LOCUS       sequence-174998-        3360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of the mammalian Atg8 protein GABARAPL2 without a 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
  TITLE     Atg8 family LC3/GABARAP proteins are crucial for 
            autophagosome-lysosome fusion but not autophagosome formation during
            PINK1/Parkin mitophagy and starvation.
  JOURNAL   J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.
  PUBMED    27864321
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-12-19"; volume: "215(6)"; pages: "857-874. Epub 
            2016 Nov 18"
COMMENT     Sequence Label: pMX-IY GBRPL2
FEATURES             Location/Qualifiers
     source          1..3360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(44..835,836..904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     667..686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(905..1009)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1039..1632
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1695..2052
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2111..2527
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2457..2479
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2495..2511
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2537..2911
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2849..2865
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
       61 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
      541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
      601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
      661 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
      721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1021 gaaaagtgcc acctgcataa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     1081 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag
     1141 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     1201 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     1261 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     1321 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     1381 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     1441 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     1501 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     1561 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     1621 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     1681 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     1741 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     1801 acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg
     1861 gacttcgggg gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact
     1921 ctttggtgca cccccctaat aggagggata tgtggttctg gtaggagacg agaacctaaa
     1981 acagttcccg cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg
     2041 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     2101 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     2161 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     2221 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     2281 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     2341 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     2401 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     2461 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     2521 aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt
     2581 ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc
     2641 tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact
     2701 ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca
     2761 ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc
     2821 caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc
     2881 cgaccccggg ggtggaccat cctctagact gccggatcta gctagttaat taaggatccc
     2941 agtgtggtgg tacggaattc caccatgaag tggatgttca aggaggacca ctcgctggaa
     3001 cacagatgcg tggagtccgc gaagattcga gcgaaatatc ccgacagggt tccggtgatt
     3061 gtggaaaagg tctcaggctc tcagattgtt gacattgaca aacggaagta cttggttcca
     3121 tctgatatca ctgtggctca gttcatgtgg atcatcagga aaaggatcca gcttccttct
     3181 gaaaaggcga tcttcctgtt tgtggataag acagtcccac agtccagcct aactatggga
     3241 cagctttacg agaaggaaaa agatgaagat ggattcttat atgtggccta cagcggagag
     3301 aacacttttg gcttctgact cgagggccgg cgcgccgcgg ccgctacgta aattccgccc
//
