LOCUS       sequence-17499-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J
  TITLE     Molecular interpretation of ERK signal duration by immediate early 
            gene products.
  JOURNAL   Nat Cell Biol. 2002 Aug . 4(8):556-64.
  PUBMED    12134156
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Aug . 4(8):556-64."
COMMENT     Sequence Label: pcDNA3-FLAG-Fos DD Y345A
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
      121 accatggact acaaagacga tgacgacaag ttctcgggtt tcaacgcgga ctacgaggcg
      181 tcatcctccc gctgcagtag cgcctccccg gccggggaca gcctttccta ctaccattcc
      241 ccagccgact ccttctccag catgggctcc cctgtcaaca cacaggactt ttgcgcagat
      301 ctgtccgtct ctagtgccaa ctttatcccc acggtgacag ccatctccac cagcccagac
      361 ctgcagtggc tggtgcagcc cactctggtc tcctccgtgg ccccatcgca gaccagagcg
      421 ccccatcctt acggactccc caccccgtcg accggggctt acgccagagc gggagtggtg
      481 aagaccatgt caggcggcag agcgcagagc atcggcagaa ggggcaaagt agagcagcta
      541 tctcctgaag aggaagagaa acggagaatc cgaagggaaa ggaataagat ggctgcagcc
      601 aagtgccgga atcggaggag ggagctgaca gatacgctcc aagcggagac agatcaactt
      661 gaagacgaga agtctgcgtt gcagaccgag attgccaatc tactgaaaga gaaggaaaaa
      721 ctggagttta ttttggcagc ccaccgacct gcctgcaaga tccccaatga cctgggcttc
      781 ccagaggaga tgtctgtgac ctccctggac ttgactgggg gtctgcctga ggctaccacc
      841 ccagagtctg aggaggcctt caccct
//
