LOCUS       sequence-174969-        9982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR
FEATURES             Location/Qualifiers
     source          1..9982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(193..264,659..932,1007..1661,1723..2038,2259..2390,
                     2461..3075)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(3853..4407,4408..4438)
                     /label=P element 5' end
     rep_origin      complement(4673..5261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4753..4772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5432..6223,6224..6292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6055..6074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6293..6397)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6465..6483
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6521..6543)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6643..6662
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(7200..7230,7231..7432)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        7469..7921
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7868..7889
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(9697..9715)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gtcaatgtcc gccttcagtt gcactttgtc agcggtttcg tgacgaagct ccaagcggtt
       61 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
      121 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
      181 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
      241 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
      301 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
      361 aaaaatccca aaccgcaatc gcacaaacaa atagtgacac gaaacagatt attctggtag
      421 ctgtgctcgc tatataagac aatttttaag atcatatcat gatcaagaca tctaaaggca
      481 ttcattttcg actacattct tttttacaaa aaatataaca accagatatt ttaagctgat
      541 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcgttttgt
      601 tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg
      661 tgacagcgga gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta
      721 cggcacgctc ctgccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc
      781 cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg
      841 ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat
      901 accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc
      961 tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc
     1021 tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg
     1081 aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg
     1141 ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag
     1201 gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca ggccatggtg
     1261 cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag
     1321 gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt
     1381 ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggcactaac cgatccgccg
     1441 cttctgatct gcgatgagcc cacctccgga ctggactcat ttaccgccca cagcgtcgtc
     1501 caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg
     1561 tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct
     1621 ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat
     1681 taagggtatc tagcattaca ttacatctca actcctatcc agcgtgggtg cccagtgtcc
     1741 taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga
     1801 gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgctatta gcaaagtagc
     1861 ccgggatatg gagcagttgt tggccaccaa aaatttggag aagccactgg agcagccgga
     1921 gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc
     1981 ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt
     2041 gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta
     2101 gattttagtt agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata
     2161 gtttatagtt ttgcagaaaa taaataaatt tcatttaact cgcgaacatg ttgaagatat
     2221 gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc
     2281 tcatcttttt gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac ggagccatct
     2341 tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcttg
     2401 tttagaatac atttgcatat taataattta ctaactttct aatgaatcga ttcgatttag
     2461 gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt
     2521 gacacatact ttctgggcaa aacgattgcc gaattaccgc tttttctcac agtgccactg
     2581 gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc
     2641 aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata
     2701 tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca
     2761 ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg
     2821 ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg
     2881 gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc
     2941 aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt
     3001 ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc
     3061 cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt tttaccatta
     3121 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     3181 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     3241 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     3301 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     3361 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     3421 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     3481 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     3541 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     3601 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     3661 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     3721 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     3781 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     3841 agaattaatt cggctgctgc tctaaacgac gcatttcgta ctccaaagta cgaatttttt
     3901 ccctcaagct cttattttca ttaaacaatg aacaggacct aacgcacagt cacgttattg
     3961 tttacataaa tgattttttt tactattcaa acttactctg tttgtgtact cccactggta
     4021 tagccttctt ttatcttttc tggttcaggc tctatcactt tactaggtac ggcatctgcg
     4081 ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg cagccttcca ctgcgaatca
     4141 ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc tgcacccaag gctctgctcc
     4201 cacaattttc tcttaatagc acacttcggc acgtgaatta attttactcc agtcacagct
     4261 ttgcagcaaa atttgcaata tttcattttt ttttattcca cgtaagggtt aatgttttca
     4321 aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag caaacgtgca ctgaatttaa
