LOCUS       sequence-174988-        8485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Toxin-antitoxin stabilized bioluminescent reporter plasmid for 
            Streptococcus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pLZ12Km2-P23R:TA:fflucRT
FEATURES             Location/Qualifiers
     source          1..8485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     472..494
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     486..503
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     487..503
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     513..532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        513..531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4660..4676)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4661..4677)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(5499..5510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(7151..7945)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 aagttttggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt ggaataaaga
       61 agcgaaaaag gagaagtcgg ttcagaaaaa gaaggatatg gatctggagc tgtaatataa
      121 aaaccttctt caactaacgg ggcaggttag tgacattaga aaaccgactg taaaaagtac
      181 agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca attcctgaat
      241 agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta cctgtaaaga
      301 tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa taatgggtag
      361 aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag tccatgctgg
      421 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
      481 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
      541 agctctcgaa aagccctgac aacccttgtt cctaaaaagg aataagcgtt cggtcagtaa
      601 ataatagaaa taaaaaatca gacctaagac tgatgacaaa aagagaaaat tttgataaaa
      661 tagtcttaga attaaattaa aaagggaggc caaatataat gaaaaatatg aatgacaatg
      721 atgttccgcg gtggcggcaa ttcggaggat atatatggaa gatgctaaga atatcaagaa
      781 aggtcctgcc ccattttacc cacttgagga tggtactgct ggggaacaat tgcataaggc
      841 tatgaaacgt tacgccctag ttccaggtac tatcgctttt acagatgctc atattgaggt
      901 gaatattaca tatgcggaat attttgaaat gagcgttcgc cttgcggaag ctatgaagcg
      961 ttacggacta aatactaatc atcgaatcgt tgtttgcagt gaaaactctt tgcagttctt
     1021 tatgcctgtc ttaggtgcac tttttattgg agtagcagtt gctcctgcaa atgatattta
     1081 caatgaacga gaattactga atagcatgaa catttctcaa ccaactgtag tgtttgtttc
     1141 taagaaagga ttacagaaaa tcttgaatgt gcagaaaaag ctaccaataa ttcagaagat
     1201 cattattatg gacagcaaga ctgattacca gggtttccaa tcaatgtaca ctttcgtaac
     1261 cagtcatttg ccgcctggct tcaatgagta cgactttgtc ccagaatctt ttgatcgaga
     1321 taagacaatt gcgttaatta tgaatagctc aggttctact ggtctaccta aaggcgtggc
     1381 tttgcctcac cgtgctttat gcgtacgctt ttctcacgct cgtgatccta tttttggaaa
     1441 tcagattgct ccagatacag cgattttatc tgttgtgcct ttccatcatg gctttggaat
     1501 gtttacaact ttgggctacc tcatctgtgg ctttagggtt gtgctaatgt atcgctttga
     1561 ggaagaatta tttctacgat ccttacaaga ctataagata cagacggcac ttttagttcc
     1621 aacgttattt agttttctcg caaaaagtac tctaatcgat aaatatgatt tatctaactt
     1681 gcatgagata gcttctggag gcgcaccatt gtcaaaagaa gtcggtgagg ctgttgcaaa
     1741 aagatttcat ttaccaggca ttcgtcaagg gtacggtcta acggaaacaa catcggctat
     1801 tcttattaca ccaaaaggtg atgataagcc tggggctgtt ggaaaagttg ttcctttctt
     1861 cgaagcaaag gtagtggatc ttgatactgg aaaaacatta ggcgttaacc aaagaggcga
     1921 gctgtgtgtc agaggcccta tgattatgag tggctacgtt aataatccag aggctacgaa
     1981 cgcattaatt gataaagatg gatggttgca ttcaggtgac cttgcttatt gggatgagga
     2041 tgaacacttc tttatagtag gacgactaaa atctctcatc aagtataaag gatatcaagt
     2101 cgccccagct gaattggagt caattctttt acaacatcct aatatctttg acgctggagt
     2161 cgctggtttg cctgacgatg atgcgggtga actcccggct gctgtggtcg tgcttgagca
     2221 tggaaaaacc atgacagaga aagaaattgt ggattacgta gcctcacaag ttactaccgc
