LOCUS       sequence-174996-        7516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For  expression of PLEKHM1 tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
  TITLE     Atg8 family LC3/GABARAP proteins are crucial for 
            autophagosome-lysosome fusion but not autophagosome formation during
            PINK1/Parkin mitophagy and starvation.
  JOURNAL   J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.
  PUBMED    27864321
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-12-19"; volume: "215(6)"; pages: "857-874. Epub 
            2016 Nov 18"
COMMENT     Sequence Label: pBMN PLEKHM1-HA
FEATURES             Location/Qualifiers
     source          1..7516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             4934..4960
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             5134..5603
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5733..5750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5904..6492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5984..6003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6656..7447,7448..7516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7279..7298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatccatgct ttcagtggtg gagaatggac tggaccccca
     1801 ggctgccatc ccggtcatca agaagaagct ggtgggatcc gtgaaggcct tgcagaagca
     1861 gtacgtgtcc ctggacacgg tggtcactag tgaagacgga gatgccaaca ccatgtgcag
     1921 cgccctggag gccgtattta tccatggcct gcacgccaag cacatccgag ctgaggccgg
     1981 aggaaaaagg aagaaaagtg cccaccagaa gcctctgccc cagcctgtct tctggcccct
     2041 cctgaaagct gtcacccaca aacacatcat ctcagagttg gagcacctga cgtttgtcaa
     2101 cacggatgtg ggccgctgcc gggcatggct gcggctggcc ctgaacgatg gcctgatgga
     2161 gtgctacctg aagctgctgc tgcaggagca ggcccgcttg catgagtact accagcccac
     2221 cgccctgctc cgggatgctg aggagggcga gttcctcctt agcttcctgc agggcctcac
     2281 gtccttgtcc ttcgaactct cctacaagtc tgccatctta aatgagtgga cgctcacccc
     2341 attggccctg tctgggcttt gcccgctttc tgagctggac cctctctcta cctctggtgc
     2401 agaactacag cggaaggaat ctctggattc catttcccat tcttcaggct ctgaagacat
     2461 cgaagtccat cactcgggcc ataagatacg gaggaaccag aagctgactg cctcctccct
     2521 cagcctggac acggccagtt catcccagct gtcctgcagc ctaaactctg atagctgctt
     2581 actccaagag aatggctcca agagtccaga ccattgcgag gagcccatgt cctgtgactc
     2641 agacctgggc acagcaaatg ctgaggactc agaccggtct ctgcaagagg tattgttgga
     2701 attcagcaaa gcccaggtaa actctgtgcc aaccaacgga ctgagccaag aaacagagat
     2761 ccccacacca caggcctcgc tctccctcca tggcctcaac accagcacat acctgcactg
     2821 tgaggcacct gcagagcccc ttcctgccca ggcagcctct ggaactcaag atggtgtcca
     2881 cgtgcaggag ccgcgtcccc aggcgcccag ccccctggac ttacagcagc ctgtagagag
     2941 cacctcaggc cagcagcctt ctagtactgt cagcgagaca gccagagaag tgggccaagg
     3001 gaatggcctg cagaaggccc aggctcatga cggagctggt ctgaagctgg tagtttcctc
     3061 acccaccagt ccgaaaaaca agagctggat ctcagaggat gacttctacc ggccttcccg
     3121 ggagcaaccc ctggagagtg cttcagacca cccaatagct tcttacaggg ggactccagg
     3181 gtcaaggcct ggtctccaca ggcatttttc tcaagaacca agaaaaaact gctccctggg
     3241 ggcgttagac caagcgtgtg taccttcccc aggaagaagg caagcccagg cagccccatc
     3301 ccaggggcat aagagcttcc gggtggtaca ccggagacag atgggactgt ccaacccatt
     3361 ccggggtctc atgaagctgg gcaccgtgga gcggcggggg gcaatgggca tctggaagga
     3421 gctcttctgc gagctctccc cgctggagtt ccgcctctac ctgagcaacg aggagcacac
     3481 ctgtgtggag aactgctcgc tgcttcgctg tgagtctgtg gggccagccc atagtgatgg
     3541 gcgctttgag ctggtcttct ctggcaagaa gctggccctg cgcgcctcct cccaggacga
     3601 agctgaggac tggctggacc gggtgcggga ggccctgcag aaggtccggc ctcagcagga
     3661 ggatgagtgg gtgaacgtgc agtacccaga ccagcctgag gaaccccccg aggcgcccca
     3721 gggctgcctc tctccctcag acctgctctc ggagcccgcg gccctccagg gcacacagtt
     3781 tgactggtcg tccgcccagg ttccagagcc agatgccatc aaggagtccc tgctgtactt
     3841 gtacatggac aggacctgga tgccctatat attttctctg tccttggagg ctctgaaatg
     3901 tttccgcatc aggaacaatg agaagatgct gagtgacagc cacggcgtgg agaccatccg
     3961 ggacatcctg ccagacacca gccttggggg cccatccttc ttcaaaatca tcacggccaa
     4021 ggctgtcctg aagctgcagg ccggaaacgc cgaggaagcc gccctgtgga gggatctggt
     4081 ccgcaaagtc ctggcatcct acttggagac agccgaggag gcggtgaccc tgggcgggag
     4141 cctggatgaa aactgtcagg aggtgctgaa atttgccacc cgggagaatg gcttcctgct
     4201 gcagtacctg gtggctatcc ccatggagaa aggccttgac tcccaaggct gcttctgcgc
     4261 aggctgctcc cggcagatcg gcttctcctt tgtacgaccc aagctctgtg ccttctctgg
     4321 cctctattac tgtgacatct gccaccaaga cgatgcctca gtgattccgg ccaggatcat
     4381 ccacaactgg gacctcacca agcgcccgat ctgcaggcag gccctgaagt ttctgacaca
     4441 gatccgggcc cagcccctca tcaacctgca gatggtgaac gcgtctctgt acgagcatgt
     4501 ggagcggatg cacctcattg ggaggagacg ggagcagctg aagctcctgg gggattacct
     4561 gggcctgtgc cggagtggcg ccctgaagga gctcagcaag aggctcaacc acaggaatta
     4621 tctcttggaa tctccgcata ggttcagtgt tgctgacctc caacagatcg cagacggggt
     4681 gtatgaagga ttcctcaagg ccctgattga atttgcctcc cagcatgtct accactgcga
     4741 cctgtgcacc cagcgcggct tcatctgcca gatctgccag caccacgaca tcatcttccc
     4801 ctttgagttt gacaccacag tcaggtgtgc cgagtgcaag accgtcttcc accagagctg
     4861 ccaggctgtg gtgaagaagg gctgcccccg ctgtgcccgc cggcgcaagt accaggaaca
     4921 gaacattttc gcctacccat acgatgttcc agattacgct taagtcgacg ataaaataaa
     4981 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5041 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     5101 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     5161 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     5221 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     5281 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     5341 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5401 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     5461 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     5521 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     5581 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     5641 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     5701 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5761 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5821 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6541 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6601 ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7501 aatgttgaat actcat
//
