LOCUS       sequence-174974-        8425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the set-2.a cDNA isoform in the C. elegans intestine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Han S, Schroeder EA, Silva-Garcia CG, Hebestreit K, Mair WB, Brunet 
            A
  TITLE     Mono-unsaturated fatty acids link H3K4me3 modifiers to C. elegans 
            lifespan.
  JOURNAL   Nature. 2017 Apr 13;544(7649):185-190. doi: 10.1038/nature21686. 
            Epub 2017 Apr 5.
  PUBMED    28379943
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21686"; journalName: "Nature"; date: "2017-04-13- 
            13"; volume: "544"; issue: "7649"; pages: "185-190"
COMMENT     Sequence Label: pSH9
FEATURES             Location/Qualifiers
     source          1..8425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(6819..6847)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7595..7615
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaactccgaa ctatgatgac gaaaaaatgt tttcttgaat tttcatttta ttgtgaaatt
       61 gaaaactctg catagcccgc agtcacttga aagcacattt aagatggaat caaatggtaa
      121 gaaagctcgt gtaatcagga gattttatcc ggggtatgcc gaggaatata tggtgcactc
      181 gcattagctg gcattttttg taattatcgg atttctacct ttttcttaat ctagtttgtt
      241 atcattgtcc atcatttaca accaaaaatt tgcgtcattt acaaccaaaa attgatctga
      301 caattatcat aattcggttt ttattttcag aagaaacgct ccgatgtgat cctatagtga
      361 atatactttt ccatcctctc tgaaataaac aattatgaag aatgcatcaa tttgcattcg
      421 aaataactgc cgtattttgt ctattggtat ggctgcagta ccatttttct tcgcgtcttt
      481 gcgcagcctt cgggcgctac caataaggct aagccggggt aagttatttc agaattctaa
      541 tcaactaatc taagtctcat acatcattgt caagtgacgc atttttttaa tttctatgtt
      601 aactcgactt taatattctt ttctggtttc accagaaaaa tgctggactg aaaaattgaa
      661 tttcccgcga tttgggaaaa accgcagttt aagcttttgg catgaataca gtgaacacag
      721 gaacaaaaaa ttgacttgtt attttgagtt tatattaaat gttaattttg catctcatat
      781 tttcacaaaa cttgtttgct tgaaaaatgt gcaaataaca agtgaacata catcaaggtt
      841 tatgcagatc agcactgatg caactgatag caaaactgat aagggtcaaa atttcagtgg
      901 ccagcacaaa cacatacaca ttcgtagttc gttttgtgtt tcgtgtattt agcatttatt
      961 tttcaattcc ggcaatttgc cgatttgccg gaaattttga tttttggcaa attgccagtt
     1021 tgccgtttgc cgaacatcaa tttgccgaaa ttttttagag ggatctttat aagacggaaa
     1081 cacttaaaat tgtgcttttt tggaattttt ttcaatgttt caatagaatt agcttacatt
     1141 taaaattaga tgtaggaacg tttataggac gcttacaatt ttgccgatta aaatcgaagt
     1201 tctgaacttt tcaaaaaatg tgcaaaattt tttttgacaa atttgacaaa tcggccggtt
     1261 tgtcgattgt ccggaatttt tcagtttcgg cgatttaccg gtttgccgga aaacatcgtt
     1321 tgccacccac acacacataa agagacgaaa caacacacca tcttcaaaaa atttgtgaat
     1381 cttcatttcc acgtggtttg cagggtttcc catccacccc actctatctc tccaagttga
     1441 attttggcaa aattgaccac acaccgatca aaaactattg tgtgtgtgtg tatttgtgtc
     1501 ttcaataatt cctgtctgtg tctcgcacta tctattgact acaagaacgt gatcgcagct
     1561 ctacgaaacc tttactttat tttagaaata ttttttcatg ctattagcaa aaaaaaagtt
     1621 tttttttgtc taagtacact taaaattttc caaaattttt aagatttttc aaaatgtgcg
     1681 atcaaagatc tgacaaaatg ccaaaatttt gaagaggaaa tttagtcggc ttccaaaatt
     1741 ataagtggca gaaattgagc aattgtcact ttttgacagt aaatagaaaa ttttttgaaa
     1801 aattttgaaa cgctttattt tgttacttgg tcattttggc accataggag tagtttttaa
     1861 cattttcccc actggcgcta cttcatttta aacttttcca aagattttaa tttttaattt
     1921 taaaaaattt catataattc gtctgcgtct tacagtttca ggcttcaaaa atctattaca
     1981 tatcttatct ttgaattcag atgtccacac atgatatgaa ccatcatcct ccgcggaaat
     2041 cgcattcaaa gcgagataag cccagttcaa gcaacagcgg cccaaaaatt gaaaatcata
     2101 agtgcaaatg ggcttggcag aaggttttcg aaacaggaaa gtcgtttttg cggcgtgatg
     2161 gttttccaca ggattgcaaa tcgaaagagg attttgagcg aataaaacgc actggcgtga
