LOCUS       sequence-174990-       13409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toxin-antitoxin stabilized bioluminescent reporter plasmid for 
            Streptococcus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 13409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pLZ12Km2-P23R:TA:Xen5
FEATURES             Location/Qualifiers
     source          1..13409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2712..2728)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2713..2729)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(3551..3562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5203..5997)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     7009..7031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7023..7040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7024..7040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7050..7069
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7050..7068
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7294..8376
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             8397..9380
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             9400..10842
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTRNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     NVAVEIEQTRFLEEDKFLVFVP"
     CDS             10862..11785
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             11805..12917
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 ctgctatacg ccgcggggtt tacttttacc ttatggaact tgatatgttc attatcagct
       61 tccatatatc cctctgtcat gccattcttt atcagcccgt accagcattc cgctaaggcg
      121 cgactgttat tcatatctgt gccgaatacg cgaaagtcct tgttgtaaag ccctcggcaa
      181 ataccaaacc gaaatcgtcc ttttgacatt tgatccaata aattcacatc ttcaagttgg
      241 cgtactggat gggctgtggg aagaacaata gcggcagttc ctacattcaa ttttttagtc
      301 gcgccaagta aatatgcagc agcgacataa gggttaccaa gcaaaccaaa ctccgtgaaa
      361 tgatgctcca gtaaccatac ggtatcaaaa ccacactcct cagagatgcg acctaattta
      421 accaaacgtt tcattacctc tgtttgagaa aattggggag gttggtatgt aagcaaaaag
      481 tttccaaatt tcatttttta ttcctccttg cggccgctct agagtcgtgt taatttttat
      541 gaaggcttga aaaatggaaa cttggcttct tctattgttc atttgactga taatttagac
      601 ttgatccctg gcacgtttga tttgatgtta ctgccaaaat taactcgttc atggacgttt
      661 gaaaatgaaa gtagattgct tgctactctt ttagcacctt taaaaagtga ctatgatctc
      721 attattattg atactgtacc aacgccaagc gtttatacaa ataatgcaat cgtggcgagt
      781 gattacgtta tgatcccttt acaagcagaa gaagaaagta caaacaacat tcaaaactat
      841 atttcctatt tgattgattt acaagaacaa tttaaccctg gactagatat gatcggtttt
      901 gttccttatt tagttgatac ggacagcgca acgataaaat caaacctgga agaactgtat
      961 aagcaacata aagaagataa cttggttttc caaaatatta tcaagcgaag taataaagta
     1021 agtacctggt ctaaaaacgg cattacagag cataaaggct atgacaaaaa agttttatcc
     1081 atgtataaga acgtattttt tgaaatgctt gagcgaatta ttcaattaga aaacgaaaaa
     1141 gaatagaatc acaaatcaca agtgattaat cacaaatcac ttgtgatttg tgattgttga
     1201 tgataaaata agagtaagaa gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc
     1261 acaaaaagca aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa
     1321 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg
     1381 cggaaacgta aaagaagtta tggatcaagc cttagaagaa tatatacgga aatatttacc
     1441 tgacaaactt taaaaggagt gaaaatgaaa tggcagttac gtatgaaaaa acatttgaaa
     1501 tagagatcat taacgaatta tcggcaagcg tttataatcg agtattaaac tatgttttga
     1561 accatgaatt aaataaaaat gactctcaat tattggaagt caatttatta aaccaattaa
     1621 agcttgcaaa acgtgtaaat ctttttgatt attctttaga agaattacaa gccgttcatg
