LOCUS       sequence-175011-        1935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of the mammalian Atg8 protein GABARAPL2 tagged with 
            HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
  TITLE     Atg8 family LC3/GABARAP proteins are crucial for 
            autophagosome-lysosome fusion but not autophagosome formation during
            PINK1/Parkin mitophagy and starvation.
  JOURNAL   J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.
  PUBMED    27864321
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-12-19"; volume: "215(6)"; pages: "857-874. Epub 
            2016 Nov 18"
COMMENT     Sequence Label: pMX-IY HA-GBRPL2
FEATURES             Location/Qualifiers
     source          1..1935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    12..564
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(156..173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     383..402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(576..578,579..581,582..1295)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(621..642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(882..901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1229..1250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1466..1935
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
       61 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      121 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      181 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      241 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      301 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      361 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      421 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
      481 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
      601 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      661 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      721 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
      781 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      841 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      901 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      961 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1021 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1081 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga
     1201 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1261 ggatcactct cggcatggac gagctgtaca agtaagtcga cgataaaata aaagatttta
     1321 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     1381 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     1441 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     1501 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     1561 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     1621 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     1681 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     1741 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     1801 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     1861 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     1921 agcgggggtc tttca
//