     2281 taagaaatta cgtggtggtg tagttttcgt cgatgaagtt cctaaaggat taactggtaa
     2341 acgtgacgct cgtaagattc gtgaaatttt gataaaggcg aaaaagggtg gcaaaagcaa
     2401 actgttaatt aaggatcctc tagaggtacc gtcgacaaaa ggggcccgag cttaagaatt
     2461 gccgctctag agtcgtgtta atttttatga aggcttgaaa aatggaaact tggcttcttc
     2521 tattgttcat ttgactgata atttagactt gatccctggc acgtttgatt tgatgttact
     2581 gccaaaatta actcgttcat ggacgtttga aaatgaaagt agattgcttg ctactctttt
     2641 agcaccttta aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt
     2701 ttatacaaat aatgcaatcg tggcgagtga ttacgttatg atccctttac aagcagaaga
     2761 agaaagtaca aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt
     2821 taaccctgga ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac
     2881 gataaaatca aacctggaag aactgtataa gcaacataaa gaagataact tggttttcca
     2941 aaatattatc aagcgaagta ataaagtaag tacctggtct aaaaacggca ttacagagca
     3001 taaaggctat gacaaaaaag ttttatccat gtataagaac gtattttttg aaatgcttga
     3061 gcgaattatt caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca
     3121 caaatcactt gtgatttgtg attgttgatg ataaaataag agtaagaaga aatagaaaga
     3181 agtgagtgat tgtgggaaat ttaggcgcac aaaaagcaaa acgaaatgat acaccaatca
     3241 gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
     3301 tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gatcaagcct
     3361 tagaagaata tatacggaaa tatttacctg acaaacttta aaaggagtga aaatgaaatg
     3421 gcagttacgt atgaaaaaac atttgaaata gagatcatta acgaattatc ggcaagcgtt
     3481 tataatcgag tattaaacta tgttttgaac catgaattaa ataaaaatga ctctcaatta
     3541 ttggaagtca atttattaaa ccaattaaag cttgcaaaac gtgtaaatct ttttgattat
     3601 tctttagaag aattacaagc cgttcatgag tattggcggt caatgaatcg ttactcaaaa
     3661 caagttttga ataaagagaa agtggcttaa tatggcaaat atagtcaatt ttactgacaa
     3721 acaatttgag aatcgcttaa atgataattt agaagaattg attcaaggaa aaaaagcggt
     3781 tgaatcgcca accgcttttt tacttggtgg gcaaccaggg tcagggaaaa ccagtttgcg
     3841 atcagcaatt tttgaagaaa cacaagggaa tgttattgtc attgataatg atacctttaa
     3901 acaacagcac cctaattttg atgaactagt gaaactttat gaaaaagacg tagtaaaaca
     3961 cgttacccct tattctaatc gcatgacaga agcgatcata agccgtttga gcgatcaagg
     4021 gtataatttg gtgatcgaag gtacaggacg aacaacagac gttcctattc aaaccgcaac
     4081 aatgcttcaa gccaaaggtt atgaaacaaa aatgtatgtc atggcagtac ctaaaatcaa
     4141 ctcatattta ggaacaattg aacgatatga aaccatgtat gcagatgatc caatgacagc
     4201 cagggcaaca ccaaaacaag cgcatgatat tgttgtcaaa aacttaccga ccaatttaga
     4261 aacccttcat aaaacgggct tatttagcga tataaggcta tataacagag aaggagtaaa
     4321 actctattca agtttggaaa caccttctat tagtccaaaa gaaaccttag aaaaagaatt
     4381 gaatcgtaaa gtatcaggga aagaaattca accgacttta gaacgaatag agcaaaaaat
     4441 ggttctaaat aaacaccaag agacacctga atttaaagca attcaacaaa aattggaaag
     4501 cttgcagcca cctacaccac caatacccaa aacacctaaa cttccaggta tttaaacctt
     4561 aaaaaaggaa aagagtagtt accaaaaaac ggtaactact ctttttttat aaaaacattt
     4621 cctcatgttg gacctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg
     4681 ggggcccatc agtctgacga ccaagagagc cataaacacc aatagcctta acatcatccc
     4741 catatttatc caatattcgt tccttaattt catgaacaat cttcattctt tcttctctag
     4801 tcattattat tggtccattc actattctca ttcccttttc agataatttt agatttgctt
     4861 ttctaaataa gaatatttgg agagcaccgt tcttattcag ctattaataa ctcgtcttcc
     4921 taagcatcct tcaatccttt taataacaat tatagcatct aatcttcaac aaactggccc
     4981 gtttgttgaa ctactcttta ataaaataat ttttccgttc ccaattccac attgcaataa
     5041 tagaaaatcc atcttcatcg gctttttcgt catcatctgt atgaatcaaa tcgccttctt
     5101 ctgtgtcatc aaggtttaat tttttatgta tttcttttaa caaaccacca taggagatta
     5161 accttttacg gtgtaaacct tcctccaaat cagacaaacg tttcaaattc ttttcttcat
     5221 catcggtcat aaaatccgta tcctttacag gatattttgc agtttcgtca attgccgatt