     4381 gtgtatactt cggtaagctt cggctatcga cgggaccacc ttatgttatt tcatcatggg
     4441 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
     4501 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
     4561 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
     4621 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg tttttccata
     4681 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4741 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4801 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4861 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4921 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4981 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5041 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5101 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5161 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5221 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5281 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5341 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5401 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5461 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5521 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5581 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5641 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5701 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5761 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5821 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5881 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5941 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6001 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6061 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6121 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6181 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6241 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6421 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     6481 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6541 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6601 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     6661 tactgagagt gcaccatatg cggtgtgaaa taccgcaccg aatcgcgcgg aactaacgac
     6721 agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg tgggagacag
     6781 cgaaagagca actacgaaac gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt
     6841 tgaaaagtat gtatataaaa aatatatccc ggtgttttat gtagcgataa acgagttttt
     6901 gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag acaaatccaa agtctacttg
     6961 tggggatgtt cgaaggggaa atacttgtat tctataggtc atatcttgtt tttattggca
     7021 caaatataat tacattagct ttttgagggg gcaataaaca gtaaacacga tggtaataat
     7081 ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc ggctactcct tgcgtcgggc
     7141 ccgaagtctt agagccagat atgcgagcac ccggaagctc acgatgagaa tggccagacc
     7201 atgatgaaat aacataaggt ggtcccgtcg gcaagagaca tccacttaac gtatgcttgc
     7261 aataagtgcg agtgaaagga atagtattct gagtgtcgta ttgagtctga gtgagacagc
     7321 gatatgattg ttgattaacc cttagcatgt ccgtggggtt tgaattaact cataatatta
     7381 attagacgaa attattttta aagttttatt tttaataatt tgcgagtacg caaagcttgg
     7441 ctgcatccaa cgcgttggga gctctccgct agaatcccaa aacaaactgg ttattgtggt
     7501 aggtcatttg tttggcagaa agaaaactcg agaaatttct ctggccgtta ttcgttattc
     7561 tctcttttct ttttgggtct ctccctctct gcactaatgc tctctcactc tgtcacacag
     7621 taaacggcat actgctctcg ttggttcgag agagcgcgcc tcgaatgttc gcgaaaagag
     7681 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
     7741 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
     7801 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
     7861 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
     7921 gaatacaaaa tgcgagccct ggtcgtcatc cgtttgtccc gtgtcaccga tgctacgacg
     7981 tccccggagc gtcagctgga gtcgtgccag cagctctgcg cccagcgcgg ctgggatgtc
     8041 gtcggagtgg ctgaggatct ggatgtctcc ggagctgtcg atccgttcga tcgtaagcgc
     8101 cgtccaaacc tggcccgttg gctggctttc gaggagcaac cgttcgatgt gatcgtggct
     8161 taccgtgtcg accgtttgac ccgatcgatt cgtcatctgc agcagctggt ccactgggct
     8221 gaggatcaca agaagttggt cgtctccgct accgaagctc acttcgatac gaccacgcca
     8281 ttcgctgctg tcgtgatcgc tctcatggga acggtggctc agatggaatt ggaggctatc
     8341 aaagagcgta accgtagcgc tgcccatttc aacattcgtg ccggaaagta ccgtggaagc
     8401 ttgccaccgt ggggttactt gccaacgcgt gtggatggag agtggcgtct gttggtcgat
     8461 ccggtgcagc gtgagcgtat tctggaggtc taccaccgtg tcgtggataa ccacgagccg
     8521 ttgcacttgg tggctcacga tttgaaccag cgtggaattc tgagcccgaa ggattacttc
     8581 gctcagttgc agggacgtga gccgcaggga cgtgcttggt cggctaccgc cttgaagcgt
     8641 agcttgatct ccgaggctat gttgggatac gctaccttga acggaaagac cgtgcgtgat
     8701 gatgatggag ctccgttggt gcgtgctgag ccgatcttga cgcgtgagca gttggaggct
     8761 ctgcgcgccg agttggtgaa gaccgatcgt accaagccgg ctgtgagcac cccgagcctg
     8821 ttgctgcgtg tgttgttctg cgctgtgtgc ggagagcccg cttacaagtt cgctggagga
     8881 ggccgtaagc acccgcgtta ccgttgccgt tccatgggat tcccgaagca ctgcggaaac
     8941 ggaaccgtcg ctatggccga gtgggatgct ttctgcgagg agcaggtgct ggatctgttg
     9001 ggagacgctg agcgtctgga gaaggtctgg gtcgctggct ccgatgctgc cgtggagttg
     9061 gctgagttga acgctgagtt ggtggatttg acgagcttga ttggaagccc ggcttaccgc
     9121 gccggcagcc cgcagcgtga ggctctggat gctcgtatcg aggctctggc cgctcgtcag
     9181 gaggagttgg agggactgga ggctcgcccg tccggatggg agtggcgtga gaccggacag
     9241 cgtttcggag attggtggcg tgagcaggat accgctgcta agaacacctg gttgcgtagc
     9301 atgaacgtgc gtctgacctt cgatgtccgt ggaggattga cccgtaccat cgatttcgga
     9361 gatctgcagg agtacgagca gcacttgcgt ctgggcagcg tggtcgagcg tttgcacacc
     9421 ggaatgagct aagactaaag ccaaatagaa aattattcag ttcctggctt aagtttttaa
     9481 aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat gtaaataact aatacataat
     9541 tatgttagtt ttaagttagc aacaaattga ttttagctat attagctact tggttaataa
     9601 atagaatata tttatttaaa gataattgcg tttttattgt cagggagtga gtttgcttaa
     9661 aaactcgttt agatccacta gaaggcctta gtatgtatgt aagttaataa aacccttttt
     9721 tggagaatgt agatttaaaa aaacatattt tttttttatt ttttactgca ctggacatca
     9781 ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga agtaccaggt
     9841 tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc
     9901 ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc
     9961 accgtttgta gcgttaccta gc
//