     2221 gaaaaacaag tgagaatatg cttgaagatc ctagaaagaa ttttgaaagt cttcaacaat
     2281 catcggtata tcaaaccaac tccttccgga atccacgata cttatgtcga gcccatcttc
     2341 gcgtagattc gtattattgt acaattccac caaaacgaga agtttctttg tttaatatgg
     2401 acgacaattg tacagaagtt ttgctccggg attttgcaaa ggattgtgga aaggttgaga
     2461 aagcttacgt gtgtattcat cccgaaacaa agcggcatat gaaaatggca tatgttaagt
     2521 ttgcgaccgt caaggaggct cacaattttt acagcatgta tcatgctcaa aatttacttg
     2581 ccacaaaatg tacacctcgt attgatcctt tcctgtcaat tctcaatgaa gaatacgaag
     2641 ttgcaactaa tggacaagtc ctcccaattc ttcctgacga tcttgcctca atcgatccga
     2701 gtgttcttcg agatcttcga gccaatttcc tgcgagatca gaatgagaaa tatgagctag
     2761 ctatgagaaa tacgtatgaa gatgaaggag gaatgttatc tggtgttata atggatactt
     2821 ctgatcatta tgagcgtgat tatacaatgg atcatgatgt tggaccttct tcaatgaaaa
     2881 tgtctcctat accaccacct ccgatcaaag aagaatcacc tccaccaccg ccacctcctc
     2941 cagtggcttc cgtttcgaat cttgctccag ttccatcagt acagcttccg tattacaaca
     3001 acattcagcc aagttcaagt actatgcaca tgccagagtt tcgaccaact gaacctccac
     3061 catcgtattc ccgtgaggat ccttaccgaa gcacaagtcg aagctcgctt tctcgccaca
     3121 gaaatagatc gagaagtcca tccgatggta tggatcgttc tggacgtagt tccagccgac
     3181 ggactcatcg aaggcctgaa tcaagaaatg gatcgaaaaa tgcaaatggg gacgttgtga
     3241 agtacgaaac ttataagatg gaaaaacgga aaattaagta cgagggagga aataagaaat
     3301 atgagcaagt tcatataaag gaacggactg ctgtgatacg aggaaaaaat cagttggaga
     3361 atgtttcttc cgaatctgca tcgggaagct cttctgtcga tacttatcca gatttttcag
     3421 atgaagaaag aaagaagaag aaacgtccaa aatctcctaa tcgatcgaaa aaagattccc
     3481 gagcttttgg atgggattca acggacgaat ctgacgagga tactcgaaga cgacgatccg
     3541 gaagatctca gaatcgaagc tcggaacgaa aatttcaaac tacttcctcc tcctcaacac
     3601 ggcgtgaact ttcaagcact cacacaaata gtgtcccaaa tttgaaatct catgaaactc
     3661 cgccgccacc accaccaaaa ggtcatccat cagtgcatct tcaaacacca tatcaacacg
     3721 tacaaccgca aatgatccct gctacttatt ataatttgcc cccacagcat atggctccac
     3781 caccgataac cacgagttta ccaccgtttt gcgatttttc tcaaccacca ccaggattta
     3841 cccccacttt caagcccatc acaaatgctc ctttgccaac tccctatcaa gcatccaaca
     3901 ttccacagcc gggattggtt caaatagcag cactatcagc agctccagaa cctttcagtt
     3961 caataccagg accaccacca gggcctgcgc ctattcaaga agatgttggt agagcagagt
     4021 ctcctgagaa accgtctctt tctgagagat tttctgggat ttttggacca acccaacgcg
     4081 aagaacctgc acaagtcgaa gttgaatatg actatcccct caaacactcg gaatctcatg
     4141 atgatcgtca ttcgttagaa gatatggatg tcgaggtttc cagtgatgga gaaactgtat
     4201 ccaatgttga gaaaatcgag tgtatggagg aaaagaaacg acaggatctc gaacgtattg
     4261 caattgctcg aacaccgatt gtgaaaaagt gcaagaagag aatgatggat gagctcagca
     4321 gaaaagtggc tgaagatatt cgccagcaaa ttatgcgaca gtgctttgct gcattggacg
     4381 aaaaacttca tttgaaagct attgctgacg aagaaaaacg gaaaaaggaa cgagaggaga
     4441 aagcaaggca agaagctgaa aaaccgagca atcatttgat tgctgatatg atgactctct
     4501 acaacaatca atcgtttgcc agctcgtcac gtggattcta cagaaaacaa aagccgattc
     4561 caaaaagcca tcccaaacac caagagcacc atcatcatgc gaaggcatca gtttcgaccc
     4621 cagtccattc gtcaagcacg tcaaggaatt catccgtagc tccaacacct caacgtacag
     4681 tttccacgtc gtcatcgtct tcatcggccg caacatcagc tcgagtatca gaagatgaat
     4741 cggattccga cagcacgccg ggtgaagttc aaagacgcaa aacatcagtt ttgagcaatg
     4801 acaagcgacg aagacgtgct tcgtttagtt caacaagtat tcaaagtagt ccagaaaggc
     4861 aaagagatgt ctcatcgagc tctcgaactt cttcatcttc gtctacaagc tcaatgaagc
     4921 aggaagaaac tgctgatgaa aagtcacgaa aacgcaagtt gattatgtca agcgatgagt
     4981 cgtctacaac aggatcaact gcaacatcag ttgtgtcaag taggcaaagt agtttggagc
     5041 ctcaacaaga gaagacggac ggagaaccac cgaaaaagaa atcacagaca gattttattt
     5101 ctgaacgagt ttcgaagatt gaaggtgaag agagaccact accagagcca gtggaaactt
     5161 ctggtccaat aatcggtgat tcttcgtatc taccatataa aatagtacat tgggagaaag
