LOCUS       sequence-174982-        4089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses 7A mutant BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM, 
            Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo 
            H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE, 
            Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY, 
            Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll 
            JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
  TITLE     Response and resistance to BET bromodomain inhibitors in 
            triple-negative breast cancer.
  JOURNAL   Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub 
            2016 Jan 6.
  PUBMED    26735014
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16508"; journalName: "Nature"; date: "2016-01-21- 
            21"; volume: "529"; issue: "7586"; pages: "413-7"
COMMENT     Sequence Label: pcDNA5-Flag-BRD4-7A
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2212..2229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtctgcgg agagcggccc tgggacgaga ttgagaaatc tgccagtaat gggggatgga
       61 ctagaaactt cccaaatgtc tacaacacag gcccaggccc aaccccagcc agccaacgca
      121 gccagcacca accccccgcc cccagagacc tccaacccta acaagcccaa gaggcagacc
      181 aaccaactgc aatacctgct cagagtggtg ctcaagacac tatggaaaca ccagtttgca
      241 tggcctttcc agcagcctgt ggatgccgtc aagctgaacc tccctgatta ctataagatc
      301 attaaaacgc ctatggatat gggaacaata aagaagcgct tggaaaacaa ctattactgg
      361 aatgctcagg aatgtatcca ggacttcaac actatgttta caaattgtta catctacaac
      421 aagcctggag atgacatagt cttaatggca gaagctctgg aaaagctctt cttgcaaaaa
      481 ataaatgagc tacccacaga agaaaccgag atcatgatag tccaggcaaa aggaagagga
      541 cgtgggagga aagaaacagg gacagcaaaa cctggcgttt ccacggtacc aaacacaact
      601 caagcatcga ctcctccgca gacccagacc cctcagccga atcctcctcc tgtgcaggcc
      661 acgcctcacc ccttccctgc cgtcaccccg gacctcatcg tccagacccc tgtcatgaca
      721 gtggtgcctc cccagccact gcagacgccc ccgccagtgc ccccccagcc acaaccccca
      781 cccgctccag ctccccagcc cgtacagagc cacccaccca tcatcgcggc caccccacag
      841 cctgtgaaga caaagaaggg agtgaagagg aaagcagaca ccaccacccc caccaccatt
      901 gaccccattc acgagccacc ctcgctgccc ccggagccca agaccaccaa gctgggccag
      961 cggcgggaga gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag
     1021 cacccagcac cagagaagag cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc
     1081 ctcaaggaga tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta caagcctgtg
     1141 gacgtggagg cactgggcct acacgactac tgtgacatca tcaagcaccc catggacatg
     1201 agcacaatca agtctaaact ggaggcccgt gagtaccgtg atgctcagga gtttggtgct
     1261 gacgtccgat tgatgttctc caactgctat aagtacaacc ctcctgacca tgaggtggtg
     1321 gccatggccc gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat gccggacgag
     1381 cctgaggagc cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg
     1441 gccccgcccg catccagcga cgccagcagc gatgcctccg cggacagtga cgctgcgact
     1501 gatgacgctg aggaggagcg agcccagcgg ctggctgagc tccaggagca gctcaaagcc
     1561 gtgcacgagc agcttgcagc cctctctcag ccccagcaga acaaaccaaa gaaaaaggag