     1681 agtattggcg gtcaatgaat cgttactcaa aacaagtttt gaataaagag aaagtggctt
     1741 aatatggcaa atatagtcaa ttttactgac aaacaatttg agaatcgctt aaatgataat
     1801 ttagaagaat tgattcaagg aaaaaaagcg gttgaatcgc caaccgcttt tttacttggt
     1861 gggcaaccag ggtcagggaa aaccagtttg cgatcagcaa tttttgaaga aacacaaggg
     1921 aatgttattg tcattgataa tgataccttt aaacaacagc accctaattt tgatgaacta
     1981 gtgaaacttt atgaaaaaga cgtagtaaaa cacgttaccc cttattctaa tcgcatgaca
     2041 gaagcgatca taagccgttt gagcgatcaa gggtataatt tggtgatcga aggtacagga
     2101 cgaacaacag acgttcctat tcaaaccgca acaatgcttc aagccaaagg ttatgaaaca
     2161 aaaatgtatg tcatggcagt acctaaaatc aactcatatt taggaacaat tgaacgatat
     2221 gaaaccatgt atgcagatga tccaatgaca gccagggcaa caccaaaaca agcgcatgat
     2281 attgttgtca aaaacttacc gaccaattta gaaacccttc ataaaacggg cttatttagc
     2341 gatataaggc tatataacag agaaggagta aaactctatt caagtttgga aacaccttct
     2401 attagtccaa aagaaacctt agaaaaagaa ttgaatcgta aagtatcagg gaaagaaatt
     2461 caaccgactt tagaacgaat agagcaaaaa atggttctaa ataaacacca agagacacct
     2521 gaatttaaag caattcaaca aaaattggaa agcttgcagc cacctacacc accaataccc
     2581 aaaacaccta aacttccagg tatttaaacc ttaaaaaagg aaaagagtag ttaccaaaaa
     2641 acggtaacta ctcttttttt ataaaaacat ttcctcatgt tggacctgca ggaattcgat
     2701 atcaagctta tcgataccgt cgacctcgag ggggggccca tcagtctgac gaccaagaga
     2761 gccataaaca ccaatagcct taacatcatc cccatattta tccaatattc gttccttaat
     2821 ttcatgaaca atcttcattc tttcttctct agtcattatt attggtccat tcactattct
     2881 cattcccttt tcagataatt ttagatttgc ttttctaaat aagaatattt ggagagcacc
     2941 gttcttattc agctattaat aactcgtctt cctaagcatc cttcaatcct tttaataaca
     3001 attatagcat ctaatcttca acaaactggc ccgtttgttg aactactctt taataaaata
     3061 atttttccgt tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc
     3121 gtcatcatct gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg
     3181 tatttctttt aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa
     3241 atcagacaaa cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac
     3301 aggatatttt gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg
     3361 tcgatttttt attaaaacgt ctcaaaatcg tttctgagac gttttagcgt ttatttcgtt
     3421 tagttatcgg cataatcgtt aaaacaggcg ttatcgtagc gtaaaagccc ttgagcgtag
     3481 cgtggctttg cagcgaagat gttgtctgtt agattatgaa agccgatgac tgaatgaaat
     3541 aataagcgca gcgtccttct atttcggttg gaggaggctc aagggagttt gagggaatga
     3601 aattccctca tgggtttgat tttaaaaatt gcttgcaatt ttgccgagcg gtagcgctgg
     3661 aaaatttttg aaaaaaattt ggaatttgga aaaaaatggg gggaaaggaa gcgaattttg
     3721 cttccgtact acgacccccc attaagtgcc gagtgccaat ttttgtgcca aaaacgctct
     3781 atcccaactg gctcaagggt ttgaggggtt tttcaatcgc caacgaatcg ccaacgtttt
     3841 cgccaacgtt ttttataaat ctatatttaa gtagctttat tgttgttttt atgattacaa
     3901 agtgatacac taattttata aaattatttg attggagttt tttaaatggt gatttcagaa
     3961 tcgaaaaaaa gagttatgat ttctctgaca aaagagcaag ataaaaaatt aacagatatg
     4021 gcgaaacaaa aaggtttttc aaaatctgcg gttgcggcgt tagctataga agaatatgca
     4081 agaaaggaat cagaacaaaa aaaataagcg aaagctcgcg tttttagaag gatacgagtt
     4141 ttcgctactt gtttttgata aggtaattat atcatggcta ttaaaaatac taaagctaga
     4201 aattttggat ttttattata tcctgactca attcctaatg attggaaaga aaaattagag
     4261 agtttgggcg tatctatggc tgtcagtcct ttacacgata tggacgaaaa aaaagataaa
     4321 gatacatgga atagtagtga tgttatacga aatggaaagc actataaaaa accacactat
     4381 cacgttatat atattgcacg aaatcctgta acaatagaaa gcgttaggaa caagattaag
     4441 cgaaaattgg ggaatagttc agttgctcat gttgagatac ttgattatat caaaggttca
