621 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7681 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7741 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7801 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7861 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7921 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7981 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8041 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8101 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8161 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8221 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8281 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8341 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8401 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8461 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8521 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8581 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8641 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8701 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8761 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagc
//