     1621 aaagacaaga aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt ggaagagaat
     1681 aaaaaaagca aagccaagga acctcctcct aaaaagacga agaaaaataa tagcagcaac
     1741 agcaatgtga gcaagaagga gccagcgccc atgaagagca agccccctcc cacgtatgag
     1801 tcggaggaag aggacaagtg caagcctatg tcctatgagg agaagcggca gctcagcttg
     1861 gacatcaaca agctccccgg cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg
     1921 gagccctccc tgaagaattc caaccccgac gagattgaaa tcgactttga gaccctgaag
     1981 ccgtccacac tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa
     2041 cctcaagctg agaaagttga tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc
     2101 tcagagtcgg agagctccag tgagtccagc tcctctgaca gcgaagactc cgaaacagag
     2161 atggctccga agtcaaaaaa gaaggggcac cccgggaggg agcagaagaa gcaccatcat
     2221 caccaccatc agcagatgca gcaggccccg gctcctgtgc cccagcagcc gcccccgcct
     2281 ccccagcagc ccccaccgcc tccacctccg cagcagcaac agcagccgcc acccccgcct
     2341 cccccaccct ccatgccgca gcaggcagcc ccggcgatga agtcctcgcc cccacccttc
     2401 attgccaccc aggtgcccgt cctggagccc cagctcccag gcagcgtctt tgaccccatc
     2461 ggccacttca cccagcccat cctgcacctg ccgcagcctg agctgccccc tcacctgccc
     2521 cagccgcctg agcacagcac tccaccccat ctcaaccagc acgcagtggt ctctcctcca
     2581 gctttgcaca acgcactacc ccagcagcca tcacggccca gcaaccgagc cgctgccctg
     2641 cctcccaagc ccgcccggcc cccagccgtg tcaccagcct tgacccaaac acccctgctc
     2701 ccacagcccc ccatggccca acccccccaa gtgctgctgg aggatgaaga gccacctgcc
     2761 ccacccctca cctccatgca gatgcagctg tacctgcagc agctgcagaa ggtgcagccc
     2821 cctacgccgc tactcccttc cgtgaaggtg cagtcccagc ccccaccccc cctgccgccc
     2881 ccaccccacc cctctgtgca gcagcagctg cagcagcagc cgccaccacc cccaccaccc
     2941 cagccccagc ctccacccca gcagcagcat cagccccctc cacggcccgt gcacttgcag
     3001 cccatgcagt tttccaccca catccaacag cccccgccac cccagggcca gcagcccccc
     3061 catccgcccc caggccagca gccacccccg ccgcagcctg ccaagcctca gcaagtcatc
     3121 cagcaccacc attcaccccg gcaccacaag tcggacccct actcaaccgg tcacctccgc
     3181 gaagccccct ccccgcttat gatacattcc ccccagatgt cacagttcca gagcctgacc
     3241 caccagtctc caccccagca aaacgtccag cctaagaaac aggagctgcg tgctgcctcc
     3301 gtggtccagc cccagcccct cgtggtggtg aaggaggaga agatccactc acccatcatc
     3361 cgcagcgagc ccttcagccc ctcgctgcgg ccggagcccc ccaagcaccc ggagagcatc
     3421 aaggcccccg tccacctgcc ccagcggccg gaaatgaagc ctgtggatgt cgggaggcct
     3481 gtgatccggc ccccagagca gaacgcaccg ccaccagggg cccctgacaa ggacaaacag
     3541 aaacaggagc cgaagactcc agttgcgccc aaaaaggacc tgaaaatcaa gaacatgggc
     3601 tcctgggcca gcctagtgca gaagcatccg accaccccct cctccacagc caagtcatcc
     3661 agcgacagct tcgagcagtt ccgccgcgcc gctcgggaga aagaggagcg tgagaaggcc
     3721 ctgaaggctc aggccgagca cgctgagaag gagaaggagc ggctgcggca ggagcgcatg
     3781 aggagccgag aggacgagga tgcgctggag caggcccggc gggcccatga ggaggcacgt
     3841 cggcgccagg agcagcagca gcagcagcgc caggagcaac agcagcagca gcaacagcaa
     3901 gcagctgcgg tggctgccgc cgccacccca caggcccaga gctcccagcc ccagtccatg
     3961 ctggaccagc agagggagtt ggcccggaag cgggagcagg agcgaagacg ccgggaagcc
     4021 atggcagcta ccattgacat gaatttccag agtgatctat tgtcaatatt tgaagaaaat
     4081 cttttctga
//