     4501 tatgaatatt tgactcatga atcaaaggac gctattgcta agaataaaca tatatacgac
     4561 aaaaaagata ttttgaacat taatgatttt gatattgacc gctatataac acttgatgaa
     4621 agccaaaaaa gaaaattgaa gaatttactt ttagatatag tggatgacta taatttggta
     4681 aatacaaaag atttaatggc ttttattcgc cttaggggag cggagtttgg aattttaaat
     4741 acgaatgatg taaaagatat tgtttcaaca aactctagcg cctttagatt atggtttgag
     4801 ggcaattatc agtgtggata tagagcaagt tatgcaaagg ttcttgatgc tgaaacgggg
     4861 gaaataaaat gacaaacaaa gaaaaagagt tatttgctga aaatgaggaa ttaaaaaaag
     4921 aaattaagga cttaaaagag cgtattgaaa gatacagaga aatggaagtt gaattaagta
     4981 caacaataga tttattgaga ggagggatta ttgaataaat aaaagccccc ctgacgaaag
     5041 tcgacgatac aaattcctcg taggcgctcg ggacccctat ctagcgaact tttagaaaag
     5101 atataaaaca tcagagtatg gacagttgcg gatgtacttc agaaaagatt agatgtctaa
     5161 aaagcttgta gttaaagctt tttagacatc taaatctagg tactaaaaca attcatccag
     5221 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     5281 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     5341 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     5401 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     5461 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     5521 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     5581 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     5641 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     5701 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     5761 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     5821 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     5881 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     5941 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     6001 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
     6061 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
     6121 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
     6181 caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca
     6241 tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct
     6301 tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc
     6361 agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg
     6421 gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc
     6481 atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat cgtcgacaag
     6541 ttttgggatt gttaagggtt ccgaggctca acgtcaataa agcaattgga ataaagaagc
     6601 gaaaaaggag aagtcggttc agaaaaagaa ggatatggat ctggagctgt aatataaaaa
     6661 ccttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa aaagtacagt
     6721 cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt cctgaataga
     6781 gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct gtaaagatag
     6841 cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa tgggtagaag
     6901 gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc atgctggcga
     6961 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     7021 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc
     7081 tctcgaaaag ccctgacaac ccttgttcct aaaaaggaat aagcgttcgg tcagtaaata
     7141 atagaaataa aaaatcagac ctaagactga tgacaaaaag agaaaatttt gataaaatag
     7201 tcttagaatt aaattaaaaa gggaggccaa atataatgaa aaatatgaat gacaatgatg
     7261 ttccgcggtg gcggccgcaa ggaggaataa aaaatgaaat ttggaaactt tttgcttaca
     7321 taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc
     7381 atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt
     7441 ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg
     7501 aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat
     7561 gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt
