LOCUS       sequence-17500-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J
  TITLE     Molecular interpretation of ERK signal duration by immediate early 
            gene products.
  JOURNAL   Nat Cell Biol. 2002 Aug . 4(8):556-64.
  PUBMED    12134156
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Aug . 4(8):556-64."
COMMENT     Sequence Label: pcDNA3-FLAG-Fos DD F343A
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             137..160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc accatggact acaaagacga tgacgacaag ttctcgggtt tcaacgcgga
      181 ctacgaggcg tcatcctccc gctgcagtag cgcctccccg gccggggaca gcctttccta
      241 ctaccattcc ccagccgact ccttctccag catgggctcc cctgtcaaca cacaggactt
      301 ttgcgcagat ctgtccgtct ctagtgccaa ctttatcccc acggtgacag ccatctccac
      361 cagcccagac ctgcagtggc tggtgcagcc cactctggtc tcctccgtgg ccccatcgca
      421 gaccagagcg ccccatcctt acggactccc caccccgtcg accggggctt acgccagagc
      481 gggagtggtg aagaccatgt caggcggcag agcgcagagc atcggcagaa ggggcaaagt
      541 agagcagcta tctcctgaag aggaagagaa acggagaatc cgaagggaaa ggaataagat
      601 ggctgcagcc aagtgccgga atcggaggag ggagctgaca gatacgctcc aagcggagac
      661 agatcaactt gaagacgaga agtctgcgtt gcagaccgag attgccaatc tactgaaaga
      721 gaaggaaaaa ctggagttta ttttggcagc ccaccgacct gcctgcaaga tccccaatga
      781 cctgggcttc ccagaggaga tgtctgtgac ctccctggac ttgactgggg gtctgcctga
      841 ggctaccacc ccagagtctg ag
//