     5221 caggtataat tgaaatgaat cttccggcaa actccatacg agcccacgag tatcatcctt
     5281 tcacaacgga acactgctat ttcggaatag acgatccaag gcaaccgaaa attcaaatat
     5341 ttgatcattc accgtgcaaa tcggagccag gaagtgaacc tctaaaaata acaccagctc
     5401 catggggacc tatagacaat gttgcggaaa ccggaccctt gatttacatg gatgttgtta
     5461 ctgcacccaa aacagtccag aagaagcaga aaccgagaaa acaagttttt gaaaaggatc
     5521 cctacgagta ttatgaacca ccaccaacca agcgcccagc tcctccgcca cgctttaaga
     5581 aaacattcaa accacgatct gaagaggaaa agaagaaaat catcggagac tgcgaagatc
     5641 tgcccgattt ggaagatcaa tggtatttga gggcagcact taatgaaatg caaagtgaag
     5701 ttaaaagtgc tgatgaattg ccctggaaga aaatgttaac cttcaaagaa atgttgagat
     5761 cagaagatcc tctgttaaga ctcaacccga ttcgttcgaa aaaaggactg ccagatgctt
     5821 tctacgagga tgaagaactc gatggagtaa ttccggtagc tgctggatgc tctcgtgctc
     5881 gaccatacga gaagatgaca atgaagcaga aaaggagtct tgtgagacgt cctgataatg
     5941 aatcgcatcc gactgcaatc ttcagtgaaa gagatgaaac cgcgattcgg catcagcatc
     6001 tggccagtaa agatatgaga ttgctccaac gtcgattgct tacgtcactt ggtgatgcta
     6061 acaatgattt cttcaagata aatcagctca aattccgcaa aaagatgatc aagtttgcac
     6121 gttctcgtat tcacggatgg ggtctctacg cgatggagtc gattgcacca gatgagatga
     6181 ttgtggagta tataggacag acgatccgat ctctggtggc cgaagaacga gaaaaggctt
     6241 atgaacgtcg tggaatcgga agttcatatc tgttccgaat tgatctgcat catgtgatcg
     6301 atgcaacaaa gcgtggaaat tttgctcgat ttattaatca ctcgtgccaa cctaattgct
     6361 acgcgaaggt tcttacaatc gaaggcgaga agcggatagt catctacagc cgaacaatta
     6421 tcaagaaggg tgaagaaatc acgtatgact acaagttccc aattgaagac gacaaaatag
     6481 attgtctgtg tggtgcgaag acgtgtcgtg gatatcttaa ttgaggggct gtctcatcct
     6541 actttcacct agttaactgc ttgtcttaaa atctatgctt ctctttagta tctaaaattt
     6601 tcctagaagc ttacaagtat ataaatggtc tcttctcaat aaaggttgta tatttattca
     6661 tcttattgaa tctgccattt cctcgttttt gcgagtttat ataccttcca attttctttc
     6721 tattgtattt tcaacttcta attttaattc agggaaactg cttcaacgca tcggtaccgg
     6781 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     6841 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     6901 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
     6961 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
     7021 tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc
     7081 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     7141 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
     7201 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     7261 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     7321 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
     7381 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa
     7441 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     7501 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     7561 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     7621 ctgggattac acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag
     7681 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     7741 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     7801 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     7861 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     7921 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     7981 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     8041 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     8101 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     8161 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     8221 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     8281 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     8341 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     8401 gtttgggttt gaggctcagt ggaag
//