     7621 tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa
     7681 tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa
     7741 catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg
     7801 gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg
     7861 atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat
     7921 gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata
     7981 acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat
     8041 tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt
     8101 tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat
     8161 cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac
     8221 ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct
     8281 aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca
     8341 tttcttaaag aaaaacaacg ttcgctatta tattaagtcg aggaggagag aaagaaatga
     8401 aatttggatt gttcttcctt aacttcatca attcaacaac tgttcaagaa caaagtatag
     8461 ttcgcatgca ggaaataacg gagtatgttg ataagttgaa ttttgaacag attttagtgt
     8521 atgaaaatca tttttcagat aatggtgttg tcggcgctcc tctgactgtt tctggttttc
     8581 tgctcggttt aacagagaaa attaaaattg gttcattaaa tcacatcatt acaactcatc
     8641 atcctgtccg catagcggag gaagcttgct tattggatca gttaagtgaa gggagattta
     8701 ttttagggtt tagtgattgc gaaaaaaaag atgaaatgca tttttttaat cgcccggttg
     8761 aatatcaaca gcaactattt gaagagtgtt atgaaatcat taacgatgct ttaacaacag
     8821 gctattgtaa tccagataac gatttttata gcttccctaa aatatctgta aatccccatg
     8881 cttatacgcc aggcggacct cggaaatatg taacagcaac cagtcatcat attgttgagt
     8941 gggcggccaa aaaaggtatt cctctcatct ttaagtggga tgattctaat gatgttagat
     9001 atgaatatgc tgaaagatat aaagccgttg cggataaata tgacgttgac ctatcagaga
     9061 tagaccatca gttaatgata ttagttaact ataacgaaga tagtaataaa gctaaacaag
     9121 agacgcgtgc atttattagt gattatgttc ttgaaatgca ccctaatgaa aatttcgaaa
     9181 ataaacttga agaaataatt gcagaaaacg ctgtcggaaa ttatacggag tgtataactg
     9241 cggctaagtt ggcaattgaa aagtgtggtg cgaaaagtgt attgctgtcc tttgaaccaa
     9301 tgaatgattt gatgagccaa aaaaatgtaa tcaatattgt tgatgataat attaagaagt
     9361 accacatgga atatacctaa gtcgaggagg atggcaaata tgactaaaaa aatttcattc
     9421 attattaacg gccaggttga aatctttccc gaaggtgatg atttagtgca atccattaat
     9481 tttggtgata atagtgttta cctgccaata ttgaatgact ctcatgtaaa aaacattatt
     9541 gattgtaatg gaaataacga attacggttg cataacattg tcaattttct ctatacggta
     9601 gggcaaagat ggaaaaatga agaatactca agacgcagga catacattcg tgacttaaaa
     9661 aaatatatgg gatattcaga agaaatggct aagctagagg ccaattggat atctatgatt
     9721 ttatgttcta aaggcggcct ttatgatgtt gtagaaaatg aacttggttc tcgccatatc
     9781 atggatgaat ggctacctca ggatgaaagt tatgttcggg cttttccgaa aggtaaatct
     9841 gtacatctgt tggcaggtaa tgttccacta tctgggatca tgtctatatt acgcgcaatt
     9901 ttaactagga atcagtgtat tataaaaaca tcgtcaaccg atccttttac cgctaatgca
     9961 ttagcgttaa gttttattga tgtagacccc aatcatccga taacgcgctc tttatctgtt
    10021 atatattggc cccaccaagg tgatacatca ctcgcaaaag aaattatgca acatgcggat
    10081 gttattgtcg cttggggagg gccagatgcg attaattggg cggtagagca tgcgccatct
    10141 tatgctgatg tgattaaatt tggttctaaa aagagtcttt gcattatcga taatcctgtt
    10201 gatttgacgt ccgcagcgac aggtgcggct catgatgttt gtttttacga tcagcgagct
    10261 tgtttttctg cccaaaacat atattacatg ggaaatcatt atgaggaatt taagttagcg
    10321 ttgatagaaa aacttaatct atatgcgcat atattaccga atgccaaaaa agattttgat
    10381 gaaaaggcgg cctattcttt agttcaaaaa gaaagcttgt ttgctggatt aaaagtagag
    10441 gtggatattc atcaacgttg gatgattatt gagtcaaatg caggtgtgga atttaatcaa