     5281 gtatatccga tttatattta tttttcggtc gattttttat taaaacgtct caaaatcgtt
     5341 tctgagacgt tttagcgttt atttcgttta gttatcggca taatcgttaa aacaggcgtt
     5401 atcgtagcgt aaaagccctt gagcgtagcg tggctttgca gcgaagatgt tgtctgttag
     5461 attatgaaag ccgatgactg aatgaaataa taagcgcagc gtccttctat ttcggttgga
     5521 ggaggctcaa gggagtttga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc
     5581 ttgcaatttt gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa
     5641 aaaatggggg gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga
     5701 gtgccaattt ttgtgccaaa aacgctctat cccaactggc tcaagggttt gaggggtttt
     5761 tcaatcgcca acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt
     5821 agctttattg ttgtttttat gattacaaag tgatacacta attttataaa attatttgat
     5881 tggagttttt taaatggtga tttcagaatc gaaaaaaaga gttatgattt ctctgacaaa
     5941 agagcaagat aaaaaattaa cagatatggc gaaacaaaaa ggtttttcaa aatctgcggt
     6001 tgcggcgtta gctatagaag aatatgcaag aaaggaatca gaacaaaaaa aataagcgaa
     6061 agctcgcgtt tttagaagga tacgagtttt cgctacttgt ttttgataag gtaattatat
     6121 catggctatt aaaaatacta aagctagaaa ttttggattt ttattatatc ctgactcaat
     6181 tcctaatgat tggaaagaaa aattagagag tttgggcgta tctatggctg tcagtccttt
     6241 acacgatatg gacgaaaaaa aagataaaga tacatggaat agtagtgatg ttatacgaaa
     6301 tggaaagcac tataaaaaac cacactatca cgttatatat attgcacgaa atcctgtaac
     6361 aatagaaagc gttaggaaca agattaagcg aaaattgggg aatagttcag ttgctcatgt
     6421 tgagatactt gattatatca aaggttcata tgaatatttg actcatgaat caaaggacgc
     6481 tattgctaag aataaacata tatacgacaa aaaagatatt ttgaacatta atgattttga
     6541 tattgaccgc tatataacac ttgatgaaag ccaaaaaaga aaattgaaga atttactttt
     6601 agatatagtg gatgactata atttggtaaa tacaaaagat ttaatggctt ttattcgcct
     6661 taggggagcg gagtttggaa ttttaaatac gaatgatgta aaagatattg tttcaacaaa
     6721 ctctagcgcc tttagattat ggtttgaggg caattatcag tgtggatata gagcaagtta
     6781 tgcaaaggtt cttgatgctg aaacggggga aataaaatga caaacaaaga aaaagagtta
     6841 tttgctgaaa atgaggaatt aaaaaaagaa attaaggact taaaagagcg tattgaaaga
     6901 tacagagaaa tggaagttga attaagtaca acaatagatt tattgagagg agggattatt
     6961 gaataaataa aagcccccct gacgaaagtc gacgatacaa attcctcgta ggcgctcggg
     7021 acccctatct agcgaacttt tagaaaagat ataaaacatc agagtatgga cagttgcgga
     7081 tgtacttcag aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta
     7141 aatctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     7201 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     7261 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     7321 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     7381 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     7441 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     7501 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     7561 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     7621 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     7681 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     7741 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     7801 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     7861 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     7921 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     7981 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     8041 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     8101 agtatcgacg gagccgattt tgaaaccaca attatgatag aatttacaag ctataaggtt
     8161 attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga
     8221 tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc
     8281 ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct
     8341 cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta
     8401 attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa
     8461 atggttcgct gggtttatcg tcgac
//