    10501 ccacttggca gatgtgtgta ccttcatcac gtcgataata ttgagcaaat attgccttat
    10561 gttcaaaaaa ataagacgca aaccatatct atttttcctt gggagtcatc atttaaatat
    10621 cgagatgcgt tagcattaaa aggtgcggaa aggattgtag aagcaggaat gaataacata
    10681 tttcgagttg gtggatctca tgacggaatg agaccgttgc aacgattagt gacatatatt
    10741 tctcatgaaa ggccatctaa ctatacggct aagaatgttg cggttgaaat agaacagact
    10801 cgattcctgg aagaagataa gttccttgta tttgtcccat aagtcgagga ggagtaaaag
    10861 tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa
    10921 aaaaattcat gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat
    10981 tattattgcg tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt
    11041 atcgcggaat ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc
    11101 agggacaatt gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga
    11161 ttggttaact acacgaaaaa taaataactt cggtatgttg gcttcaagct tatctgcgcg
    11221 gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt
    11281 tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat
    11341 taatgaattg ccgaataatc tagattttga aggccataaa ttgggtgctg aagtctttgc
    11401 gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta
    11461 tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga
    11521 agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg
    11581 aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac
    11641 gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc
    11701 gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga
    11761 aaatcaagca atttctctgt cttaagtcga ggaggaaaac aggtatgact tcatatgttg
    11821 ataaacaaga aattacagca agctcagaaa ttgatgattt gattttttcg agcgatccat
    11881 tagtgtggtc ttacgacgag caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc
    11941 gtaatcatta taaacattgt cgagaatatc gtcactactg tcaggcacac aaagtagatg
    12001 acaatattac ggaaattgat gacatacctg tattcccaac atcggttttt aagtttactc
    12061 gcttattaac ttctcaggaa aacgagattg aaagttggtt taccagtagc ggcacgaatg
    12121 gtttaaaaag tcaggtggcg cgtgacagat taagtattga gagactctta ggctctgtga
    12181 gttatggcat gaaatatgtt ggtagttggt ttgatcatca aatagaatta gtcaatttgg
    12241 gaccagatag atttaatgct cataatattt ggtttaaata tgttatgagt ttggtggaat
    12301 tgttatatcc tacgacattt accgtaacag aagaacgaat agattttgtt aaaacattga
    12361 atagtcttga acgaataaaa aatcaaggga aagatctttg tcttattggt tcgccatact
    12421 ttatttattt actctgccat tatatgaaag ataaaaaaat ctcattttct ggagataaaa
    12481 gcctttatat cataaccgga ggcggctgga aaagttacga aaaagaatct ctgaaacgtg
    12541 atgatttcaa tcatctttta tttgatactt tcaatctcag tgatattagt cagatccgag
    12601 atatatttaa tcaagttgaa ctcaacactt gtttctttga ggatgaaatg cagcgtaaac
    12661 atgttccgcc gtgggtatat gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg
    12721 atggaacgcc ggggttgatg agttatatgg atgcgtcagc aaccagttat ccagcattta
    12781 ttgttaccga tgatgtcggg ataattagca gagaatatgg taagtatccc ggcgtgctcg
    12841 ttgaaatttt acgtcgcgtc aatacgagga cgcagaaagg gtgtgcttta agcttaaccg
    12901 aagcgtttga tagttgaggc ggcccaaaac aatatgatga ctggttgctg ttacatattt
    12961 ccgaggtccg cctggcgtat aagcatgggg atttacagat attttaggga agctataaaa
    13021 atcgttatct ggattacaat agcctgttgt taaagcatcg ttaatgattt cataacactc
    13081 ttcaaatagt tgctgttgat attcaaccgg gcgattaaaa aaatgcattt catctttttt
    13141 ttcgcaatca ctaaacccta aaataaatct cccttcactt aactgatcca ataagcaagc
    13201 ttcctccgct atgcggacag gatgatgagt tgtaatgatg tgatttaatg aaccaatttt
    13261 aattttctct gttaaaccga gcagaaaacc agaaacagtc agaggagcgc cgacaacacc
    13321 attatctgaa aaatgatttt catacactaa aatctgttca aaattcaact tatcaacata
    13381 ctccgttatt tcctgcatgc gaactatac
//
